DyLight® 488荧光Anti-6X His tag®抗体[AD1.1.10] (ab117512)
Key features and details
- DyLight® 488 Mouse monoclonal [AD1.1.10] to 6X His tag®
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Species independent
- Conjugation: DyLight® 488. Ex: 493nm, Em: 518nm
- Isotype: IgG1
Related conjugates and formulations
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
DyLight® 488荧光Anti-6X His tag®抗体[AD1.1.10]
参阅全部 6X His tag® 一抗 -
描述
DyLight® 488荧光小鼠单克隆抗体[AD1.1.10] to 6X His tag® -
宿主
Mouse -
偶联物
DyLight® 488. Ex: 493nm, Em: 518nm -
经测试应用
适用于: ICC/IF, Flow Cytmore details -
种属反应性
与反应: Species independent -
免疫原
Synthetic peptide of 6X His tag®.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.1% BSA, 0.88% Sodium chloride, 80.5% Dibasic monohydrogen sodium phosphate, 18.5% Monobasic dihydrogen sodium phosphate -
Concentration information loading... -
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
AD1.1.10 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
- PerCP Anti-6X His tag® antibody [AD1.1.10] (ab117496)
- DyLight® 650 Anti-6X His tag® antibody [AD1.1.10] (ab117504)
- DyLight® 550 Anti-6X His tag® antibody [AD1.1.10] (ab117507)
- Biotin Anti-6X His tag® antibody [AD1.1.10] (ab15145)
- Anti-6X His tag® antibody [AD1.1.10] (ab15149)
- HRP Anti-6X His tag® antibody [AD1.1.10] (ab178563)
- PE Anti-6X His tag® antibody [AD1.1.10] (ab72467)
- SureLight® APC Anti-6X His tag® antibody [AD1.1.10] (ab72579)
- Alkaline Phosphatase Anti-6X His tag® antibody [AD1.1.10] (ab81652)
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab117512于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/500 - 1/5000.
|
|
| Flow Cyt |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
ICC/IF
1/500 - 1/5000. |
|
Flow Cyt
Use at an assay dependent concentration. |
靶标
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相关性
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. His-tags can be fused to the amino- or carboxy- termini of proteins in transfected or transformed cells. -
细胞定位
Depends upon the localization of the parent protein tagged with hexahistidine. -
别名
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
see all
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab117512 被引用在 6 文献中.
- Srila W et al. Production and applications of fluorobody from redox-engineered Escherichia coli. Appl Microbiol Biotechnol 107:1959-1970 (2023). PubMed: 36729226
- Sumphanapai T et al. Targeting acute myeloid cell surface using a recombinant antibody isolated from whole-cell biopanning of a phage display human scFv antibody library. Med Oncol 39:205 (2022). PubMed: 36175701
- Min TT & Yamabhai M Human Hexa-Histidine-Tagged Single-Chain Variable Fragments for Bioimaging of Bacterial Infections. ACS Omega 6:762-774 (2021). PubMed: 33458528
- Ding Y et al. Optimized Anti-Prostate-Specific Membrane Antigen Single-Chain Variable Fragment-Loaded Nanobubbles as a Novel Targeted Ultrasound Contrast Agent for the Diagnosis of Prostate Cancer. J Ultrasound Med 39:761-773 (2020). PubMed: 31702068
- Sirois AR et al. Engineered Fn3 protein has targeted therapeutic effect on mesothelin-expressing cancer cells and increases tumor cell sensitivity to chemotherapy. Biotechnol Bioeng 117:330-341 (2020). PubMed: 31631324
- Imhof BA et al. CCN1/CYR61-mediated meticulous patrolling by Ly6Clow monocytes fuels vascular inflammation. Proc Natl Acad Sci U S A 113:E4847-56 (2016). PubMed: 27482114