AB229122

Anti-dUTPase抗体

Name: dUTPase抗体 (ab229122)| Abcam中文官网
Brand: Abcam Limited
SKU: AB229122
Price: 4748 CNY
Availability: InStock

Anti-dUTPase antibody

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(3 Publications)

Rabbit Polyclonal dUTPase antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DUT.

查看别名

dUTPase, dUTP pyrophosphatase, DUT

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-dUTPase antibody (AB229122)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-dUTPase antibody (AB229122)

Paraffin-embedded A549 xenograft tissue stained for dUTPase using ab229122 at 1/500 dilution in immunohistochemical analysis.

Supplier Data

Immunoprecipitation - Anti-dUTPase antibody (AB229122)

dUTPase was immunoprecipitated from A431 (human epidermoid carcinoma cell line) whole cell extract with 5 μg ab229122. Western blot was performed from the immunoprecipitate using ab229122. Anti-Rabbit IgG was used as a secondary reagent.

Lane 1 : Control IgG IP in A431 whole cell extract.
Lane 2 : ab229122 IP in A431 whole cell extract.

All lanes:

Immunoprecipitation - Anti-dUTPase antibody (ab229122)

Predicted band size: 26 kDa

false

Supplier Data

Western blot - Anti-dUTPase antibody (AB229122)

15% SDS-PAGE gel.

All lanes:

Western blot - Anti-dUTPase antibody (ab229122) at 1/1000 dilution

All lanes:

A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg

Predicted band size: 26 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

IP, IHC-P, WB

applications

免疫原

Recombinant Fragment Protein within Human DUT. The exact immunogen used to generate this antibody is proprietary information.

P33316

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The enzyme deoxyuridine triphosphatese also known as dUTPase hydrolyzes dUTP to dUMP and inorganic pyrophosphate. It prevents the incorporation of uracil into DNA which can lead to mutations. The molecular mass of human dUTPase is about 18-20 kDa. It is widely expressed in both prokaryotic and eukaryotic cells including places like the thymus and spleen where DNA synthesis is very active. Alternative names such as DUT or dUTP pyrophosphatase are often used to refer to this enzyme.

Biological function summary

DUTPase maintains nucleotide pool balance and genome integrity. By converting dUTP to dUMP it provides substrates for thymidylate synthase to produce thymidine triphosphate (dTTP) which is essential for DNA replication and repair. dUTPase is not typically part of a larger protein complex but cooperates with other enzymes in nucleotide metabolism. Its activity protects cells from apoptosis that might occur due to extensive uracil misincorporation.

Pathways

DUTPase plays a role in nucleotide metabolism and one-carbon metabolism pathways. It regulates the pyrimidine metabolism pathway ensuring sufficient dTTP levels for DNA synthesis. dUTPase interacts indirectly with proteins like thymidylate synthase and dihydrofolate reductase through these pathways. These interactions aid in maintaining a steady supply of nucleotides important for DNA replication and repair.

Impairments in dUTPase function can relate to cancer and immunodeficiency disorders. In some cancers altered dUTPase expression affects the sensitivity to chemotherapeutic agents particularly those targeting thymidylate synthesis. In certain immunodeficiencies dUTPase malfunction leads to increased uracil incorporation compromising DNA integrity. The enzyme connects with proteins such as thymidylate synthase in these diseases due to its role in managing nucleotide pools critical for cell proliferation.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Catalyzes the cleavage of 2'-deoxyuridine 5'-triphosphate (dUTP) into 2'-deoxyuridine 5'-monophosphate (dUMP) and inorganic pyrophosphate and through its action efficiently prevents uracil misincorporation into DNA and at the same time provides dUMP, the substrate for de novo thymidylate biosynthesis (PubMed : 17880943, PubMed : 8631816, PubMed : 8805593). Inhibits peroxisome proliferator-activated receptor (PPAR) activity by binding of its N-terminal to PPAR, preventing the latter's dimerization with retinoid X receptor (By similarity). Essential for embryonic development (By similarity).

See full target information DUT

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 131: PubMed33630758

2021

Deficiency of macrophage PHACTR1 impairs efferocytosis and promotes atherosclerotic plaque necrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Canan Kasikara,Maaike Schilperoort,Brennan Gerlach,Chenyi Xue,Xiaobo Wang,Ze Zheng,George Kuriakose,Bernhard Dorweiler,Hanrui Zhang,Gabrielle Fredman,Danish Saleheen,Muredach P Reilly,Ira Tabas

Molecular cancer therapeutics 20:64-75 PubMed33087508

2020

Reprogramming of Nucleotide Metabolism Mediates Synergy between Epigenetic Therapy and MAP Kinase Inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Tatiana Shorstova,Jie Su,Tiejun Zhao,Michael Dahabieh,Matthew Leibovitch,Mariana De Sa Tavares Russo,Daina Avizonis,Shivshankari Rajkumar,Ian R Watson,Sonia V Del Rincón,Wilson H Miller,William D Foulkes,Michael Witcher

Cancer cell 37:340-353.e6 PubMed32109375

2020

CDK4/6 Inhibitors Impair Recovery from Cytotoxic Chemotherapy in Pancreatic Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Beatriz Salvador-Barbero,Mónica Álvarez-Fernández,Elisabet Zapatero-Solana,Aicha El Bakkali,María Del Camino Menéndez,Pedro P López-Casas,Tomas Di Domenico,Tao Xie,Todd VanArsdale,David J Shields,Manuel Hidalgo,Marcos Malumbres

View all publications

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Anti-dUTPase抗体

Anti-dUTPase antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-dUTPase antibody (AB229122)

Immunoprecipitation - Anti-dUTPase antibody (AB229122)

Western blot - Anti-dUTPase antibody (AB229122)