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AB326708

Anti-DUSP6 antibody [9C3]

Anti-DUSP6 antibody [9C3]

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Rabbit Recombinant Monoclonal DUSP6 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples.

查看别名

MKP3, PYST1, DUSP6, Dual specificity protein phosphatase 6, Dual specificity protein phosphatase PYST1, Mitogen-activated protein kinase phosphatase 3, MAP kinase phosphatase 3, MKP-3

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DUSP6 antibody [9C3] (AB326708)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DUSP6 antibody [9C3] (AB326708)

Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DUSP6 Monoclonal Antibody, Unconjugated (ab326708) at 1 : 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated and DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DUSP6 antibody [9C3] (AB326708)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DUSP6 antibody [9C3] (AB326708)

Paraformaldehyde-fixed, paraffin embedded Human Thyroid Gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DUSP6 Monoclonal Antibody, Unconjugated (ab326708) at 1 : 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated and DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DUSP6 antibody [9C3] (AB326708)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DUSP6 antibody [9C3] (AB326708)

Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DUSP6 Monoclonal Antibody, Unconjugated (ab326708) at 1 : 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated and DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DUSP6 antibody [9C3] (AB326708)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DUSP6 antibody [9C3] (AB326708)

Paraformaldehyde-fixed, paraffin embedded Rat Thyroid Gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DUSP6 Monoclonal Antibody, Unconjugated (ab326708) at 1 : 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated and DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DUSP6 antibody [9C3] (AB326708)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DUSP6 antibody [9C3] (AB326708)

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DUSP6 Monoclonal Antibody, Unconjugated (ab326708) at 1 : 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated and DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DUSP6 antibody [9C3] (AB326708)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DUSP6 antibody [9C3] (AB326708)

Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DUSP6 Monoclonal Antibody, Unconjugated (ab326708) at 1 : 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated and DAB staining.

Western blot - Anti-DUSP6 antibody [9C3] (AB326708)
  • WB

Supplier Data

Western blot - Anti-DUSP6 antibody [9C3] (AB326708)

25 ug total protein per lane of various lysates (see on figure) probed with DUSP6 monoclonal antibody, unconjugated (ab326708) at 1 : 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

All lanes:

Western blot - Anti-DUSP6 antibody [9C3] (ab326708) at 1/1000 dilution

Lane 1:

NIH/3T3 at 25 µg

Lane 2:

Rat Thyroid gland at 25 µg

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关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

9C3

亚型

IgG

不含载体蛋白

No

反应种属

Human, Mouse, Rat

应用

IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
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产品详情

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 1% TBS, 1X
运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
储存信息
Avoid freeze / thaw cycle|The product can be stored for up to 12 months
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Dual specificity protein phosphatase, which mediates dephosphorylation and inactivation of MAP kinases (PubMed : 8670865). Has a specificity for the ERK family (PubMed : 8670865). Plays an important role in alleviating chronic postoperative pain (By similarity). Necessary for the normal dephosphorylation of the long-lasting phosphorylated forms of spinal MAPK1/3 and MAP kinase p38 induced by peripheral surgery, which drives the resolution of acute postoperative allodynia (By similarity). Also important for dephosphorylation of MAPK1/3 in local wound tissue, which further contributes to resolution of acute pain (By similarity). Promotes cell differentiation by regulating MAPK1/MAPK3 activity and regulating the expression of AP1 transcription factors (PubMed : 29043977).
See full target information DUSP6
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Abcam Product Promise

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