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重组RabMAb

重组Anti-DUSP3抗体[EPR5492] (ab125077)

Submit a review Q&A (1)References (1)
Western blot - Anti-DUSP3 antibody [EPR5492] (ab125077)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DUSP3 antibody [EPR5492] (ab125077)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DUSP3 antibody [EPR5492] (ab125077)
  • Anti-DUSP3 antibody [EPR5492] (ab125077)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5492] to DUSP3
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-DUSP3抗体[EPR5492]
    参阅全部 DUSP3 一抗

  • 描述

    兔单克隆抗体[EPR5492] to DUSP3

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IHC-Pmore details

  • 种属反应性

    与反应: Mouse, Human
    预测可用于: Rat

  • 免疫原

    Synthetic peptide within Human DUSP3 aa 1-100. The exact sequence is proprietary.

  • 阳性对照

    • HeLa, Jurkat, MCF7 and K562 cell lysates; Human ovarian cancer tissue and Mouse kidney tissue.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab125077于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/50000 - 1/200000. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
说明
WB
1/50000 - 1/200000. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能

    Shows activity both for tyrosine-protein phosphate and serine-protein phosphate, but displays a strong preference toward phosphotyrosines. Specifically dephosphorylates and inactivates ERK1 and ERK2.

  • 序列相似性

    Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
    Contains 1 tyrosine-protein phosphatase domain.

  • 细胞定位

    Nucleus.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • Dual specificity phosphatase 3 antibody
    • Dual specificity protein phosphatase 3 antibody
    • Dual specificity protein phosphatase VHR antibody
    • DUS3_HUMAN antibody
    • DUSP3 antibody
    • Serine/threonine specific protein phosphatase antibody
    • Vaccinia H1-related phosphatase antibody
    • Vaccinia virus phosphatase VH1 related antibody
    • VHR antibody
    see all

图片

  • Western blot - Anti-DUSP3 antibody [EPR5492] (ab125077)
    Western blot - Anti-DUSP3 antibody [EPR5492] (ab125077)
    All lanes : Anti-DUSP3 antibody [EPR5492] (ab125077) at 1/50000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : MCF-7 cell lysate
    Lane 4 : K562 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 20 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DUSP3 antibody [EPR5492] (ab125077)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DUSP3 antibody [EPR5492] (ab125077)

    ab125077, at 1/100, staining DUSP3 in paraffin-embedded Human ovarian cancer tissue by immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DUSP3 antibody [EPR5492] (ab125077)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DUSP3 antibody [EPR5492] (ab125077)

    ab125077, at 1/100, staining DUSP3 in paraffin-embedded Mouse kidney tissue by immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-DUSP3 antibody [EPR5492] (ab125077)
    Anti-DUSP3 antibody [EPR5492] (ab125077)

文献 (1)

发表研究结果有使用 ab125077？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab125077 被引用在 1 文献中.

  • Ye T  et al. Large extracellular vesicles secreted by human iPSC-derived MSCs ameliorate tendinopathy via regulating macrophage heterogeneity. Bioact Mater 21:194-208 (2023). PubMed: 36101856

客户评价及客户问答

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Question

Product code: 125077
Inquiry: Hi, Could you let me know where in the Dusp3 protein the Anti-DUSP3 antibody [EPR5492] binds to please? I don't need to know the exact sequence but I want to be sure it is downstream of my predicted knockout target exon Many thanks,

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Answer

Thank you for contacting us and your interest in our products.

The peptide used to raise the anti-DUSP3 antibody [EPR5492] (ab125077) has been taken from the region ofamino acids 1 - 30 of theN-terminus of human DUSP3/VHR (UniProt: P51452).


I hope this information has been sufficient to answer your question. If you have any further questions please do not hesitate to contact us again.

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