Anti-DUSP26抗体(ab224407)
Key features and details
- Rabbit polyclonal to DUSP26
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-DUSP26抗体 -
描述
兔多克隆抗体to DUSP26 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow, Orangutan
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免疫原
Recombinant fragment corresponding to Human DUSP26 aa 1-100.
Database link: Q9BV47 -
阳性对照
- IHC-P: Human prostate and cerebellum tissue. WB: DUSP26 overexpression HEK-293T lysate. ICC/IF: SH-SY5Y cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab224407于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 24 kDa.
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| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
| 说明 |
|---|
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 24 kDa. |
|
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
靶标
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功能
Inactivates MAPK1 and MAPK3 which leads to dephosphorylation of heat shock factor protein 4 and a reduction in its DNA-binding activity. Inhibits MAP kinase p38 by dephosphorylating it and inhibits p38-mediated apoptosis in anaplastic thyroid cancer cells. Can also induce activation of MAP kinase p38 and c-Jun N-terminal kinase (JNK). -
组织特异性
Brain. In the brain it is expressed ubiquitously except in the hippocampus. Expressed in embryonal cancers (retinoblastoma, neuroepithilioma and neuroblastoma) and in anaplatic thyroid cancer. -
序列相似性
Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
Contains 1 tyrosine-protein phosphatase domain. -
细胞定位
Cytoplasm. Nucleus. Golgi apparatus. - Information by UniProt
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数据库链接
- Entrez Gene: 533896 Cow
- Entrez Gene: 78986 Human
- Entrez Gene: 66959 Mouse
- Entrez Gene: 100173535 Orangutan
- Entrez Gene: 306527 Rat
- SwissProt: Q17QJ3 Cow
- SwissProt: Q9BV47 Human
- SwissProt: Q9D700 Mouse
see all -
别名
- DSP-4 antibody
- Dual specificity phosphatase 26 (putative) antibody
- Dual specificity phosphatase 26 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DUSP26 antibody (ab224407)
Paraffin-embedded human cerebellum tissue stained for DUSP26 using ab224407 at 1/50 dilution in immunohistochemical analysis.
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Western blot - Anti-DUSP26 antibody (ab224407)All lanes : Anti-DUSP26 antibody (ab224407) at 1/100 dilution
Lane 1 : Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) lysate
Lane 2 : DUSP26 overexpression HEK-293T (co-expressed with a C terminal myc-DDK tag, ~3.1 kDa) lysate
Predicted band size: 24 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DUSP26 antibody (ab224407)Paraffin-embedded human prostate tissue stained for DUSP26 using ab224407 at 1/50 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-DUSP26 antibody (ab224407)PFA-fixed, Triton X-100 permeabilized SH-SY5Y (human neuroblastoma cell line from bone marrow) cells stained for DUSP26 (green) using ab224407 at 4 µg/ml in ICC/IF.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab224407 被引用在 1 文献中.
- Chen J et al. Decreased DUSP26 Expression Promotes Malignant Behavior in Glioblastoma Cells via Deregulation of MAPK and Akt Signaling Pathway. Front Oncol 11:622826 (2021). PubMed: 33718185