Anti-DUSP14抗体(ab272587)
Key features and details
- Rabbit polyclonal to DUSP14
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-DUSP14抗体 -
描述
兔多克隆抗体to DUSP14 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Cow
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免疫原
Recombinant fragment corresponding to Human DUSP14 aa 1-150.
Database link: O95147 -
阳性对照
- WB: HEK-293T cells. IHC-P: Human small intestine tissue. ICC/IF: U-2 OS cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272587于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
|
|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
|
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Involved in the inactivation of MAP kinases. Dephosphorylates ERK, JNK and p38 MAP-kinases. -
序列相似性
Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
Contains 1 tyrosine-protein phosphatase domain. - Information by UniProt
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数据库链接
- Entrez Gene: 11072 Human
- Entrez Gene: 56405 Mouse
- Omim: 606618 Human
- SwissProt: O95147 Human
- SwissProt: Q9JLY7 Mouse
- Unigene: 91448 Human
- Unigene: 240885 Mouse
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别名
- Dual specificity phosphatase 14 antibody
- Dual specificity protein phosphatase 14 antibody
- DUS14_HUMAN antibody
see all
图片
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Western blot - Anti-DUSP14 antibody (ab272587)Lanes 2-3 : Anti-DUSP14 antibody (ab272587) at 0.4 µg/ml
Lane 1 : Molecular weight ladder
Lane 2 : Control (vector only transfected HEK-293T lysate)
Lane 3 : DUSP14 over-expression lysate (mammalian HEK-293T cells) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DUSP14 antibody (ab272587)Paraffin-embedded formalin-fixed Human small intestine tissue stained for DUSP14 using ab272587 at 1/200 in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-DUSP14 antibody (ab272587)PFA fixed, Triton X-100 permeablized human cell line U-2 OS labeling DUSP14 (green) using ab272587 at 2 ug/ml in ICC/IF analysis.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab272587 被引用在 1 文献中.
- Wu S et al. Osteoprotegerin deficiency aggravates methionine-choline-deficient diet-induced nonalcoholic steatohepatitis in mice. Sci Rep 13:3194 (2023). PubMed: 36823220