AB176727

Anti-DUBA抗体

Name: DUBA抗体 (ab176727)| Abcam中文官网
Brand: Abcam Limited
SKU: AB176727
Price: 4606 CNY
Availability: InStock

Anti-DUBA antibody

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(5 Publications)

Rabbit Polyclonal DUBA antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human OTUD5 aa 150-500.

查看别名

OTU domain-containing protein 5, Deubiquitinating enzyme A, DUBA, OTUD5

2 Images

Supplier Data

Immunoprecipitation - Anti-DUBA antibody (AB176727)

Western blot of immunoprecipitate of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), labeling DUBA using ab176727 used for WB at 1 μg/ml and used for IP at 3 μg/mg lysate, using chemiluminescence with exposure times of 10 seconds.

All lanes:

Immunoprecipitation - Anti-DUBA antibody (ab176727)

Predicted band size: 61 kDa

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Supplier Data

Western blot - Anti-DUBA antibody (AB176727)

Chemiluminescence with exposure times of 3 minutes.

All lanes:

Western blot - Anti-DUBA antibody (ab176727) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Lane 5:

NIH3T3 whole cell lysate at 50 µg

Predicted band size: 61 kDa

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关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human

应用

IP, WB

applications

免疫原

Synthetic Peptide within Human OTUD5 aa 150-500. The exact immunogen used to generate this antibody is proprietary information.

Q96G74

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: 99% Tris buffered saline, 0.1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Deubiquitinating enzyme that functions as a negative regulator of the innate immune system (PubMed : 17991829, PubMed : 22245969, PubMed : 23827681, PubMed : 33523931). Has peptidase activity towards 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (PubMed : 22245969). Can also cleave 'Lys-11'-linked ubiquitin chains (in vitro) (PubMed : 22245969). Acts via TRAF3 deubiquitination and subsequent suppression of type I interferon (IFN) production (PubMed : 17991829). Controls neuroectodermal differentiation through cleaving 'Lys-48'-linked ubiquitin chains to counteract degradation of select chromatin regulators such as ARID1A, HDAC2 and HCF1 (PubMed : 33523931). Acts as a positive regulator of mTORC1 and mTORC2 signaling following phosphorylation by MTOR : acts by mediating deubiquitination of BTRC, leading to its stability (PubMed : 33110214).

See full target information OTUD5

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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2406700 PubMed39721018

2024

OTUD5 Protects Dopaminergic Neurons by Promoting the Degradation of α-Synuclein in Parkinson's Disease Model.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaomeng Song,Tengfei Liu,Lu Yu,Qiuran Ji,Xin Guo,Runzhe Zong,Yiquan Li,Gan Huang,Qidi Xue,Qingyi Fu,Bingyu Liu,Yi Zheng,Lin Chen,Chengjiang Gao,Huiqing Liu

Cell death and differentiation 31:753-767 PubMed38605168

2024

OTUD5 promotes the inflammatory immune response by enhancing MyD88 oligomerization and Myddosome formation.

Applications

Unspecified application

Species

Unspecified reactive species

Yaxing Liu,Jiahua Yuan,Yuling Zhang,Fei Qin,Xuemei Bai,Wanwei Sun,Tian Chen,Feng Liu,Yi Zheng,Xiaopeng Qi,Wei Zhao,Bingyu Liu,Chengjiang Gao

Bosnian journal of basic medical sciences 22:901-911 PubMed35765958

2022

Mechanism of OTUD5 in non-small cell lung cancer cell proliferation, invasion, and migration.

Applications

Unspecified application

Species

Unspecified reactive species

Xuebing Li,Baohua Lu,Lina Zhang,Jing Yang,Yurong Cheng,Dong Yan

Cell death and differentiation 28:900-914 PubMed33110214

2020

Deubiquitinase OTUD5 is a positive regulator of mTORC1 and mTORC2 signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Hwa Cho,Kidae Kim,Sung Ah Kim,Sungryul Park,Bi-Oh Park,Jong-Hwan Kim,Seon-Young Kim,Min Jee Kwon,Myeong Hoon Han,Sung Bae Lee,Byoung Chul Park,Sung Goo Park,Jeong-Hoon Kim,Sunhong Kim

The Journal of biological chemistry 292:11804-11814 PubMed28559278

2017

The p90 ribosomal S6 kinase-UBR5 pathway controls Toll-like receptor signaling via miRNA-induced translational inhibition of tumor necrosis factor receptor-associated factor 3.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Hwa Cho,Sung Ah Kim,Yeon-Soo Seo,Sung Goo Park,Byoung Chul Park,Jeong-Hoon Kim,Sunhong Kim

View all publications

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Anti-DUBA抗体

Anti-DUBA antibody

Immunoprecipitation - Anti-DUBA antibody (AB176727)

Western blot - Anti-DUBA antibody (AB176727)