Dual color: FITC荧光+ PE小鼠IgG1monoclonal [HybIgG1] -同型对照(ab1285)
![Immunocytochemistry/ Immunofluorescence - Dual color: FITC + PE Mouse IgG1monoclonal [HybIgG1] - isotype control (ab1285)](https://www.abcam.cn/ps/products/1/ab1285/Images/ab1285-4144-mouse-igg1monoclonal-hybigg1-fitc-phycoerythrin-isotype-control-immunocytochemistry-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Dual color: FITC + PE Mouse IgG1monoclonal [HybIgG1] - isotype control (ab1285)")
概述
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产品名称
Dual color: FITC荧光+ PE小鼠IgG1monoclonal [HybIgG1] -同型对照
参阅全部 Mouse 亚类对照 -
偶联物
Dual color: FITC + PE -
经测试应用
适用于: Flow Cyt, ICC/IFmore details -
常规说明
This product is a mixture of the same clone separately bearing both FITC and PE labels. This product contains sodium azide, which under acid conditions yields hydrazoic acid, a toxic compound. Azide compounds should be diluted with running water before being discarded to avoid deposits in lead or copper plumbing where explosive conditions may develop.This antibody control duo is a direct immunofluorescence reagent used as an isotype (negative) control for flow cytometric immunophenotyping of erythrocyte-lysed whole blood with monoclonal antibodies.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
Preservative: 0.1% Sodium azide
Constituent: 0.5% BSA -
Concentration information loading... -
Isotype control说明
This antibody control duo is a direct immunofluorescence reagent used as an isotype (negative) control for flow cytometric immunophenotyping of erythrocyte-lysed whole blood with monoclonal antibodies. -
克隆
单克隆 -
克隆编号
HybIgG1 -
骨髓瘤
unknown -
同种型
IgG1 -
轻链类型
unknown -
研究领域
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别名
- Mouse Isotype Control
相关产品
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Alternative Versions
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Compatible Secondaries
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
This antibody control duo is used to determine the unstained lymphocyte population and to determine any non-antigen-specific antibody binding on mononuclear cells separated by density gradient or on lysed whole blood. IgG1 (FITC + PE) immunofluorescence analysis can be performed on a flow cytometerequipped with an excitation source of 488nm and fitted with logarithmic amplifiers. Scatter gates are set on the lymphocyte fraction. Proper electronic compensation and filter selections are necessary for three-color analysis. 10µl ofIgG1 (FITC + PE) is sufficient for labelling of 1x106 cells. |
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| ICC/IF |
Use at an assay dependent concentration.
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| 说明 |
|---|
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Flow Cyt
Use at an assay dependent concentration. This antibody control duo is used to determine the unstained lymphocyte population and to determine any non-antigen-specific antibody binding on mononuclear cells separated by density gradient or on lysed whole blood. IgG1 (FITC + PE) immunofluorescence analysis can be performed on a flow cytometerequipped with an excitation source of 488nm and fitted with logarithmic amplifiers. Scatter gates are set on the lymphocyte fraction. Proper electronic compensation and filter selections are necessary for three-color analysis. 10µl ofIgG1 (FITC + PE) is sufficient for labelling of 1x106 cells. |
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ICC/IF
Use at an assay dependent concentration. |
图片
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Immunocytochemistry/ Immunofluorescence - Dual color: FITC + PE Mouse IgG1monoclonal [HybIgG1] - isotype control (ab1285)ICC/IF image of ab2185 stained MCF7 cells. The cells were 100% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2185, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab1285 尚未被引用在任何文献中。