重组Anti-Drosha抗体[EPR23046-123] (ab242147)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23046-123] to Drosha
- Suitable for: Flow Cyt (Intra), ICC/IF, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Drosha抗体[EPR23046-123]
参阅全部 Drosha 一抗 -
描述
兔单克隆抗体[EPR23046-123] to Drosha -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), ICC/IF, WBmore details
不适用于: IHC-P or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HeLa, K562, PC-12, RAW 264.7, C6 and NIH/3T3 whole cell lysates. ICC/IF: RAW 264.7 and NIH/3T3 cells. Flow Cyt (intra): RAW 264.7 and NIH/3T3 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR23046-123 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab242147于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/500.
Not suitable for Human or Rat. |
|
ICC/IF |
1/50.
Not suitable for Human or Rat. |
|
WB |
1/1000. Detects a band of approximately 158 kDa (predicted molecular weight: 159 kDa).
|
说明 |
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Flow Cyt (Intra)
1/500. Not suitable for Human or Rat. |
ICC/IF
1/50. Not suitable for Human or Rat. |
WB
1/1000. Detects a band of approximately 158 kDa (predicted molecular weight: 159 kDa). |
靶标
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功能
Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies. -
组织特异性
Ubiquitous. -
序列相似性
Contains 1 DRBM (double-stranded RNA-binding) domain.
Contains 2 RNase III domains. -
结构域
The 2 RNase III domains form an intramolecular dimer where the domain 1 cuts the 3'strand while the domain 2 cleaves the 5'strand of pri-miRNAs, independently of each other. -
细胞定位
Nucleus. Nucleus > nucleolus. A fraction is translocated to the nucleolus during the S phase of the cell cycle. Localized in GW bodies (GWBs), also known as P-bodies. - Information by UniProt
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数据库链接
- Entrez Gene: 29102 Human
- Entrez Gene: 14000 Mouse
- Entrez Gene: 310159 Rat
- Omim: 608828 Human
- SwissProt: Q9NRR4 Human
- SwissProt: Q5HZJ0 Mouse
- Unigene: 97997 Human
- Unigene: 293142 Mouse
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别名
- DROSHA antibody
- Drosha double stranded RNA specific endoribonuclease antibody
- Drosha ribonuclease type III antibody
see all
图片
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 cells labelling Drosha with ab242147 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) secondary antibody at 1/1000 dilution (Green). Confocal image showing nuclear and weakly cytoplasmic staining in NIH/3T3 cell line. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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All lanes : Anti-Drosha antibody [EPR23046-123] (ab242147) at 1/1000 dilution
Lane 1 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate
Lane 2 : C6 (rat glial tumor glial cell), whole cell lysate
Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 159 kDa
Observed band size: 158 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Weakly reactivity with undetermined proteins in HeLa.
Exposure time: 3 minutes
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All lanes : Anti-Drosha antibody [EPR23046-123] (ab242147) at 1/1000 dilution
Lane 1 : K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate
Lane 2 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
Lane 3 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 159 kDa
Observed band size: 158 kDa why is the actual band size different from the predicted?
Exposure time: 48 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 48 seconds.
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Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labelling Drosha with ab242147 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Raw264.7 cells labelling Drosha with ab242147 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear and weakly cytoplasmic staining in Raw264.7 cell line. ab195889 Anti-alpha Tubulin antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized Raw 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling Drosha with ab242147 at 1/500 (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (1)
ab242147 被引用在 1 文献中.
- Peng YH et al. Micro ribonucleic acid-363 regulates the phosphatidylinositol 3-kinase/threonine protein kinase axis by targeting NOTCH1 and forkhead box C2, leading to hepatic glucose and lipids metabolism disorder in type 2 diabetes mellitus. J Diabetes Investig 13:236-248 (2022). PubMed: 34739190