AB227460

重组Anti-Drosha抗体[EPR12794] - BSA and Azide free

Anti-Drosha antibody [EPR12794] - BSA and Azide free

RabMAb
Recombinant
KO Validated
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(1 Publication)

Rabbit Recombinant Monoclonal Drosha antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication.

查看别名

RN3, RNASE3L, RNASEN, DROSHA, Ribonuclease 3, Protein Drosha, Ribonuclease III, p241, RNase III

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Drosha antibody [EPR12794] - BSA and Azide free (AB227460)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Drosha antibody [EPR12794] - BSA and Azide free (AB227460)

This IHC data was generated using the same anti-Drosha antibody clone, EPR12794, in a different buffer formulation (cat# ab183732).

Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma tissue labeling Drosha with ab183732 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Lab

Western blot - Anti-Drosha antibody [EPR12794] - BSA and Azide free (AB227460)

This data was developed using the same antibody clone in a different buffer formulation (ab183732).

Lanes 1-3 : Merged signal (red and green). Green - ab183732 observed at 159 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab183732 Anti-Drosha antibody [EPR12794] was shown to specifically react with Drosha in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266217 (knockout cell lysate ab257171) was used. Wild-type and Drosha knockout samples were subjected to SDS-PAGE. ab183732 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Drosha antibody [EPR12794] (<a href='/products/primary-antibodies/drosha-antibody-epr12794-ab183732'>ab183732</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

DROSHA knockout HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human DROSHA knockout HEK-293T cell line (<a href='/products/cell-lines/human-drosha-knockout-hek-293t-cell-line-ab266217'>ab266217</a>)

Lane 3:

HeLa cell lysate at 20 µg

Predicted band size: 159 kDa

Observed band size: 159 kDa

false

不同偶联物与剂型 (1)

Unconjugated

Anti-Drosha antibody [EPR12794]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR12794

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-low-endotoxin-azide-free-ab199376'>ab199376</a> - Rabbit monoclonal IgG (Low endotoxin, Azide free), is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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产品详情

ab227460 is the carrier-free version of ab183732.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies. Plays a role in growth homeostasis in response to autophagy in motor neurons (By similarity).

See full target information DROSHA

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Experimental lung research 40:354-66 PubMed25058539

2014

Knockdown of Drosha in human alveolar type II cells alters expression of SP-A in culture: a pilot study.

Applications

Species

Human

Patricia Silveyra,Zissis C Chroneos,Susan L DiAngelo,Neal J Thomas,Georgios T Noutsios,Nikolaos Tsotakos,Judie A Howrylak,Todd M Umstead,Joanna Floros

View all publications

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重组Anti-Drosha抗体[EPR12794] - BSA and Azide free