Anti-Drebrin抗体(ab11068)
Key features and details
- Rabbit polyclonal to Drebrin
- Suitable for: ICC, WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Chicken, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Drebrin抗体
参阅全部 Drebrin 一抗 -
描述
兔多克隆抗体to Drebrin -
宿主
Rabbit -
经测试应用
适用于: ICC, WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Chicken, Human -
免疫原
Synthetic peptide conjugated to KLH, corresponding to amino acids 22-42 of Human Drebrin.
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常规说明
Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
纯度
IgG fraction -
纯化说明
Whole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab11068于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC | (1) |
Use at an assay dependent concentration.
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| WB |
Use at an assay dependent concentration. Predicted molecular weight: 71 kDa.
ab11068 recognizes drebrin E/A isoforms (95 -120 kDa) and s-drebrin/A2 (approximately 45 kDa) by immunoblotting. An additional unknown band of 20 kDa may be observed in some preparations. |
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| IHC-P | (4) |
Use at an assay dependent concentration.
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| ICC/IF |
Use a concentration of 1 µg/ml.
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| 说明 |
|---|
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ICC
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 71 kDa. ab11068 recognizes drebrin E/A isoforms (95 -120 kDa) and s-drebrin/A2 (approximately 45 kDa) by immunoblotting. An additional unknown band of 20 kDa may be observed in some preparations. |
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IHC-P
Use at an assay dependent concentration. |
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ICC/IF
Use a concentration of 1 µg/ml. |
靶标
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功能
Drebrins might play some role in cell migration, extension of neuronal processes and plasticity of dendrites, respectively. -
组织特异性
Brain neurons. Also found in the heart, placenta, skeletal muscle, kidney and pancreas. -
序列相似性
Contains 1 ADF-H domain. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 396496 Chicken
- Entrez Gene: 1627 Human
- Entrez Gene: 56320 Mouse
- Entrez Gene: 81653 Rat
- Omim: 126660 Human
- SwissProt: P18302 Chicken
- SwissProt: Q16643 Human
- SwissProt: Q9QXS6 Mouse
see all -
别名
- D0S117E antibody
- DBN 1 antibody
- DBN1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Drebrin antibody (ab11068)This image is courtesy of Carl Hobbs, King's College London, United KingdomRabbit polyclonal to Drebrin (ab11068) used in IHC(Formalin/PFA-fixed paraffin-embedded sections; Standard stABCpx-DAB detection). ab11068 detecting Drebrin protein on Rat spinal cord E16 embryo sections fixed in formaldehyde. Antigen retrieval step: Heat mediated. 1% BSA as blocking agent for 10 mins at RT. Ab11068 was used at a dilution of 1/2500 incubated for 16 hours. Secondary Antibody is biotin labeled anti rabbit IgG (1/300). Drebrin positivity conformed to published localisation: essentially, many non-neuronal developing tissues were positive, eg: skeletal primary myotubes. This picture is of spinal cord floor plate region (x20). Nuclei were counterstained using Mayer's Haemalum.
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Immunocytochemistry/ Immunofluorescence - Anti-Drebrin antibody (ab11068)This image is a courtesy of Anonymous Abreviewab11068 staining Drebrin in mouse COR-1 cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with Triton x100 and blocking with 2% BSA was done at 250C for 15 minutes. Samples were incubated with primary antibody (1/100: in 1% BSA, 0.25% gelatin in PBS) for 3 hours at 25°C. An Alexa Fluor®488-conjugated goat polyclonal to rabbit IgG was used at dilution at 1/200 as secondary antibody.
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Immunocytochemistry/ Immunofluorescence - Anti-Drebrin antibody (ab11068)ICC/IF image of ab11068 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11068, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (26)
ab11068 被引用在 26 文献中.
- Chen C et al. Neuronal paxillin and drebrin mediate BDNF-induced force transduction and growth cone turning in a soft-tissue-like environment. Cell Rep 40:111188 (2022). PubMed: 35977504
- Agarwal S et al. Isoflurane Disrupts Postsynaptic Density-95 Protein Interactions Causing Neuronal Synapse Loss and Cognitive Impairment in Juvenile Mice via Canonical NO-mediated Protein Kinase-G Signaling. Anesthesiology 137:212-231 (2022). PubMed: 35504002
- Freitas RO et al. Nano-Infrared Imaging of Primary Neurons. Cells 10:N/A (2021). PubMed: 34685539
- Kushwaha R et al. Non-cell autonomous astrocyte-mediated neuronal toxicity in prion diseases. Acta Neuropathol Commun 9:22 (2021). PubMed: 33546775
- Xu MS et al. Cerebral Ischemia-Reperfusion Is Associated With Upregulation of Cofilin-1 in the Motor Cortex. Front Cell Dev Biol 9:634347 (2021). PubMed: 33777942