Anti-DR5抗体(ab8416)
Key features and details
- Rabbit polyclonal to DR5
- Suitable for: IHC-P, WB, ICC
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-DR5抗体
参阅全部 DR5 一抗 -
描述
兔多克隆抗体to DR5 -
宿主
Rabbit -
特异性
Does not cross react with DR4. -
经测试应用
适用于: IHC-P, WB, ICCmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
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阳性对照
- WB: HEK293, A431, A549, Caco-2, Daudi, HeLa, HepG2, K562, MCF7, Jurkat, SK-N-SH and THP-1, 3T3/NIH, C2-C12 cell lysates (Human, Mouse, Rat). IHC-P: Mouse kidney tissue and rat brain tissue. ICC: HeLa cells.
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常规说明
For information on sample preparation for western blot, please refer here.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading...
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纯度
Affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
应用 | Ab评论 | 说明 |
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IHC-P | (1) |
Use a concentration of 5 µg/ml.
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WB | (3) |
Use a concentration of 2 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 47.8 kDa).
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ICC |
Use a concentration of 5 µg/ml.
|
说明 |
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IHC-P
Use a concentration of 5 µg/ml. |
WB
Use a concentration of 2 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 47.8 kDa). |
ICC
Use a concentration of 5 µg/ml. |
靶标
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功能
Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B. Essential for ER stress-induced apoptosis. -
组织特异性
Widely expressed in adult and fetal tissues; very highly expressed in tumor cell lines such as HeLaS3, K-562, HL-60, SW480, A-549 and G-361; highly expressed in heart, peripheral blood lymphocytes, liver, pancreas, spleen, thymus, prostate, ovary, uterus, placenta, testis, esophagus, stomach and throughout the intestinal tract; not detectable in brain. -
疾病相关
Squamous cell carcinoma of the head and neck -
序列相似性
Contains 1 death domain.
Contains 3 TNFR-Cys repeats. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 8795 Human
- Entrez Gene: 21933 Mouse
- Entrez Gene: 364420 Rat
- Omim: 603612 Human
- SwissProt: O14763 Human
- SwissProt: Q9QZM4 Mouse
- Unigene: 521456 Human
- Unigene: 661668 Human
see all -
别名
- Fas like protein antibody
- Apoptosis inducing protein TRICK2A/2B antibody
- Apoptosis inducing receptor TRAIL R2 antibody
see all
图片
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All lanes : Anti-DR5 antibody (ab8416) at 0.5 µg/ml
Lane 1 : HEK293 cell lysate
Lane 2 : A431 cell lysate
Lane 3 : A549 cell lysate
Lane 4 : Caco-2 cell lysate
Lane 5 : Daudi cell lysate
Lane 6 : HeLa cell lysate
Lane 7 : HepG2 cell lysate
Lane 8 : K562 cell lysate
Lane 9 : MCF7 cell lysate
Lane 10 : Jurkat cell lysate
Lane 11 : SK-N-SH cell lysate
Lane 12 : THP-1 cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 47.8 kDa -
All lanes : Anti-DR5 antibody (ab8416) at 1 µg
Lane 1 : 3T3/NIH cell lysate
Lane 2 : C2C12 cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 47.8 kDa -
Anti-DR5 antibody (ab8416) at 1 µg/ml + Rat Skeletal Muscle at 15 µg
Secondary
Goat Anti-Rabbit IgG HRP Conjugate at 1/10000 dilution
Predicted band size: 47.8 kDaBlocking buffer: 5% NFDM/TBST.
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Immunohistochemical analysis of 4% paraformaldehydefixed mouse kidney tissue using anti-DR5 antibody (ab8416) at 20µg/mL followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution.
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Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-DR5 antibody (ab8416) at 5µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation (microwave for 20 minutes) with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary at room temperature for 1 hour. Counter stained with Hematoxylin.
Endogenous peroxidase blocking: 3% H202 at room temperature for 10 minutes.
Washing: PBS, 2 x 5 minutes.
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IHC-P of 4% paraformaldehyde-fixed rat brain tissue labelled DR5 with ab8416 at 5µg/ml, followed by Goat Anti-Rabbit IgG secondary antibody at 1/5000 dilution (green) and DAPI (blue).
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ICC analysis of 4% paraformaldehyde-fixed (room temperature for 10 minutes) HeLa cells labeling DR5 with ab8416 at 20µg/mL, followed by goat anti-rabbit IgG antibody (Alexa Fluor 488) at 1/500 dilution (green).
Blocking condition: 5% BSA, room temperature for 1 hour.
Primary antibody incubation: 4°C overnight.
Secondary antibody incubation: room temperature for 1 hour.
Washing condition: PBS, 3 x 3 minutes
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ab8416 at 5µg/ml staining DR5 in Hela cells by ICC. Cells were fixed with formaldehyde and blocked with 10% serum for 1 hour at room termperature. Antigen retrieval was by heat mediation with a Citrate buffer (pH 6). Overnight primary antibody at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (56)
ab8416 被引用在 56 文献中.
- Tseng JC et al. Induction of Immune Responses and Phosphatidylserine Exposure by TLR9 Activation Results in a Cooperative Antitumor Effect with a Phosphatidylserine-targeting Prodrug. Int J Biol Sci 19:2648-2662 (2023). PubMed: 37324949
- Zhao D et al. Blocking TRAIL-DR5 signaling pathway with soluble death receptor 5 fusion protein mitigates radiation-induced injury. Front Pharmacol 14:1171293 (2023). PubMed: 37274104
- Chai X et al. Inhibition of HIST1H2AB suppresses the proliferation of lung adenocarcinoma. J Gene Med 25:e3469 (2023). PubMed: 36511295
- Zhang Y et al. THADA inhibition in mice protects against type 2 diabetes mellitus by improving pancreatic β-cell function and preserving β-cell mass. Nat Commun 14:1020 (2023). PubMed: 36823211
- Quiroz-Reyes AG et al. Oxaliplatin Enhances the Apoptotic Effect of Mesenchymal Stem Cells, Delivering Soluble TRAIL in Chemoresistant Colorectal Cancer. Pharmaceuticals (Basel) 16:N/A (2023). PubMed: 37895919