JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB126352

Anti-DPY30抗体

Anti-DPY30 antibody

4

(1 Review)

|

(5 Publications)

Rabbit Polyclonal DPY30 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DPY30.

查看别名

Protein dpy-30 homolog, Dpy-30-like protein, Dpy-30L, DPY30

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-DPY30 antibody (AB126352)
  • ICC/IF

AbReview37834****

Immunocytochemistry/ Immunofluorescence - Anti-DPY30 antibody (AB126352)

ab126352 staining DPY30 in Mouse NIH/3T3 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.2% Tween 20 in PBS and blocked with 3% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (2μg/ml in 3% BSA + 0.2% Tween 20 + PBS) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal (4μg/ml in blocking solution for 1 hour) was used as the secondary antibody.

This image is courtesy of an anonymous Abreview

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPY30 antibody (AB126352)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPY30 antibody (AB126352)

ab126352 at a 1/200 dilution staining DPY30 in paraffin embedded human bronchus tissue by Imunohistochemistry.

Immunocytochemistry/ Immunofluorescence - Anti-DPY30 antibody (AB126352)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-DPY30 antibody (AB126352)

ab126352 at 4 μg/ml staining DPY30 in in PFA/Triton X-100 fixed/permeabilized U251 cells by Immunofluorescence (green).

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

ICC/IF, IHC-P

applications

免疫原

Recombinant Fragment Protein within Human DPY30. The exact immunogen used to generate this antibody is proprietary information.

Q9C005

style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200.00000 - 1/500.00000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25000-2.00000 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
style
left-column
style
left-column
style
left-column

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
存储溶液
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
style
left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

DPY30 also known as Dpy-30-like protein or DPy-30 homolog is a component of the MLL/SET1 histone methyltransferase complexes implicated in histone H3 lysine 4 (H3K4) methylation. This protein has an estimated molecular mass of approximately 9 kDa. It is widely expressed in different tissues which indicates its importance across various cellular environments. The primary role of DPY30 involves facilitating the catalytic activity within these complexes influencing the transcriptional states by modulating chromatin structure.
Biological function summary

DPY30 functions as a core component of the SET1A and SET1B complexes participating in the regulation of gene expression by promoting the trimethylation of H3K4. This histone modification is associated with transcriptionally active chromatin influencing gene activation. DPY30 cooperates with other components like RBBP5 and WDR5 to achieve this modification. By modulating gene expression DPY30 is critical in processes like differentiation and development.

Pathways

DPY30 is deeply involved in chromatin modification and gene transcription pathways. It plays a significant role in the Wnt signaling pathway known for its involvement in developmental processes and cell growth. DPY30 interacts with related proteins within these pathways such as ASH2L which functions as another component of the SET1/MLL complexes to exert its effects on chromatin dynamics and transcription regulation.

DPY30 has been linked to certain cancers particularly acute myeloid leukemia (AML). Research indicates that abnormal activity of the SET1/MLL complexes possibly involving DPY30 may contribute to the leukemogenic process. Additionally it has connections to developmental disorders where mutations impacting histone modification processes disrupt normal developmental signaling pathways. The connection to proteins like MEN1 which interacts with histone modification complexes highlights its role in disease progression.
style
left-column
style
left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

靶点信息

As part of the MLL1/MLL complex, involved in the methylation of histone H3 at 'Lys-4', particularly trimethylation. Histone H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. May play some role in histone H3 acetylation. In a teratocarcinoma cell, plays a crucial role in retinoic acid-induced differentiation along the neural lineage, regulating gene induction and H3 'Lys-4' methylation at key developmental loci. May also play an indirect or direct role in endosomal transport.
See full target information DPY30
style
left-column

文献 (5)

Recent publications for all applications. Explore the full list and refine your search

International journal of medical sciences 20:901-917 PubMed37324189

2023

DPY30 Promotes Proliferation and Cell Cycle Progression of Colorectal Cancer Cells via Mediating H3K4 Trimethylation.

Applications

Unspecified application

Species

Unspecified reactive species

Wei-Chao Su,Xiao-Mei Mao,Si-Yang Li,Chun-Ying Luo,Rui Fan,Hai-Feng Jiang,Lin-Jun Zhang,Ya-Tao Wang,Guo-Qiang Su,Dong-Yan Shen

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 34:12634-12645 PubMed32716582

2020

Single-cell RNA sequencing reveals the landscape of early female germ cell development.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng-Hui Zhao,Jun-Yu Ma,Tie-Gang Meng,Zhen-Bo Wang,Wei Yue,Qian Zhou,Sen Li,Xie Feng,Yi Hou,Heide Schatten,Xiang-Hong Ou,Qing-Yuan Sun

Cell reports 28:1830-1844.e6 PubMed31412250

2019

TrxG Complex Catalytic and Non-catalytic Activity Play Distinct Roles in Pancreas Progenitor Specification and Differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Stephanie A Campbell,Cassandra L McDonald,Nicole A J Krentz,Francis C Lynn,Brad G Hoffman

Biology open 5:1449-1460 PubMed27744293

2016

Regulation of DNA replication and chromosomal polyploidy by the MLL-WDR5-RBBP5 methyltransferases.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Lu,Xiaojun Wu,Feng Yin,Christina Chia-Fang Lee,Min Yu,Ivailo S Mihaylov,Jiekai Yu,Hong Sun,Hui Zhang

Molecular therapy. Nucleic acids 4:e226 PubMed25668340

2015

Histone-targeted Polyplexes Avoid Endosomal Escape and Enter the Nucleus During Postmitotic Redistribution of ER Membranes.

Applications

Unspecified application

Species

Unspecified reactive species

Nikki L Ross,Erik V Munsell,Chandran Sabanayagam,Millicent O Sullivan
View all publications
style
left-column
style
left-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。
style
left-column
style
right-column

关联产品

Select an associated product type
Alternative Product
Primary Antibodies

AB187690

Anti-DPY30 antibody - N-terminal

primary-antibodies

dpy30-antibody-n-terminal-ab187690

0

(0 reviews)

style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com