Anti-DPP9抗体- Catalytic domain (ab42080)
Key features and details
- Rabbit polyclonal to DPP9 - Catalytic domain
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Cow, Human
- Isotype: IgG
概述
-
产品名称
Anti-DPP9抗体- Catalytic domain
参阅全部 DPP9 一抗 -
描述
兔多克隆抗体to DPP9 - Catalytic domain -
宿主
Rabbit -
特异性
This antibody recognises all three forms of DPP9. This antibody does not recognise other DPP family members. -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Cow, Human -
免疫原
Synthetic peptide corresponding to Human DPP9.
(Peptide available asab44599) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 2.9% Sodium chloride -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab42080于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (1) |
Use a concentration of 1 µg/ml.
|
| WB | (3) |
1/1000 - 1/5000. Predicted molecular weight: 98 kDa. Recommended dilution 1/1000 with colourimetric substrates, 1/5000 dilution with chemiluminescent substrates. Dilution optimised using Chromogenic detection.
|
| IHC-P | (3) |
1/50.
|
| 说明 |
|---|
|
ICC/IF
Use a concentration of 1 µg/ml. |
|
WB
1/1000 - 1/5000. Predicted molecular weight: 98 kDa. Recommended dilution 1/1000 with colourimetric substrates, 1/5000 dilution with chemiluminescent substrates. Dilution optimised using Chromogenic detection. |
|
IHC-P
1/50. |
靶标
-
功能
Dipeptidyl peptidase that cleaves off N-terminal dipeptides from proteins having a Pro or Ala residue at position 2. -
组织特异性
Ubiquitously expressed, with highest levels in liver, heart and muscle, and lowest levels in brain. -
序列相似性
Belongs to the peptidase S9B family. DPPIV subfamily. -
细胞定位
Cytoplasm > cytosol. - Information by UniProt
-
数据库链接
- Entrez Gene: 521100 Cow
- Entrez Gene: 91039 Human
- Omim: 608258 Human
- SwissProt: Q86TI2 Human
- Unigene: 515081 Human
-
别名
- Dipeptidyl peptidase 9 antibody
- Dipeptidyl peptidase IV related protein 2 antibody
- Dipeptidyl peptidase IV-related protein 2 antibody
see all
图片
-
Western blot - Anti-DPP9 antibody - Catalytic domain (ab42080)All lanes : Anti-DPP9 antibody - Catalytic domain (ab42080) at 1/1000 dilution
Lane 1 : Cell media from human prostate cancer DU145 cells (treated with SF)
Lane 2 : Cell media from human prostate cancer DU145 cells (treated with RA)
Lane 3 : Cell media from human prostate cancer DU145 cells (treated with IL1-beta)
Predicted band size: 98 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPP9 antibody - Catalytic domain (ab42080)Ab42080 staining Human normal placenta. Staining is localised to the membrane and cytoplasm.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPP9 antibody - Catalytic domain (ab42080)This image is a courtesy of an Abreview submitted by Veronique Duboisab42080 staining DPP9 in bovine testicular tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent paraformaldehyde fixation before heat mediated antigen retrieval in Tris/EDTA buffer pH 9.0 and then the sample was blocked with 3% hydrogen peroxide in TBS for 10 minutes at 25°C. The sections were treated for 10 min with 3% H2O2 in TBS to block endogenous peroxidase activity and incubated subsequently for 30 min with 10% nonimmune goat serum to minimize nonspecific antibody binding. The primary antibody (1/50) was incubated with sample in TBS, 0.3% Triton X-100, 0.1 % BSA for 16 hours at 4°C. A Biotin conjugated goat polyclonal to rabbit IgG was used at dilution 1/200 as secondary antibody.
-
Immunocytochemistry/ Immunofluorescence - Anti-DPP9 antibody - Catalytic domain (ab42080)ICC/IF image of ab42080 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab42080, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
数据表及文件
-
SDS download
-
Datasheet download
文献 (26)
ab42080 被引用在 26 文献中.
- Liu HF et al. Dipeptidyl peptidase-8 induces sorafenib resistance via binding with c-Rel to mediate NF-κB signaling in hepatocellular carcinoma. Cell Biol Int 46:213-221 (2022). PubMed: 34719075
- Hsiao JC et al. A ubiquitin-independent proteasome pathway controls activation of the CARD8 inflammasome. J Biol Chem 298:102032 (2022). PubMed: 35580636
- Vukelic I et al. CD26 Deficiency Controls Macrophage Polarization Markers and Signal Transducers during Colitis Development and Resolution. Int J Mol Sci 23:N/A (2022). PubMed: 35628317
- Ferrara F et al. Ubiquitination as a key regulatory mechanism for O3-induced cutaneous redox inflammasome activation. Redox Biol 56:102440 (2022). PubMed: 36027676
- Moecking J et al. Inflammasome sensor NLRP1 disease variant M1184V promotes autoproteolysis and DPP9 complex formation by stabilizing the FIIND domain. J Biol Chem 298:102645 (2022). PubMed: 36309085