重组Anti-DPP3抗体[EPR9020(B)] (ab133671)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9020(B)] to DPP3
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-DPP3抗体[EPR9020(B)]
参阅全部 DPP3 一抗 -
描述
兔单克隆抗体[EPR9020(B)] to DPP3 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt (Intra),ICC/IF,IHC-P or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human fetal liver lysate, HeLa, 293T and U87 MG cell lysate, THP-1, U-937 and HCT 116 whole cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR9020(B) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133671于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/10000 - 1/50000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
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说明 |
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WB
1/10000 - 1/50000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa). |
靶标
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功能
Cleaves Arg-Arg-beta-naphthylamide. -
序列相似性
Belongs to the peptidase M49 family. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 10072 Human
- Omim: 606818 Human
- SwissProt: Q9NY33 Human
- Unigene: 502914 Human
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别名
- Dipeptidyl aminopeptidase III antibody
- Dipeptidyl arylamidase III antibody
- Dipeptidyl peptidase 3 antibody
see all
图片
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All lanes : Anti-DPP3 antibody [EPR9020(B)] (ab133671) at 1/1000 dilution
Lane 1 : THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg
Lane 2 : U-937 (human histiocytic lymphoma monocyte) whole cell lysate at 20 µg
Lane 3 : HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate at 40 µg
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 83 kDa
Observed band size: 82 kDa why is the actual band size different from the predicted?Blocking and diluting buffer concentrations: 5% NFDM/TBST.
Exposure time: Lane 1: 8 seconds, Lanes 2-3: 6 seconds.
In lane 1, to minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.
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All lanes : Anti-DPP3 antibody [EPR9020(B)] (ab133671) at 1/10000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lane 4 : U87 MG cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 83 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab133671 被引用在 1 文献中.
- Lu K et al. NRF2 Induction Supporting Breast Cancer Cell Survival Is Enabled by Oxidative Stress-Induced DPP3-KEAP1 Interaction. Cancer Res 77:2881-2892 (2017). PubMed: 28416489