Anti-Dopamine Transporter抗体(ab130140)
Key features and details
- Goat polyclonal to Dopamine Transporter
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Dopamine Transporter抗体
参阅全部 Dopamine Transporter 一抗 -
描述
山羊多克隆抗体to Dopamine Transporter -
宿主
Goat -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Chimpanzee, Orangutan -
免疫原
Synthetic peptide:
C-QLTSSTLTNPRQSP
(with a Cysteine residue linker), corresponding to internal sequence amino acids 41-54 of Human Dopamine Transporter (NP_001035.1; Q01959). -
阳性对照
- Human Brain (Substantia nigra) lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab130140 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab130140于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 68 kDa).
1 hour primary incubation is recommended for this product. |
说明 |
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WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 68 kDa). 1 hour primary incubation is recommended for this product. |
靶标
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功能
Amine transporter. Terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals. -
疾病相关
Defects in SLC6A3 are the cause of dystonia-parkinsonism infantile (DYTPRI) [MIM:613135]. It is a neurodegenerative disorder characterized by infantile onset of parkinsonism and dystonia. Other neurologic features include global developmental delay, bradikinesia and pyramidal tract signs. -
序列相似性
Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A3 subfamily. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 6531 Human
- Omim: 126455 Human
- SwissProt: Q01959 Human
- Unigene: 406 Human
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别名
- DA transporter antibody
- DAT 1 antibody
- DAT antibody
see all
图片
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Anti-Dopamine Transporter antibody (ab130140) at 1 µg/ml + Human Substantia nigra lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 68 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. The observed molecular weight corresponds to earlier findings in literature with different antibodies (Mazei-Robison et al, J Neurosci. 2008 Jul 9;28(28):7040-6. PMID: 18614672).
数据表及文件
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Datasheet download
文献 (0)
ab130140 尚未被引用在任何文献中。