重组Anti-Dopamine Receptor D1抗体[EPR24102-105]

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [EPR24102-105] (AB279713)

Immunohistochemical analysis of paraffin-embedded Human corpus striatum tissue labeling Dopamine Receptor D1 with ab279713 at 1/500 (1.026 μg/ml) dilution followed by LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining is on the corpus striatum of human brain. The section was incubated with ab279713 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-Dopamine Receptor D1 antibody [EPR24102-105] (AB279713)

IHC image of Dopamine Receptor D1 staining in a section of frozen normal human thalamus* performed on a Leica BOND™ system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining.

The section was incubated with ab279713, 10ugml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-Dopamine Receptor D1 antibody [EPR24102-105] (AB279713)

IHC image of Dopamine Receptor D1 staining in a section of frozen normal human striatum* performed on a Leica BOND™ system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining.

The section was incubated with ab279713, 10ugml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-Dopamine Receptor D1 antibody [EPR24102-105] (AB279713)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton-X-100 permeabilized frozen rat striatum (fresh) tissue labeling Dopamine Receptor D1 with ab279713 at 1/100 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed (green) at a 1/1000 dilution. The nuclear counterstain is DAPI (blue).
Positive staining on the rat striatum is observed.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at a 1/1000 dilution.

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-Dopamine Receptor D1 antibody [EPR24102-105] (AB279713)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton-X-100 permeabilized frozen rat kidney (fresh) tissue labeling Dopamine Receptor D1 with ab279713 at 1/100 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed (green) at a 1/1000 dilution. The nuclear counterstain is DAPI (blue).
No staining on the rat kidney is observed.
Negative control : kidney (PMID : 2144334).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at a 1/1000 dilution.

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-Dopamine Receptor D1 antibody [EPR24102-105] (AB279713)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton-X-100 permeabilized frozen mouse striatum (fresh) tissue labeling Dopamine Receptor D1 with ab279713 at 1/100 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed (green) at a 1/1000 dilution. The nuclear counterstain is DAPI (blue).
Positive staining on the mouse striatum is observed.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at a 1/1000 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [EPR24102-105] (AB279713)

Immunohistochemical analysis of paraffin-embedded Mouse corpus striatum tissue labeling Dopamine Receptor D1 with ab279713 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the corpus striatum of mouse brain. The section was incubated with ab279713 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [EPR24102-105] (AB279713)

Immunohistochemical analysis of paraffin-embedded Rat corpus striatum tissue labeling Dopamine Receptor D1 with ab279713 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat corpus striatum of mouse brain. The section was incubated with ab279713 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [EPR24102-105] (AB279713)

Immunohistochemical analysis of paraffin-embedded Mouse brain tissue labeling Dopamine Receptor D1 with ab279713 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining is mainly on the corpus striatum of mouse brain. The section was incubated with ab279713 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-Dopamine Receptor D1 antibody [EPR24102-105] (AB279713)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton-X-100 permeabilized frozen mouse kidney (fresh) tissue labeling Dopamine Receptor D1 with ab279713 at 1/100 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed (green) at a 1/1000 dilution. The nuclear counterstain is DAPI (blue).
No staining on the mouse kidney is observed.
Negative control : kidney (PMID : 2144334).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at a 1/1000 dilution.

• WB

Supplier Data

Western blot - Anti-Dopamine Receptor D1 antibody [EPR24102-105] (AB279713)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure time : Lane 1-3 : 15 seconds Lane 4-6 : 69 seconds.

The molecular weight observed is consistent with what have been described in literature (PMID : 28316027).

Negative control : heart, kidney (PMID : 2144334)

Samples are non-boiled as boiling may cause protein aggregates.

All lanes:

Western blot - Anti-Dopamine Receptor D1 antibody [EPR24102-105] (ab279713) at 1/1000 dilution

Lane 1:

Mouse striatum tissue lysate at 20 µg

Lane 2:

Mouse heart tissue lysate at 20 µg

Lane 3:

Mouse kidney tissue lysate at 20 µg

Lane 4:

Rat striatum tissue lysate at 20 µg

Lane 5:

Rat heart tissue lysate at 20 µg

Lane 6:

Rat kidney tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 49 kDa

Observed band size: 95-100 kDa

false

• WB

Lab

Western blot - Anti-Dopamine Receptor D1 antibody [EPR24102-105] (AB279713)

This blot was produced using 4-20% SDS-PAGE containing 15 μg of mouse striatum lysate per lane at 150V for 1hr before being transferred onto a 0.45 μm PVDF membrane at 75V for 1hr. The membrane was then blocked for 1hr using 5% NFDM/TBST, then incubated with ab279713 (1/1000) at room temperature for 1hr. After being washed three times in TBST, the membrane was incubated with Peroxidase conjugated goat anti-rabbit IgG (H+L) (ab97051) at 1/50,000 dilution for 1hr at room temperature. The membrane was washed three times again. Then the signal was developed using the ECL technique.

ab279713 was stored at a range of temperatures (+4°C, +22°C, +37°C) for 1 week before being tested in WB. The image shows the band intensity remains relatively constant across all storage temperatures, demonstrating that antibody activity is not affected under these conditions. This data was generated as part of a structured antibody stability study supporting Abcam’s transition from cold‑chain to ambient shipping.

All lanes:

Western blot - Anti-Dopamine Receptor D1 antibody [EPR24102-105] (ab279713) at 1/1000 dilution

All lanes:

Mouse striatum lysate at 15 µg with NDFM/TBST

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

false

Exposure time: 40s