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Anti-Dopamine Receptor D1抗体(ab40653)

Reviews (2)Q&A (3)References (15)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody (ab40653)
  • Western blot - Anti-Dopamine Receptor D1 antibody (ab40653)

Key features and details

  • Rabbit polyclonal to Dopamine Receptor D1
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Rat, Human
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术，可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-Dopamine Receptor D1抗体
    参阅全部 Dopamine Receptor D1 一抗

  • 描述

    兔多克隆抗体to Dopamine Receptor D1

  • 宿主

    Rabbit

  • 特异性

    This antibody shows reactivity with: D1 Dopamine Receptor (9-21) 100%; D1 Dopamine Receptor 90%; D2 Dopamine Receptor (272-282) 0%; D2 Dopamine Receptor 0%; D3 Dopamine Receptor (2-10) 0%; D3 Dopamine Receptor 0%; D4 Dopamine Receptor (176-185) 0%; D4 Dopamine Receptor 0%; D5 Dopamine Receptor (23-35) 0%; and D5 Dopamine Receptor 0%.

  • 经测试应用

    适用于: ICC/IF, WB, IHC-Pmore details

  • 种属反应性

    与反应: Rat, Human
    预测可用于: Mouse, Cow, Pig, Macaque monkey, Rhesus monkey

  • 免疫原

    Synthetic peptide corresponding to Human Dopamine Receptor D1 aa 1-100 (Cysteine residue).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab40653于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
Use at an assay dependent concentration.
WB
1/1000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa). With this antibody, we have found that blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk.
IHC-P
1/500.
说明
ICC/IF
Use at an assay dependent concentration.
WB
1/1000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa). With this antibody, we have found that blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk.
IHC-P
1/500.

靶标

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody (ab40653)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody (ab40653)
    ab40653 (1:500) staining Dopamine Receptor D1 in human cerebellum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of cytoplasmic and membrane regions in the fibrous glial cells.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • Western blot - Anti-Dopamine Receptor D1 antibody (ab40653)
    Western blot - Anti-Dopamine Receptor D1 antibody (ab40653)
    All lanes : Anti-Dopamine Receptor D1 antibody (ab40653) at 1/1000 dilution

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Rat Cortex Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 49 kDa
    Observed band size: 49 kDa
    Additional bands at: 42 kDa, 75 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 90 seconds

文献 (15)

发表研究结果有使用 ab40653？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab40653 被引用在 15 文献中.

  • Gao S  et al. Striatal D1 Dopamine Neuronal Population Dynamics in a Rat Model of Levodopa-Induced Dyskinesia. Front Aging Neurosci 14:783893 (2022). PubMed: 35185524
  • Agustí A  et al. Bacteroides uniformis CECT 7771 Modulates the Brain Reward Response to Reduce Binge Eating and Anxiety-Like Behavior in Rat. Mol Neurobiol 58:4959-4979 (2021). PubMed: 34228269
  • Felippe RM  et al. Experimental Social Stress: Dopaminergic Receptors, Oxidative Stress, and c-Fos Protein Are Involved in Highly Aggressive Behavior. Front Cell Neurosci 15:696834 (2021). PubMed: 34489642
  • Eom TY  et al. Schizophrenia-related microdeletion causes defective ciliary motility and brain ventricle enlargement via microRNA-dependent mechanisms in mice. Nat Commun 11:912 (2020). PubMed: 32060266
  • Williams MT  et al. Whole brain proton irradiation in adult Sprague Dawley rats produces dose dependent and non-dependent cognitive, behavioral, and dopaminergic effects. Sci Rep 10:21584 (2020). PubMed: 33299021
View all Publications for this product

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1-5 of 5 Abreviews or Q&A

Immunohistochemistry free floating abreview for Anti-Dopamine Receptor D1 antibody

 Poor
Abreviews
abreview image
Application
Immunohistochemistry free floating
Sample
Rat Tissue sections (Rat brain striatum)
Specification
Rat brain striatum
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Apr 09 2019

Western blot

 Inconclusive
Abreviews
abreview image
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (4-12% gradient)
Sample
Mouse Tissue lysate - whole (hypothalamus and spine)
Specification
hypothalamus and spine
Blocking step
normal donkey serum/PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Tatiana Novoselova

Verified customer

提交于 Jun 17 2013

Question

Inquiry: Hi, I need to do preadsorption controls to test the specificity for a range of antibodies. Do you sell peptides for the following: Anti-DCC antibody [AF5] (ab16793) Anti-Dopamine receptor 1 (ab40653) Anti-Dopamine receptor 3 (ab40655). If you do not have the original peptides the antibodies were made against, do you have any peptides matching these receptors I could use? Kind regards,

Read More
Answer

Thank you for your patience.

I am happy to confirm that we were able to add the immunogen peptides for the antibody anti-Dopamine receptor 1 (ab40653) and the anti-Dopamine receptor 3 (ab40655).

The immunogen peptide for ab40653 can be found with the catalog number ab137968 https://www.abcam.com/Dopamine-Receptor-D1-peptide-ab137968.html (or use the following: https://www.abcam.com/Dopamine-Receptor-D1-peptide-ab137968.html).

The immunogen peptide for ab40655 can be found with the catalog number ab137997 https://www.abcam.com/Dopamine-Receptor-D3DRD3-peptide-ab137997.html (or use the following: https://www.abcam.com/Dopamine-Receptor-D3DRD3-peptide-ab137997.html).

I hope the peptides are useful for you. Please do not hesitate to contact us again, should you need any additional information or should youhave any other questions.

I wish you a good weekend!

Read More

Question

Dear tech support! How are you?



Do you have the following items with the corresponding lot# as indicated below in stock?



AB40655; lot#498327

AB40656; lot#549009

AB40653; lot#@731805


I look forward to receiving your response!

Thank you heaps

Kind regards,

Read More
Answer

Thank you for your enquiry.

I am sorry to confirm that regrettablythese lot numbers for these antibodies are no longer available. These are very old lot numbers.

Should you have any further questions, please do not hesitate to contact us.

Read More

Question

I would like to buy an anti-dopamine receptor D1 antibody, and I doubt between the ab20066 and the ab40653. I would like to detect this protein by WB in different rat brain areas. I have been reading and I think that for my experiments it is better if I detect de N-terminus part of the protein, that is why I have choosen between this two antibodies. I would like to know which are the differences between them, as I have seen that ab40653 is linked to a carrier protein by a carboxyl terminal Cysteine residue linker, and I do not know what does this mean. I thank you in advance for your help.

Read More
Answer

Thank you for your inquiry. We would guarantee both ab20066 and ab40653 to work with rat samples in WB in accordance with our Abpromise. https://www.abcam.com/index.html?pageconfig=resource&rid=10372 I wouldn't be too concerned about the linker on the immunogen.  Most customers will choose an antibody based on the data we have on the datasheet.  These antibodies are more or less the same in terms of immunogen, testing information, purity, etc. We have specifically tested both in various regions of PLP fixed Rat brain sections. This includes Medial septum, Nucleus accumbens, Dentate gyrus, Globus pallidus, Medial forebrain bundle, Cortex regions 1-3, Substantia Nigra reticulata, and the Ventral tegmental area.  We have 2 references for ab20066: https://www.abcam.com/Dopamine-Receptor-D1-antibody-ab20066-references.html For ab40653, we do know of a rat-specific reference in WB. https://www.abcam.com/Dopamine-Receptor-D1-antibody-ab40653-references.html Essentially, we would guarantee either to work in the way you are testing, and these antibodies are essentially very similar. I hope this information helps.  Please contact us with any other questions.

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