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Neuroscience Neurotransmitter Biogenic Amines Dopamine

Anti-DOPA Decarboxylase/DDC抗体(ab3905)

  • Datasheet
  • SDS
Reviews (9) Submit a question References (26)

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Western blot - Anti-DOPA Decarboxylase/DDC antibody (ab3905)
  • Western blot - Anti-DOPA Decarboxylase/DDC antibody (ab3905)

Key features and details

  • Rabbit polyclonal to DOPA Decarboxylase/DDC
  • Suitable for: WB
  • Reacts with: Mouse, Rat
  • Isotype: IgG

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概述

  • 产品名称

    Anti-DOPA Decarboxylase/DDC抗体
    参阅全部 DOPA Decarboxylase/DDC 一抗
  • 描述

    兔多克隆抗体to DOPA Decarboxylase/DDC
  • 宿主

    Rabbit
  • 经测试应用

    适用于: WBmore details
  • 种属反应性

    与反应: Mouse, Rat
  • 免疫原

    Recombinant fragment corresponding to Bovine DOPA Decarboxylase/DDC. expressed in E. coli and purified from inclusion bodies

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.50
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 0.316% Tris HCl, 0.05% Tween, 0.238% HEPES, 10% Glycerol, 0.1% BSA
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Neuroscience
    • Neurotransmitter
    • Biogenic Amines
    • Dopamine
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Hormone biosynthesis
    • Metabolism
    • Pathways and Processes
    • Endocrine metabolism
    • Hormone biosynthesis
    • Metabolism
    • Types of disease
    • Cancer

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human DOPA Decarboxylase/DDC protein (ab85332)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab3905于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (1)
Use a concentration of 0.1 - 0.5 µg/ml.
说明
WB
Use a concentration of 0.1 - 0.5 µg/ml.

靶标

  • 功能

    Catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine.
  • 通路

    Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 2/2.
  • 疾病相关

    Defects in DDC are the cause of aromatic L-amino-acid decarboxylase deficiency (AADCD) [MIM:608643]. AADCD deficiency is an inborn error in neurotransmitter metabolism that leads to combined serotonin and catecholamine deficiency. It causes developmental and psychomotor delay, poor feeding, lethargy, ptosis, intermittent hypothermia, gastrointestinal disturbances. The onset is early in infancy and inheritance is autosomal recessive.
  • 序列相似性

    Belongs to the group II decarboxylase family.
  • Target information above from: UniProt accession P20711 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 13195 Mouse
    • Entrez Gene: 24311 Rat
    • SwissProt: O88533 Mouse
    • SwissProt: P14173 Rat
    • Unigene: 12906 Mouse
    • Unigene: 11064 Rat
    • 别名

      • AADC antibody
      • Aromatic L Amino Acid Decarboxylase antibody
      • Aromatic-L-amino-acid decarboxylase antibody
      • DDC antibody
      • DDC_HUMAN antibody
      • DOPA decarboxylase (aromatic L-amino acid decarboxylase) antibody
      • DOPA decarboxylase antibody
      see all

    图片

    • Western blot - Anti-DOPA Decarboxylase/DDC antibody (ab3905)
      Western blot - Anti-DOPA Decarboxylase/DDC antibody (ab3905)
      Anti-DOPA Decarboxylase/DDC antibody (ab3905) + Mouse cerebellum lysate at 0.2 mg/ml

      Performed under reducing conditions.
    • Western blot - Anti-DOPA Decarboxylase/DDC antibody (ab3905)
      Western blot - Anti-DOPA Decarboxylase/DDC antibody (ab3905)
      Anti-DOPA Decarboxylase/DDC antibody (ab3905) + Rat adrenal medulla lysate

    实验方案

    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (26)

    发表研究结果有使用 ab3905?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab3905 被引用在 26 文献中.

    • Raina A  et al. Dopamine promotes the neurodegenerative potential of ß-synuclein. J Neurochem 156:674-691 (2021). PubMed: 32730640
    • Heo JY  et al. Aberrant Tonic Inhibition of Dopaminergic Neuronal Activity Causes Motor Symptoms in Animal Models of Parkinson's Disease. Curr Biol 30:276-291.e9 (2020). PubMed: 31928877
    • Chen X  et al. Entorhinal cortex-based metabolic profiling of chronic restraint stress mice model of depression. Aging (Albany NY) 12:3042-3052 (2020). PubMed: 32074509
    • Kao CY  et al. Elevated COUP-TFII expression in dopaminergic neurons accelerates the progression of Parkinson's disease through mitochondrial dysfunction. PLoS Genet 16:e1008868 (2020). PubMed: 32579581
    • Scott AL  et al. Regulation of catecholamine release from the adrenal medulla is altered in deer mice (Peromyscus maniculatus) native to high altitudes. Am J Physiol Regul Integr Comp Physiol 317:R407-R417 (2019). PubMed: 31242021
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
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    1-9 of 9 Abreviews or Q&A

    Western blot abreview for Anti-DOPA Decarboxylase/DDC antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Brain lysate)
    Gel Running Conditions
    Reduced Denaturing (12% gel)
    Loading amount
    25 µg
    Specification
    Brain lysate
    Blocking step
    BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Oct 16 2018

    Immunohistochemistry (Resin sections) abreview for Anti-DOPA Decarboxylase/DDC antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Resin sections)
    Sample
    Mouse Tissue sections (Retina)
    Specification
    Retina
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jan 26 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-DOPA Decarboxylase/DDC antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Drosophila melanogaster Cell (central nervous system (ventral nerve cord))
    Permeabilization
    Yes - 0.2% Triton-X
    Specification
    central nervous system (ventral nerve cord)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Shinya Yamamoto

    Verified customer

    提交于 Jun 13 2013

    Immunohistochemistry (Frozen sections) abreview for Anti-DOPA Decarboxylase/DDC antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Nov 16 2012

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-DOPA Decarboxylase / DDC antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Midbrain section)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6.0
    Permeabilization
    No
    Specification
    Midbrain section
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 24°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Mar 26 2010

    Immunohistochemistry (Frozen sections) abreview for Anti-DOPA Decarboxylase/DDC antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (Brain)
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Dec 14 2009

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-DOPA Decarboxylase/DDC antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Rat Tissue sections (Brain)
    Antigen retrieval step
    None
    Permeabilization
    Yes - 0.1% triton x-100
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Aug 01 2008

    Immunohistochemistry (Frozen sections) abreview for Anti-DOPA Decarboxylase/DDC antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (young postnatal mouse brain)
    Permeabilization
    Yes - 0.2% Triton X-100
    Specification
    young postnatal mouse brain
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Sep 21 2007

    Immunohistochemistry (Frozen sections) abreview for Anti-DOPA Decarboxylase antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Brain slices (40um thick sections))
    Specification
    Brain slices (40um thick sections)
    Fixative
    Paraformaldehyde
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10%
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Mar 13 2006

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