AB131461

Anti-DOK2 (phospho Y299)抗体

Name: DOK2(磷酸化Y299)抗体 (ab131461)| Abcam中文官网
Brand: Abcam Limited
SKU: AB131461
Price: 4606 CNY
Availability: InStock

Anti-DOK2 (phospho Y299) antibody

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(1 Publication)

Rabbit Polyclonal DOK2 phospho Y299 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication.

查看别名

Docking protein 2, Downstream of tyrosine kinase 2, p56(dok-2), DOK2

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOK2 (phospho Y299) antibody (AB131461)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOK2 (phospho Y299) antibody (AB131461)

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling DOK2 (phospho Y299) with ab131461 at 1/50 dilution. The image on the right is treated with the synthesized peptide.

Immunocytochemistry/ Immunofluorescence - Anti-DOK2 (phospho Y299) antibody (AB131461)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-DOK2 (phospho Y299) antibody (AB131461)

Immunocytochemistry/ Immunofluorescence analysis of methanol-fixed HeLa cells labelling DOK2 (phospho Y299) with ab131461 at 1/100 dilution.

Unknown

Western blot - Anti-DOK2 (phospho Y299) antibody (AB131461)

All lanes:

Western blot - Anti-DOK2 (phospho Y299) antibody (ab131461) at 1/500 dilution

Lane 1:

K562 cell extracts with blocking peptide

Lane 2:

K562 cell extracts

Predicted band size: 45 kDa

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关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, ICC/IF, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/200", "ICCIF-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

ab131461 was purified by using epitope specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.

存储溶液

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

运输条件

Blue Ice

储存信息

Stable for 12 months at -20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

DOK2 also known as Docking Protein 2 or p56dok-2 is an adaptor protein with a molecular weight of approximately 56 kDa. It is expressed in hematopoietic cells and is found in various tissues including the spleen thymus and bone marrow. DOK2 acts mechanically by mediating signal transduction processes and interacting with several receptors particularly those involved in immune response.

Biological function summary

Within the cellular environment DOK2 plays an important role in the negative regulation of cell signaling pathways. It acts as a part of a signaling complex where it inhibits the Ras pathway by interacting with Ras GTPase-activating proteins. This interaction prevents excessive cell proliferation and contributes to maintaining cellular homeostasis. DOK2's inhibitory functions are essential in controlling cell cycle and apoptosis processes.

Pathways

DOK2 operates as a negative regulator in key signaling pathways such as the epidermal growth factor receptor (EGFR) pathway and the T cell receptor (TCR) signaling pathway. In the TCR pathway it associates with proteins like SHIP and SHC thereby playing a part in modulating T cell activation and signal progression. In these pathways DOK2’s presence prevents overactivation that could otherwise lead to pathological conditions.

Aberrant DOK2 function has been linked to the development of certain types of leukemia such as chronic myeloid leukemia (CML) where it may play a role in leukemic cell survival. Altered DOK2 expression also shows connection to autoimmune diseases potentially through dysregulation of immune cell signaling. An interaction with proteins like BCR-ABL in CML illustrates DOK2’s involvement in disease states making it an important focus for research in targeted therapies.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK2 may modulate the cellular proliferation induced by IL-4, as well as IL-2 and IL-3. May be involved in modulating Bcr-Abl signaling. Attenuates EGF-stimulated MAP kinase activation (By similarity).

See full target information Docking protein 2 phospho Y299

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular biochemistry 119:10338-10350 PubMed30171710

2018

Characterization of potential TRPP2 regulating proteins in early Xenopus embryos.

Applications

Unspecified application

Species

Unspecified reactive species

Mélinée Futel,Ronan Le Bouffant,Isabelle Buisson,Muriel Umbhauer,Jean-François Riou

View all publications

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Anti-DOK2 (phospho Y299)抗体

Anti-DOK2 (phospho Y299) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOK2 (phospho Y299) antibody (AB131461)

Immunocytochemistry/ Immunofluorescence - Anti-DOK2 (phospho Y299) antibody (AB131461)

Western blot - Anti-DOK2 (phospho Y299) antibody (AB131461)