Anti-DOK2抗体(ab131488)
Key features and details
- Rabbit polyclonal to DOK2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-DOK2抗体
参阅全部 DOK2 一抗 -
描述
兔多克隆抗体to DOK2 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide conjugated to KLH, surrounding internal sequence amino acids 297-301 (G-E-Y-A-V) of Human DOK2 (NP_003965.2).
-
阳性对照
- Jurkat cell extract; HeLa cells; Human breast carcinoma tissue.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab131488 was purified by affinity chromatography using epitope specific peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131488于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 56 kDa (predicted molecular weight: 45 kDa).
|
|
| IHC-P |
1/50 - 1/100.
|
|
| ICC/IF |
1/100 - 1/200.
|
| 说明 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 56 kDa (predicted molecular weight: 45 kDa). |
|
IHC-P
1/50 - 1/100. |
|
ICC/IF
1/100 - 1/200. |
靶标
-
功能
DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK2 may modulate the cellular proliferation induced by IL-4, as well as IL-2 and IL-3. May be involved in modulating Bcr-Abl signaling. Attenuates EGF-stimulated MAP kinase activation. -
组织特异性
Highly expressed in peripheral blood leukocytes, lymph nodes and spleen. Lower expression in thymus, bone marrow and fetal liver. -
序列相似性
Belongs to the DOK family. Type A subfamily.
Contains 1 IRS-type PTB domain.
Contains 1 PH domain. -
结构域
PTB domain mediates receptor interaction. -
翻译后修饰
On immunoreceptor stimulation, phosphorylated on C-terminal tyrosine residues. Phosphorylation on Tyr-345 is required for binding to the SH2 domain of NCK. Phosphorylation on both Tyr-271 and Tyr-299 is required for interaction with RASGAP. - Information by UniProt
-
数据库链接
- Entrez Gene: 9046 Human
- Omim: 604997 Human
- SwissProt: O60496 Human
- Unigene: 71215 Human
-
别名
- Docking protein 2 56kDa antibody
- Docking protein 2 antibody
- DOK 2 antibody
see all
图片
-
Western blot - Anti-DOK2 antibody (ab131488)Anti-DOK2 antibody (ab131488) at 1/500 dilution + Jurkat cell extract
Predicted band size: 45 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-DOK2 antibody (ab131488)Immunofluorescent analysis of methanol-fixed HeLa cells labelling DOK2 with ab131488 at 1/100 dilution. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOK2 antibody (ab131488)Immunohistochemical analysis of paraffin-embedded Human Breast carcinoma tissue labelling DOK2 with ab131488 at 1/50 dilution. The image on the right is treated with the synthesized peptide.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab131488 尚未被引用在任何文献中。