Anti-DOK1 (phospho Y398)抗体(ab75741)
Key features and details
- Rabbit polyclonal to DOK1 (phospho Y398)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-DOK1 (phospho Y398)抗体
参阅全部 DOK1 一抗 -
描述
兔多克隆抗体to DOK1 (phospho Y398) -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human DOK1 (phospho Y398).
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阳性对照
- Human breast carcinoma tissue. Extracts from K562 cells, starved.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab75741 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab75741于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 52 kDa.
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| IHC-P |
1/50 - 1/100.
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| 说明 |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 52 kDa. |
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IHC-P
1/50 - 1/100. |
靶标
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功能
DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3. -
组织特异性
Expressed in pancreas, heart, leukocyte and spleen. Expressed in both resting and activated peripheral blood T-cells. -
序列相似性
Belongs to the DOK family. Type A subfamily.
Contains 1 IRS-type PTB domain.
Contains 1 PH domain. -
结构域
The PTB domain mediates receptor interaction. -
翻译后修饰
Constitutively tyrosine-phosphorylated.
Phosphorylated on tyrosine residues by the insulin receptor kinase. Results in the negative regulation of the insulin signaling pathway. -
细胞定位
Cytoplasm and Cytoplasm > perinuclear region. - Information by UniProt
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数据库链接
- Entrez Gene: 1796 Human
- Omim: 602919 Human
- SwissProt: Q99704 Human
- Unigene: 103854 Human
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别名
- Docking protein 1 62kD antibody
- Docking protein 1 antibody
- DOK 1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOK1 (phospho Y398) antibody (ab75741)ab75741 at 1/50 dilution staining DOK1 in human breast carcinoma by Immunohistochemistry using paraffin-embedded tissue, in the absence or presence of the immunising phosphopeptide.
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Western blot - Anti-DOK1 (phospho Y398) antibody (ab75741)All lanes : Anti-DOK1 (phospho Y398) antibody (ab75741) at 1/500 dilution
Lane 1 : K562 cell extracts, unstarved
Lane 2 : K562 cell extracts, starved
Predicted band size: 52 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab75741 尚未被引用在任何文献中。