Anti-DOK1抗体(ab8112)
Key features and details
- Rabbit polyclonal to DOK1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-DOK1抗体
参阅全部 DOK1 一抗 -
描述
兔多克隆抗体to DOK1 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human DOK1 aa 400-500.
Database link: Q99704 -
阳性对照
- Jurkat whole cell lysate
-
常规说明
Tyrosine kinase substrate p62DOKSignals from most growth factors and cytokines are transduced by receptor tyrosine kinases or non-receptortyrosine kinases. Activated tyrosine kinases phosphorylatetheir substrates, which mediate the cellular response to extracellular stimuli. A long-sought major substrate termed p62dok (downstream of tyrosine kinase) for many tyrosine kinases including c-kit, v-abl, v-Fps, v-Src, v-Fms, and activated EGF, PDGF, IGF, VEGF and insulin receptors was identified recently from human and mouse by several laboratories. Upon phosphorylation, p62dok forms a complex with the ras GTPase-activating protein (RasGAP). p62dok represents a new family with very recently identified p56 dok.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
纯度
Immunogen affinity purified -
Primary antibody说明
Signals from most growth factors and cytokines are transduced by receptor tyrosine kinases or non-receptortyrosine kinases. Activated tyrosine kinases phosphorylatetheir substrates, which mediate the cellular response to extracellular stimuli. A long-sought major substrate termed p62dok (downstream of tyrosine kinase) for many tyrosine kinases including c-kit, v-abl, v-Fps, v-Src, v-Fms, and activated EGF, PDGF, IGF, VEGF and insulin receptors was identified recently from human and mouse by several laboratories. Upon phosphorylation, p62dok forms a complex with the ras GTPase-activating protein (RasGAP). p62dok represents a new family with very recently identified p56 dok. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 62 kDa.
|
|
| ICC/IF |
Use a concentration of 2 µg/ml.
|
| 说明 |
|---|
|
WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 62 kDa. |
|
ICC/IF
Use a concentration of 2 µg/ml. |
靶标
-
功能
DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3. -
组织特异性
Expressed in pancreas, heart, leukocyte and spleen. Expressed in both resting and activated peripheral blood T-cells. -
序列相似性
Belongs to the DOK family. Type A subfamily.
Contains 1 IRS-type PTB domain.
Contains 1 PH domain. -
结构域
The PTB domain mediates receptor interaction. -
翻译后修饰
Constitutively tyrosine-phosphorylated.
Phosphorylated on tyrosine residues by the insulin receptor kinase. Results in the negative regulation of the insulin signaling pathway. -
细胞定位
Cytoplasm and Cytoplasm > perinuclear region. - Information by UniProt
-
数据库链接
- Entrez Gene: 1796 Human
- Omim: 602919 Human
- SwissProt: Q99704 Human
- Unigene: 103854 Human
-
别名
- Docking protein 1 62kD antibody
- Docking protein 1 antibody
- DOK 1 antibody
see all
图片
-
Western blot - Anti-DOK1 antibody (ab8112)All lanes : Anti-DOK1 antibody (ab8112) at 1 µg/ml
Lane 1 : Jurkat cell lysates
Lane 2 : THP-1 cell lysate
Observed band size: 62 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-DOK1 antibody (ab8112)ab8112 at 2µg/ml staining DOK1 in K562 cells by ICC/IF
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (8)
ab8112 被引用在 8 文献中.
- Sheng L et al. Overexpression of Fc?RIIB regulates downstream protein phosphorylation and suppresses B cell activation to ameliorate systemic lupus erythematosus. Int J Mol Med 46:1409-1422 (2020). PubMed: 32945349
- Friedrich T et al. Subcellular compartmentalization of docking protein-1 contributes to progression in colorectal cancer. EBioMedicine 8:159-72 (2016). IHC . PubMed: 27428427
- Siouda M et al. Epstein-Barr virus down-regulates tumor suppressor DOK1 expression. PLoS Pathog 10:e1004125 (2014). WB . PubMed: 24809689
- Barrett A et al. A crucial role for DOK1 in PDGF-BB-stimulated glioma cell invasion through p130Cas and Rap1 signalling. J Cell Sci 127:2647-58 (2014). PubMed: 24762811
- Celis-Gutierrez J et al. Dok1 and Dok2 proteins regulate natural killer cell development and function. EMBO J 33:1928-40 (2014). PubMed: 24963146
- Siouda M et al. Transcriptional regulation of the human tumor suppressor DOK1 by E2F1. Mol Cell Biol 32:4877-90 (2012). WB ; Human . PubMed: 23028047
- Schoenborn JR et al. Feedback circuits monitor and adjust Basal lck-dependent events in T cell receptor signaling. Sci Signal 4:ra59 (2011). PubMed: 21917715
- Park SK et al. Src-like adaptor protein (SLAP) is upregulated in antigen-stimulated mast cells and acts as a negative regulator. Mol Immunol 46:2133-9 (2009). WB . PubMed: 19371953