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Cardiovascular Blood Platelets

Anti-DOK1抗体(ab8112)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (8)

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Western blot - Anti-DOK1 antibody (ab8112)
  • Immunocytochemistry/ Immunofluorescence - Anti-DOK1 antibody (ab8112)

Key features and details

  • Rabbit polyclonal to DOK1
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-DOK1抗体
    参阅全部 DOK1 一抗
  • 描述

    兔多克隆抗体to DOK1
  • 宿主

    Rabbit
  • 经测试应用

    适用于: WB, ICC/IFmore details
  • 种属反应性

    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human DOK1 aa 400-500.
    Database link: Q99704

  • 阳性对照

    • Jurkat whole cell lysate
  • 常规说明

    Tyrosine kinase substrate p62DOK


    Signals from most growth factors and cytokines are transduced by receptor tyrosine kinases or non-receptortyrosine kinases. Activated tyrosine kinases phosphorylatetheir substrates, which mediate the cellular response to extracellular stimuli. A long-sought major substrate termed p62dok (downstream of tyrosine kinase) for many tyrosine kinases including c-kit, v-abl, v-Fps, v-Src, v-Fms, and activated EGF, PDGF, IGF, VEGF and insulin receptors was identified recently from human and mouse by several laboratories. Upon phosphorylation, p62dok forms a complex with the ras GTPase-activating protein (RasGAP). p62dok represents a new family with very recently identified p56 dok.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液

    pH: 7.2
    Preservative: 0.02% Sodium azide
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • Primary antibody说明

    Signals from most growth factors and cytokines are transduced by receptor tyrosine kinases or non-receptortyrosine kinases. Activated tyrosine kinases phosphorylatetheir substrates, which mediate the cellular response to extracellular stimuli. A long-sought major substrate termed p62dok (downstream of tyrosine kinase) for many tyrosine kinases including c-kit, v-abl, v-Fps, v-Src, v-Fms, and activated EGF, PDGF, IGF, VEGF and insulin receptors was identified recently from human and mouse by several laboratories. Upon phosphorylation, p62dok forms a complex with the ras GTPase-activating protein (RasGAP). p62dok represents a new family with very recently identified p56 dok.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Cardiovascular
    • Blood
    • Platelets
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Other
    • Signal Transduction
    • Adapters
    • Cytoplasmic
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    • Ras Family
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    • Signal transduction
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    • Metabolism
    • Types of disease
    • Obesity

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human DOK1 protein (ab112280)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab8112于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 62 kDa.
ICC/IF
Use a concentration of 2 µg/ml.
说明
WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 62 kDa.
ICC/IF
Use a concentration of 2 µg/ml.

靶标

  • 功能

    DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3.
  • 组织特异性

    Expressed in pancreas, heart, leukocyte and spleen. Expressed in both resting and activated peripheral blood T-cells.
  • 序列相似性

    Belongs to the DOK family. Type A subfamily.
    Contains 1 IRS-type PTB domain.
    Contains 1 PH domain.
  • 结构域

    The PTB domain mediates receptor interaction.
  • 翻译后修饰

    Constitutively tyrosine-phosphorylated.
    Phosphorylated on tyrosine residues by the insulin receptor kinase. Results in the negative regulation of the insulin signaling pathway.
  • 细胞定位

    Cytoplasm and Cytoplasm > perinuclear region.
  • Target information above from: UniProt accession Q99704 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 1796 Human
    • Omim: 602919 Human
    • SwissProt: Q99704 Human
    • Unigene: 103854 Human
    • 别名

      • Docking protein 1 62kD antibody
      • Docking protein 1 antibody
      • DOK 1 antibody
      • DOK1 antibody
      • DOK1_HUMAN antibody
      • Downstream of tyrosine kinase 1 antibody
      • p62(dok) antibody
      • P62DOK antibody
      • pp62 antibody
      see all

    图片

    • Western blot - Anti-DOK1 antibody (ab8112)
      Western blot - Anti-DOK1 antibody (ab8112)
      All lanes : Anti-DOK1 antibody (ab8112) at 1 µg/ml

      Lane 1 : Jurkat cell lysates
      Lane 2 : THP-1 cell lysate

      Observed band size: 62 kDa why is the actual band size different from the predicted?

    • Immunocytochemistry/ Immunofluorescence - Anti-DOK1 antibody (ab8112)
      Immunocytochemistry/ Immunofluorescence - Anti-DOK1 antibody (ab8112)

      ab8112 at 2µg/ml staining DOK1 in K562 cells by ICC/IF

    实验方案

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (8)

    发表研究结果有使用 ab8112?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab8112 被引用在 8 文献中.

    • Sheng L  et al. Overexpression of Fc?RIIB regulates downstream protein phosphorylation and suppresses B cell activation to ameliorate systemic lupus erythematosus. Int J Mol Med 46:1409-1422 (2020). PubMed: 32945349
    • Friedrich T  et al. Subcellular compartmentalization of docking protein-1 contributes to progression in colorectal cancer. EBioMedicine 8:159-72 (2016). IHC . PubMed: 27428427
    • Siouda M  et al. Epstein-Barr virus down-regulates tumor suppressor DOK1 expression. PLoS Pathog 10:e1004125 (2014). WB . PubMed: 24809689
    • Barrett A  et al. A crucial role for DOK1 in PDGF-BB-stimulated glioma cell invasion through p130Cas and Rap1 signalling. J Cell Sci 127:2647-58 (2014). PubMed: 24762811
    • Celis-Gutierrez J  et al. Dok1 and Dok2 proteins regulate natural killer cell development and function. EMBO J 33:1928-40 (2014). PubMed: 24963146
    • Siouda M  et al. Transcriptional regulation of the human tumor suppressor DOK1 by E2F1. Mol Cell Biol 32:4877-90 (2012). WB ; Human . PubMed: 23028047
    • Schoenborn JR  et al. Feedback circuits monitor and adjust Basal lck-dependent events in T cell receptor signaling. Sci Signal 4:ra59 (2011). PubMed: 21917715
    • Park SK  et al. Src-like adaptor protein (SLAP) is upregulated in antigen-stimulated mast cells and acts as a negative regulator. Mol Immunol 46:2133-9 (2009). WB . PubMed: 19371953

    客户评价及客户问答

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    Immunoprecipitation abreview for Anti-DOK1 antibody

    Excellent
    Abreviews
    Abreviews
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (Jurkat cells)
    Total protein in input
    2e+007 cells
    Specification
    Jurkat cells
    Immuno-precipitation step
    Protein A
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Mr. Guittard Geoffrey

    Verified customer

    提交于 Jul 27 2006

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