JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB254866

Anti-DOHH抗体

Anti-DOHH antibody

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Polyclonal DOHH antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human DOHH aa 1-150.

查看别名

HLRC1, DOHH, Deoxyhypusine hydroxylase, hDOHH, Deoxyhypusine dioxygenase, Deoxyhypusine monooxygenase, HEAT-like repeat-containing protein 1

7 Images
Immunocytochemistry/ Immunofluorescence - Anti-DOHH antibody (AB254866)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-DOHH antibody (AB254866)

PFA fixed, Triton X-100 permeabilized A-431 (Human epidermoid carcinoma cell line) cells labeling DOHH using ab254866 at 4µg/ml (green) in ICC/IF.

Western blot - Anti-DOHH antibody (AB254866)
  • WB

Supplier Data

Western blot - Anti-DOHH antibody (AB254866)

All lanes:

Western blot - Anti-DOHH antibody (ab254866) at 0.4 µg/mL

Lane 1:

Vector only transfected HEK-293T cell lysate

Lane 2:

DOHH overexpressed HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Predicted band size: 33 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOHH antibody (AB254866)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOHH antibody (AB254866)

Formalin-fixed, paraffin-embedded human kidney tissue stained for DOHH with ab254866 at a 1/200 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOHH antibody (AB254866)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOHH antibody (AB254866)

Formalin-fixed, paraffin-embedded human duodenum tissue stained for DOHH with ab254866 at a 1/200 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOHH antibody (AB254866)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOHH antibody (AB254866)

Formalin-fixed, paraffin-embedded human cerebellum tissue stained for DOHH with ab254866 at a 1/200 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOHH antibody (AB254866)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOHH antibody (AB254866)

Formalin-fixed, paraffin-embedded human hippocampus tissue stained for DOHH with ab254866 at a 1/200 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOHH antibody (AB254866)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOHH antibody (AB254866)

Formalin-fixed, paraffin-embedded human cerebral cortex tissue stained for DOHH with ab254866 at a 1/200 dilution in immunohistochemical analysis.

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

WB, IHC-P, ICC/IF

applications

免疫原

Recombinant Fragment Protein within Human DOHH aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

Q9BU89

style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200.00000 - 1/500.00000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04000-0.40000 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25000-2.00000 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100</p>" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column
style
left-column

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
存储溶液
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
style
left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The deoxyhypusine hydroxylase (DOHH) enzyme also known as hDOHH catalyzes the final step in the post-translational synthesis of the unusual amino acid hypusine. DOHH modifies deoxyhypusine into hypusine by hydroxylation a reaction critical in activating the eukaryotic initiation factor 5A (eIF5A). The human DOHH protein has a mass of approximately 36 kDa and is ubiquitously expressed in various tissues reflecting its general significance in protein processing.
Biological function summary

Proteins such as DOHH play important roles in cellular growth and proliferation. DOHH functions by converting inactive eIF5A precursors into their active form which can influence mRNA translation. This enzyme does not form part of a larger complex with other proteins but its activity is essential for the hypusine modification necessary to activate eIF5A directly influencing protein synthesis.

Pathways

DOHH operates in the hypusine biosynthesis pathway which is important for cell proliferation and differentiation. DOHH acts after the enzyme deoxyhypusine synthase (DHS) introduces a deoxyhypusine residue into eIF5A illustrating the close relationship between these two proteins in this cascade. The activation of eIF5A through hypusination impacts diverse pathways that regulate cell cycle progression and apoptosis.

DOHH activity has implications in cancer and neurodegenerative diseases. Altered expression or malfunction of DOHH can influence eIF5A activity affecting cell growth and survival and therefore contributing to oncogenesis. In neurodegenerative conditions abnormal hypusine synthesis may disrupt neuronal integrity and function. The linked protein eIF5A plays a role in these diseases demonstrating the broader impact of DOHH on health outcomes.
style
left-column
style
left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

靶点信息

Catalyzes the hydroxylation of the N(6)-(4-aminobutyl)-L-lysine intermediate produced by deoxyhypusine synthase/DHPS on a critical lysine of the eukaryotic translation initiation factor 5A/eIF-5A. This is the second step of the post-translational modification of that lysine into an unusual amino acid residue named hypusine (PubMed : 16371467, PubMed : 16533814, PubMed : 19706422). Hypusination is unique to mature eIF-5A factor and is essential for its function (By similarity).
See full target information DOHH
style
left-column
style
left-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。
style
left-column
style
right-column

关联产品

Select an associated product type
Alternative Product
Primary Antibodies

AB240975

Anti-DOHH antibody [EPR16730] - BSA and Azide free

primary-antibodies

dohh-antibody-epr16730-bsa-and-azide-free-ab240975

0

(0 reviews)

Alternative Product
Primary Antibodies

AB197587

Anti-DOHH antibody [EPR16730]

primary-antibodies

dohh-antibody-epr16730-ab197587

0

(0 reviews)

style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com