AB118790

Anti-DOCK7抗体

Name: DOCK7抗体 (ab118790)| Abcam中文官网
Brand: Abcam Limited
SKU: AB118790
Price: 4826 CNY
Availability: InStock

Anti-DOCK7 antibody

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(2 Publications)

Rabbit Polyclonal DOCK7 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human DOCK7 aa 150-250.

查看别名

KIAA1771, DOCK7, Dedicator of cytokinesis protein 7

2 Images

Unknown

Immunoprecipitation - Anti-DOCK7 antibody (AB118790)

Detection of DOCK7 in Immunoprecipitates of HeLa whole cell lysatse (1 mg for IP, 20% of IP loaded) using ab118790 at 6 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-DOCK7 antibody (ab118790)

Predicted band size: 243 kDa

false

Unknown

Western blot - Anti-DOCK7 antibody (AB118790)

All lanes:

Western blot - Anti-DOCK7 antibody (ab118790) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

293T whole cell lysate at 50 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Predicted band size: 243 kDa

true

Exposure time: 30s

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, IP

applications

免疫原

Synthetic Peptide within Human DOCK7 aa 150-250. The exact immunogen used to generate this antibody is proprietary information.

Q96N67

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Platypus": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

ab118790 was affinity purified using an epitope specific to DOCK7 immobilized on solid support.

存储溶液

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

DOCK7 a member of the Dedicator of Cytokinesis (DOCK) family is a guanine nucleotide exchange factor (GEF) that specifically activates Rac1 and Rac3 GTPases. The DOCK7 protein has a molecular mass of approximately 240 kDa. It is widely expressed in various tissues with significant levels found in the brain contributing to its critical functions in neuronal development.

Biological function summary

The protein plays an important role in neurogenesis and neuronal polarization. DOCK7 is not part of a complex itself but it interacts with other proteins to execute its functions. This protein influences neuronal morphology and axon formation by regulating cytoskeletal dynamics. Through these actions DOCK7 affects the movement and differentiation of neurons during development.

Pathways

The protein participates significantly in the Rac signaling pathway which is important for actin cytoskeleton reorganization. This involvement consequently impacts cellular processes like migration and cell shape regulation. DOCK7 directly relates to the functions of the Rac1 and Rac3 proteins. These proteins partake in pathways that guide axon guidance and neuronal migration emphasizing DOCK7’s role in neurodevelopment.

Dysfunctions involving the protein have associations with neurological conditions like epilepsy and learning disabilities. Mutations or dysregulation in DOCK7 can impact the function of Rac1 disrupting normal neuronal function and leading to disease phenotypes. Understanding these connections may offer insights into therapeutic targets for neurological disorders where these pathways are disrupted.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Functions as a guanine nucleotide exchange factor (GEF), which activates Rac1 and Rac3 Rho small GTPases by exchanging bound GDP for free GTP. Does not have a GEF activity for CDC42. Required for STMN1 'Ser-15' phosphorylation during axon formation and consequently for neuronal polarization (PubMed : 16982419). As part of the DISP complex, may regulate the association of septins with actin and thereby regulate the actin cytoskeleton (PubMed : 29467281). Has a role in pigmentation (By similarity). Involved in the regulation of cortical neurogenesis through the control of radial glial cells (RGCs) proliferation versus differentiation; negatively regulates the basal-to-apical interkinetic nuclear migration of RGCs by antagonizing the microtubule growth-promoting function of TACC3 (By similarity).

See full target information DOCK7

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Cell metabolism 33:2367-2379.e4 PubMed34767745

2021

Nutritional reprogramming of mouse liver proteome is dampened by metformin, resveratrol, and rapamycin.

Applications

Unspecified application

Species

Unspecified reactive species

David G Le Couteur,Samantha M Solon-Biet,Benjamin L Parker,Tamara Pulpitel,Amanda E Brandon,Nicholas J Hunt,Jibran A Wali,Rahul Gokarn,Alistair M Senior,Gregory J Cooney,David Raubenheimer,Victoria C Cogger,David E James,Stephen J Simpson

The Journal of cell biology 216:4313-4330 PubMed29089377

2017

Dual role for DOCK7 in tangential migration of interneuron precursors in the postnatal forebrain.

Applications

Unspecified application

Species

Unspecified reactive species

Shinichi Nakamuta,Yu-Ting Yang,Chia-Lin Wang,Nicholas B Gallo,Jia-Ray Yu,Yilin Tai,Linda Van Aelst

View all publications

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Anti-DOCK7抗体

Anti-DOCK7 antibody

Immunoprecipitation - Anti-DOCK7 antibody (AB118790)

Western blot - Anti-DOCK7 antibody (AB118790)