Anti-Dnmt3L抗体(ab3493)
Key features and details
- Rabbit polyclonal to Dnmt3L
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Dnmt3L抗体
参阅全部 Dnmt3L 一抗 -
描述
兔多克隆抗体to Dnmt3L -
宿主
Rabbit -
特异性
This antibody is expected to cross-react with mouse as the sequence of the human immunogen shows 76% identity with mouse. -
经测试应用
适用于: IPmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human Dnmt3L aa 152-164 conjugated to keyhole limpet haemocyanin.
Sequence:C-GLLQRRRKWRSQL
Database link: Q9UJW3 -
常规说明
DNMT 3L is a nuclear protein which has similarity to DNA methyltransferases, involved in de novo methylation of CpG islands. CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases. This protein is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, this protein does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and it is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1.
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性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. This antibody is expected to cross-react with mouse as the sequence of the human immunogen shows 76% identity with mouse. Reactivity with DNMT3L from other species has not yet been tested. -
Primary antibody说明
DNMT 3L is a nuclear protein which has similarity to DNA methyltransferases, involved in de novo methylation of CpG islands. CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases. This protein is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, this protein does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and it is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
应用 | Ab评论 | 说明 |
---|---|---|
IP | (6) |
1/100.
|
说明 |
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IP
1/100. |
靶标
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功能
Catalytically inactive regulatory factor of DNA methyltransferases. It is essential for the function of DNMT3A and DNMT3B. Activates DNMT3A and DNMT3B by binding to their catalytic domain. Accelerates the binding of DNA and AdoMet to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases. Recognizes unmethylated histone H3 lysine 4 (H3K4) and induces de novo DNA methylation by recruitment or activation of DNMT3. -
组织特异性
Expressed at low levels in several tissues including testis, ovary, and thymus. -
序列相似性
Belongs to the C5-methyltransferase family.
Contains 1 ADD-type zinc finger. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 29947 Human
- Omim: 606588 Human
- SwissProt: Q9UJW3 Human
- Unigene: 592165 Human
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别名
- Cytosine 5 methyltransferase 3 like protein antibody
- DNA (cytosine 5 ) methyltransferase 3 like antibody
- DNA (cytosine-5)-methyltransferase 3-like antibody
see all
图片
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Transfection of U2OS cells (10cm dish) using 3
µ g of GAL4-DNMT3L. Protein extraction using IPH 150mM. IP and WB conditions, see Fuks et al. (2000). Nature Genetics 24: 88-91.WB using 0.4µ g/ml in TBS milk 2%, BSA 0.5%. Dectection using ECL.Note: ab3493 worked in WB following IP, but did not work in a straight WB.
数据表及文件
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SDS download
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Datasheet download
文献 (14)
ab3493 被引用在 14 文献中.
- Wang Y et al. Retinoic acid induces NELFA-mediated 2C-like state of mouse embryonic stem cells associates with epigenetic modifications and metabolic processes in chemically defined media. Cell Prolif 54:e13049 (2021). PubMed: 33960560
- Wu B et al. Activin A and BMP4 Signaling Expands Potency of Mouse Embryonic Stem Cells in Serum-Free Media. Stem Cell Reports 14:241-255 (2020). PubMed: 32032551
- Ishihara T et al. DNA methylation dynamics in the germline of the marsupial tammar wallaby, Macropus eugenii. DNA Res 26:85-94 (2019). PubMed: 30535324
- Peng J et al. The detrimental effects of glucocorticoids exposure during pregnancy on offspring's cardiac functions mediated by hypermethylation of bone morphogenetic protein-4. Cell Death Dis 9:834 (2018). PubMed: 30082698
- Nanan KK et al. Independence between pre-mRNA splicing and DNA methylation in an isogenic minigene resource. Nucleic Acids Res 45:12780-12797 (2017). PubMed: 29244186