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Epigenetics and Nuclear Signaling DNA methylation DNMTs

Anti-Dnmt3a抗体(ab2850)

  • Datasheet
  • SDS
Reviews (11) Submit a question References (99)

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Western blot - Anti-Dnmt3a antibody (ab2850)
  • Western blot - Anti-Dnmt3a antibody (ab2850)
  • Western blot - Anti-Dnmt3a antibody (ab2850)

Key features and details

  • Rabbit polyclonal to Dnmt3a
  • Suitable for: WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-Dnmt3a antibody [EPR18455-94] (ab227823)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-Dnmt3a抗体
    参阅全部 Dnmt3a 一抗
  • 描述

    兔多克隆抗体to Dnmt3a
  • 宿主

    Rabbit
  • 特异性

    This antibody detects DNA methyltransferase 3a (Dnmt3a) from human and mouse tissues and cells as well as recombinant human Dnmt3a. This antibody does not detect full length recombinant human Dnmt3b or Dnmt1.
  • 经测试应用

    适用于: WBmore details
  • 种属反应性

    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Human Dnmt3a aa 50-150.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Epigenetics and Nuclear Signaling
    • DNA methylation
    • DNMTs

相关产品

  • ChIP Related Products

    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human Dnmt3a protein (ab170408)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab2850于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (4)
Use a concentration of 2 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 101.5 kDa).
说明
WB
Use a concentration of 2 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 101.5 kDa).

靶标

  • 功能

    Required for genome wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZNF238. Can actively repress transcription through the recruitment of HDAC activity.
  • 组织特异性

    Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung.
  • 序列相似性

    Belongs to the C5-methyltransferase family.
    Contains 1 ADD domain.
    Contains 1 GATA-type zinc finger.
    Contains 1 PHD-type zinc finger.
    Contains 1 PWWP domain.
  • 结构域

    The PWWP domain is essential for targeting to pericentric heterochromatin.
  • 翻译后修饰

    Sumoylated; sumoylation disrupts the ability to interact with histone deacetylases (HDAC1 and HDAC2) and repress transcription.
  • 细胞定位

    Nucleus. Cytoplasm. Accumulates in the major satellite repeats at pericentric heterochromatin.
  • Target information above from: UniProt accession Q9Y6K1 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 1788 Human
    • Entrez Gene: 13435 Mouse
    • Omim: 602769 Human
    • SwissProt: Q9Y6K1 Human
    • SwissProt: O88508 Mouse
    • Unigene: 515840 Human
    • Unigene: 5001 Mouse
    • 别名

      • DNA (cytosine 5) methyltransferase 3 alpha antibody
      • DNA (cytosine 5) methyltransferase 3A antibody
      • DNA (cytosine-5)-methyltransferase 3A antibody
      • DNA cytosine methyltransferase 3A2 antibody
      • DNA methyltransferase 3 alpha antibody
      • DNA methyltransferase 3a antibody
      • DNA methyltransferase HsaIIIA antibody
      • DNA MTase HsaIIIA antibody
      • DNM3A_HUMAN antibody
      • DNMT 3a antibody
      • DNMT antibody
      • Dnmt3a antibody
      • DNMT3A2 antibody
      • M.HsaIIIA antibody
      • MCMT antibody
      • OTTHUMP00000201149 antibody
      • TBRS antibody
      see all

    图片

    • Western blot - Anti-Dnmt3a antibody (ab2850)
      Western blot - Anti-Dnmt3a antibody (ab2850)Image from Nucleic Acids Res. 2014 Jan; 42(2): 804–821. Fig 5D, doi: 10.1093/nar/gkt920 Reproduced under the creative commons licence. https://creativecommons.org/licenses/by/4.0/

      Western blot analysis of DNMT3a in HeLa silenced cells. Total cell extracts were prepared 48 h after electroporation and analyzed by immunoblot with ab2850.

    • Western blot - Anti-Dnmt3a antibody (ab2850)
      Western blot - Anti-Dnmt3a antibody (ab2850)
      Western blot of Dnmt3a on P19 cell nuclear extract using ab2850.
    • Western blot - Anti-Dnmt3a antibody (ab2850)
      Western blot - Anti-Dnmt3a antibody (ab2850)
      All lanes : Anti-Dnmt3a antibody (ab2850) at 1/500 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : HEK293 cell lysate
      Lane 4 : A549 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit IgG (H+L), Superclonal™ HRP at 1/1000 dilution

      Predicted band size: 101.5 kDa
      Observed band size: 120 kDa why is the actual band size different from the predicted?

    实验方案

    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (99)

    发表研究结果有使用 ab2850?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab2850 被引用在 99 文献中.

    • Palomares KT  et al. Fetal Exosomal Platelet-activating Factor Triggers Functional Progesterone Withdrawal in Human Placenta. Reprod Sci 28:252-262 (2021). PubMed: 32780361
    • Wang Y  et al. LINC00922 regulates epithelial-mesenchymal transition, invasive and migratory capacities in breast cancer through promoting NKD2 methylation. Cell Signal 77:109808 (2021). PubMed: 33045317
    • González-Rodríguez P  et al. The DNA methyltransferase DNMT3A contributes to autophagy long-term memory. Autophagy 17:1259-1277 (2021). PubMed: 32876528
    • Xu L  et al. Abnormal neocortex arealization and Sotos-like syndrome-associated behavior in Setd2 mutant mice. Sci Adv 7:N/A (2021). PubMed: 33523829
    • Shao G  et al. Methylation-dependent MCM6 repression induced by LINC00472 inhibits triple-negative breast cancer metastasis by disturbing the MEK/ERK signaling pathway. Aging (Albany NY) 13:4962-4975 (2021). PubMed: 33668040
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
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    1-10 of 11 Abreviews or Q&A

    Western blot abreview for Anti-Dnmt3a antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Guinea pig Cell lysate - whole cell (Liver)
    Gel Running Conditions
    Reduced Denaturing (Concentrating 4%, Separating 6%)
    Loading amount
    50 µg
    Specification
    Liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Oct 25 2019

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Dnmt3a antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Spinal cord)
    Antigen retrieval step
    None
    Permeabilization
    Yes - 0.2% Triton
    Specification
    Spinal cord
    Blocking step
    5% Donkey Serum, 3% BSA as blocking agent for 30 minute(s) · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Miss. Menglu Qian

    Verified customer

    提交于 Aug 01 2019

    Western blot abreview for Anti-Dnmt3a antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (HEK and NRK)
    Gel Running Conditions
    Reduced Denaturing (6)
    Loading amount
    20 µg
    Specification
    HEK and NRK
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Apr 28 2017

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Dnmt3a antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (dorsal root ganglion and spinal cord (P4))
    Permeabilization
    Yes - 0.2% Triton
    Specification
    dorsal root ganglion and spinal cord (P4)
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1.5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Franziska Denk

    Verified customer

    提交于 Mar 22 2017

    ChIP abreview for Anti-Dnmt3a antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    ChIP
    Sample
    Human Cell lysate - whole cell (Breast cancer line)
    Specification
    Breast cancer line
    Detection step
    Real-time PCR
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 10 minute(s) and 0 second(s)
    Specification of the cross-linking agent: Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Mar 14 2017

    Western blot abreview for Anti-Dnmt3a antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Breast cancer line)
    Gel Running Conditions
    Reduced Denaturing (12.5%)
    Loading amount
    100000 cells
    Specification
    Breast cancer line
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Mar 07 2017

    Western blot abreview for Anti-Dnmt3a antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (Gonad)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    75 µg
    Specification
    Gonad
    Blocking step
    Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Nov 11 2015

    ChIP abreview for Anti-Dnmt3a antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    ChIP
    Sample
    Human Cell lysate - other (Epithelial cells)
    Negative control
    IgG
    Specification
    Epithelial cells
    Detection step
    Real-time PCR
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 10 minute(s) and 0 second(s)
    Positive control
    Input DNA
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Sep 30 2015

    ChIP abreview for Anti-Dnmt3a antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    ChIP
    Detection step
    Real-time PCR
    Sample
    Rat Tissue lysate - whole (Gonad)
    Specification
    Gonad
    Negative control
    IgG
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 0 second(s)
    Positive control
    Input DNA
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jul 07 2014

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Dnmt3a antibody - ChIP Grade

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
    Sample
    Marmoset (common) Tissue sections (Adult testis)
    Specification
    Adult testis
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. 煜欽 林

    Verified customer

    提交于 Jan 21 2014

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