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重组RabMAb

重组Anti-DNase I抗体[EPR25941-20] - BSA and Azide free (ab317684)

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Western blot - Anti-DNase I antibody [EPR25941-20] - BSA and Azide free (ab317684)
  • Western blot - Anti-DNase I antibody [EPR25941-20] - BSA and Azide free (ab317684)
  • Anti-DNase I antibody [EPR25941-20] - BSA and Azide free (ab317684)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR25941-20] to DNase I - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-DNase I抗体[EPR25941-20] - BSA and Azide free
    参阅全部 DNase I 一抗

  • 描述

    兔单克隆抗体[EPR25941-20] to DNase I - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WBmore details
    不适用于: Flow Cyt (Intra),ICC/IF,IHC-P or IP

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: HeLa whole cell, HeLa transfected with siRNA specifically targeting DNase I whole cell, SH-SY5Y whole cell, NIH/3T3 whole cell, PC-12 whole cell, Human stomach tissue and Human pancreas tissue lysates.

  • 常规说明

    ab317684 is the carrier-free version of ab317683

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab317684于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use at an assay dependent concentration. Predicted molecular weight: 31 kDa.
说明
WB
Use at an assay dependent concentration. Predicted molecular weight: 31 kDa.
应用说明
Is unsuitable for Flow Cyt (Intra),ICC/IF,IHC-P or IP.

靶标

  • 功能

    Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization.

  • 组织特异性

    Principally in tissues of the digestive system. Highest levels found in urine, but also relatively abundant in semen and saliva.

  • 序列相似性

    Belongs to the DNase I family.

  • 细胞定位

    Secreted. Nucleus envelope. Secretory protein, stored in zymogen granules and found in the nuclear envelope.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • Deoxyribonuclease 1 antibody
    • Deoxyribonuclease I antibody
    • Deoxyribonuclease-1 antibody
    • Deoxyribonuclease1 antibody
    • DeoxyribonucleaseI antibody
    • DNAS1_HUMAN antibody
    • DNASE 1 antibody
    • DNase I antibody
    • DNase I lysosomal antibody
    • DNASE1 antibody
    • DNaseI antibody
    • DNL 1 antibody
    • DNL1 antibody
    • Dornase alfa antibody
    • DRNI antibody
    • FLJ38093 antibody
    • Human urine deoxyribonuclease I antibody
    see all

图片

  • Western blot - Anti-DNase I antibody [EPR25941-20] - BSA and Azide free (ab317684)
    Western blot - Anti-DNase I antibody [EPR25941-20] - BSA and Azide free (ab317684)
    All lanes : Anti-DNase I antibody [EPR25941-20] (ab317683) at 1/1000 dilution

    Lane 1 : HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate
    Lane 2 : HeLa transfected with siRNA specifically targeting DNase I whole cell lysate
    Lane 3 : SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate
    Lane 4 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
    Lane 5 : PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 31 kDa
    Observed band size: 36 kDa why is the actual band size different from the predicted?



    This data was developed using ab317683, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The bands observed at higher mw could be detection of post-translational modifications (PMID: 34329318) or glycosylated forms of the protein (PMID:11256970).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control  (ab181602) staining at 1/200000 dilution.

    Exposure time: Lanes 1-3, 5: 10 seconds, lane 4: 26 seconds

  • Western blot - Anti-DNase I antibody [EPR25941-20] - BSA and Azide free (ab317684)
    Western blot - Anti-DNase I antibody [EPR25941-20] - BSA and Azide free (ab317684)
    All lanes : Anti-DNase I antibody [EPR25941-20] (ab317683) at 1/1000 dilution

    Lane 1 : Human stomach tissue lysate
    Lane 2 : Human pancreas tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 31 kDa
    Observed band size: 36 kDa why is the actual band size different from the predicted?



    This data was developed using ab317683, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The bands observed at higher mw could be detection of post-translational modifications (PMID: 34329318) or glycosylated forms of the protein (PMID:11256970).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control  (ab181602) staining at 1/200000 dilution.

    Exposure time: Lane 1: 48 seconds, lane 2: 15 seconds

  • Anti-DNase I antibody [EPR25941-20] - BSA and Azide free (ab317684)
    Anti-DNase I antibody [EPR25941-20] - BSA and Azide free (ab317684)

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (0)

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