Anti-DNAJB9抗体(ab118282)
Key features and details
- Goat polyclonal to DNAJB9
- Suitable for: WB, ICC, Flow Cyt (Intra)
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-DNAJB9抗体
参阅全部 DNAJB9 一抗 -
描述
山羊多克隆抗体to DNAJB9 -
宿主
Goat -
经测试应用
适用于: WB, ICC, Flow Cyt (Intra)more details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Cow, Dog, Pig, Chimpanzee
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免疫原
Synthetic peptide:
PDAEAKFREIAEAYE
with a Cysteine residue linker, corresponding to internal sequence amino acids 61-75 of Human DNAJB9 (NP_036460.1). -
阳性对照
- WB: Human liver and kidney lysates. Mouse liver lysate; ICC: A431 and U2OS cells; Flow Cyt (intra): A431 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab118282 is purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab118282于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
1 hour primary incubation is recommended for this product. |
|
| ICC |
Use a concentration of 10 µg/ml.
|
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| Flow Cyt (Intra) |
Use a concentration of 10 µg/ml.
|
| 说明 |
|---|
|
WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa). 1 hour primary incubation is recommended for this product. |
|
ICC
Use a concentration of 10 µg/ml. |
|
Flow Cyt (Intra)
Use a concentration of 10 µg/ml. |
靶标
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功能
Acts as a co-chaperone with an Hsp70 protein. -
序列相似性
Contains 1 J domain. -
细胞定位
Cytoplasm. Nucleus. Stress induces its translocation to the nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 614588 Cow
- Entrez Gene: 4189 Human
- Entrez Gene: 27362 Mouse
- Entrez Gene: 24908 Rat
- Omim: 602634 Human
- SwissProt: Q9UBS3 Human
- SwissProt: Q9QYI6 Mouse
- SwissProt: P97554 Rat
see all -
别名
- DKFZP564F1862 antibody
- DnaJ (Hsp40) homolog subfamily B member 9 antibody
- DnaJ homolog subfamily B member 9 antibody
see all
图片
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Western blot - Anti-DNAJB9 antibody (ab118282)All lanes : Anti-DNAJB9 antibody (ab118282) at 1 µg/ml
Lane 1 : Human liver lysate (in RIPA buffer)
Lane 2 : Human kidney lysate (in RIPA buffer)
Lane 3 : Mouse liver lysate (in RIPA buffer)
Lysates/proteins at 35 µg per lane.
Predicted band size: 26 kDaPrimary incubation 1 hour at room temperature. Detected by chemiluminescence.
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Immunocytochemistry - Anti-DNAJB9 antibody (ab118282)Immunocytochemistry analysis of paraformaldehyde fixed U2OS cells labeling DNAJB9 with ab118282. Cells permeabilized with 0.15% Triton. Primary incubation 1 hour (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (2 µg/mL). The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (2 µg/mL).
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Immunocytochemistry - Anti-DNAJB9 antibody (ab118282)Immunocytochemistry analysis of paraformaldehyde fixed A431 cells labeling DNAJB9 with ab118282. Cells permeabilized with 0.15% Triton. Primary incubation 1 hour (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (2 µg/mL). The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (2 µg/mL).
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Flow Cytometry (Intracellular) - Anti-DNAJB9 antibody (ab118282)Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line) labeling DNAJB9 with ab118282. Cells permeabilized with 0.5% Triton. Primary incubation 1 hour (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor® 488 secondary antibody.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab118282 被引用在 4 文献中.
- Bestepe F et al. Deficiency of miR-409-3p improves myocardial neovascularization and function through modulation of DNAJB9/p38 MAPK signaling. Mol Ther Nucleic Acids 32:995-1009 (2023). PubMed: 37332476
- Rashid F et al. The ORF8 protein of SARS-CoV-2 induced endoplasmic reticulum stress and mediated immune evasion by antagonizing production of interferon beta. Virus Res 296:198350 (2021). PubMed: 33626380
- Yoneda A et al. Heat Shock Protein 47 Maintains Cancer Cell Growth by Inhibiting the Unfolded Protein Response Transducer IRE1a. Mol Cancer Res 18:847-858 (2020). PubMed: 32102897
- Liu Z et al. Reducing Smad3/ATF4 was essential for Sirt1 inhibiting ER stress-induced apoptosis in mice brown adipose tissue. Oncotarget 8:9267-9279 (2017). WB . PubMed: 28030827