Anti-DNAJB8抗体(ab235546)
Key features and details
- Rabbit polyclonal to DNAJB8
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-DNAJB8抗体 -
描述
兔多克隆抗体to DNAJB8 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant full length protein corresponding to Human DNAJB8 aa 1 to the C-terminus.
Database link: Q8NHS0 -
阳性对照
- WB: HeLa whole cell lysate; Mouse brain and liver tissue lysates. IHC-P: Human placenta and rectum tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Protein G purified -
纯化说明
Purity >95% -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab235546于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/2000. Predicted molecular weight: 26 kDa.
|
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| IHC-P |
1/20 - 1/200.
|
| 说明 |
|---|
|
WB
1/500 - 1/2000. Predicted molecular weight: 26 kDa. |
|
IHC-P
1/20 - 1/200. |
靶标
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序列相似性
Contains 1 J domain. - Information by UniProt
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数据库链接
- Entrez Gene: 165721 Human
- Entrez Gene: 56691 Mouse
- Omim: 611337 Human
- SwissProt: Q8NHS0 Human
- SwissProt: Q9QYI7 Mouse
- Unigene: 518241 Human
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别名
- DJ6 antibody
- DnaJ (Hsp40) homolog, subfamily B, member 8 antibody
- DnaJ homolog subfamily B member 8 antibody
see all
图片
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Western blot - Anti-DNAJB8 antibody (ab235546)All lanes :
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : Mouse liver tissue lysate
Secondary
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 26 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJB8 antibody (ab235546)Paraffin-embedded human placenta tissue stained for DNAJB8 using ab235546 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJB8 antibody (ab235546)Paraffin-embedded human rectum tissue stained for DNAJB8 using ab235546 at 1/100 dilution in immunohistochemical analysis.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab235546 被引用在 2 文献中.
- Wang Z et al. DNAJB8 in small extracellular vesicles promotes Oxaliplatin resistance through TP53/MDR1 pathway in colon cancer. Cell Death Dis 13:151 (2022). PubMed: 35165262
- Ryder BD et al. Regulatory inter-domain interactions influence Hsp70 recruitment to the DnaJB8 chaperone. Nat Commun 12:946 (2021). PubMed: 33574241