AB250838

重组Anti-DNAJA4抗体[EPR8756] - BSA and Azide free

Anti-DNAJA4 antibody [EPR8756] - BSA and Azide free

RabMAb
Recombinant
了解详情

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Rabbit Recombinant Monoclonal DNAJA4 antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples.

查看别名

DnaJ homolog subfamily A member 4, DNAJA4

3 Images

Flow Cytometry (Intracellular) - Anti-DNAJA4 antibody [EPR8756] - BSA and Azide free (AB250838)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-DNAJA4 antibody [EPR8756] - BSA and Azide free (AB250838)

This data was developed using ab185553, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of DNAJA4 in 2% paraformaldehyde HeLa cells using ab185553 at 1/300 dilution (pink). Secondary antibody : Goat anti rabbit IgG (FITC) at 1/150 dilution. Isotype control : Rabbit monoclonal IgG (green).

Unknown

Immunoprecipitation - Anti-DNAJA4 antibody [EPR8756] - BSA and Azide free (AB250838)

This data was developed using ab185553, the same antibody clone in a different buffer formulation.

Western blot analysis of Immunoprecipitate from Human fetal brain lysate (lane 1) using ab185553 at 1/50 dilution. Lane 2 : PBS used instead of Human fetal brain lysates. Secondary antibody : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, at 1/1500 dilution. Blocking buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-DNAJA4 antibody [EPR8756] (<a href='/products/primary-antibodies/dnaja4-antibody-epr8756-ab185553'>ab185553</a>)

Predicted band size: 45 kDa

false

Supplier Data

Western blot - Anti-DNAJA4 antibody [EPR8756] - BSA and Azide free (AB250838)

This data was developed using ab185553, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-DNAJA4 antibody [EPR8756] (<a href='/products/primary-antibodies/dnaja4-antibody-epr8756-ab185553'>ab185553</a>) at 1/10000 dilution

Lane 1:

Human fetal brain tissue lysate at 20 µg

Lane 2:

Hela cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 45 kDa

false

不同偶联物与剂型 (1)

Unconjugated

Anti-DNAJA4 antibody [EPR8756]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR8756

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

IP, WB, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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产品详情

ab250838 is the carrier-free version of ab185553.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

See full target information DNAJA4

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-DNAJA4抗体[EPR8756] - BSA and Azide free