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AB185553

重组Anti-DNAJA4抗体[EPR8756]

Anti-DNAJA4 antibody [EPR8756]

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(2 Publications)

Rabbit Recombinant Monoclonal DNAJA4 antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

查看别名

DnaJ homolog subfamily A member 4, DNAJA4

3 Images
Flow Cytometry (Intracellular) - Anti-DNAJA4 antibody [EPR8756] (AB185553)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-DNAJA4 antibody [EPR8756] (AB185553)

Intracellular flow cytometric analysis of DNAJA4in 2% paraformaldehydeHeLa cellsusing ab185553 at 1/300 dilution (pink). Secondary antibody : Goat anti rabbit IgG (FITC) at 1/150 dilution. Isotype control : Rabbit monoclonal IgG (green).

Immunoprecipitation - Anti-DNAJA4 antibody [EPR8756] (AB185553)
  • IP

Unknown

Immunoprecipitation - Anti-DNAJA4 antibody [EPR8756] (AB185553)

Western blot analysis of Immunoprecipitate from Human fetal brain lysate (lane 1) using ab185553 at 1/50 dilution. Lane 2 : PBS used instead of Human fetal brain lysates. Secondary antibody : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, at 1/1500 dilution. Blocking buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-DNAJA4 antibody [EPR8756] (ab185553)

Predicted band size: 45 kDa

false

Western blot - Anti-DNAJA4 antibody [EPR8756] (AB185553)
  • WB

Supplier Data

Western blot - Anti-DNAJA4 antibody [EPR8756] (AB185553)

Blocking buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-DNAJA4 antibody [EPR8756] (ab185553) at 1/10000 dilution

Lane 1:

Human fetal brain tissue lysate at 20 µg

Lane 2:

Hela cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 45 kDa

false

不同偶联物与剂型 (1)

  • Carrier free

    Anti-DNAJA4 antibody [EPR8756] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR8756

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

IP, Flow Cyt (Intra), WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30 - 1/50", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/300", "FlowCytIntra-species-notes": "<p><a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

产品详情

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
运输条件
Conditional Ambient
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 14:697 PubMed37875476

2023

DNAJA4 suppresses epithelial-mesenchymal transition and metastasis in nasopharyngeal carcinoma via PSMD2-mediated MYH9 degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Qun Zhang,Ping Feng,Xun-Hua Zhu,Shi-Qing Zhou,Ming-Liang Ye,Xiao-Jing Yang,Sha Gong,Sheng-Yan Huang,Xi-Rong Tan,Shi-Wei He,Ying-Qing Li

Journal of dermatological science 91:256-267 PubMed29807809

2018

DNAJA4 deficiency enhances NF-kappa B-related growth arrest induced by hyperthermia in human keratinocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Zhe Sun,Yi Ren,Yu-Jing Zhang,Yang Han,Yang Yang,Ya-Li Gao,Li-Li Zhu,Rui-Qun Qi,Hong-Duo Chen,Xing-Hua Gao
View all publications

Abcam Product Promise

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