Anti-DNA2抗体(ab96488)
Key features and details
- Rabbit polyclonal to DNA2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-DNA2抗体
参阅全部 DNA2 一抗 -
描述
兔多克隆抗体to DNA2 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide corresponding to Human DNA2 aa 1000 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab42548) -
阳性对照
- This antibody gave a positive signal in the following Human Whole Cell lysates; HEK293, HEK293 (Nuclear) and U2OS and FFPE human liver tissue sections.
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常规说明
ab96488 is raised using the same immunogen as ab42439. Batches sold under ab96488 have passed QC testing in Western Blot. In our hands, this product did not work in ICC/IF. For further information please contact our Customer Services team.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab96488于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 125 kDa (predicted molecular weight: 120 kDa).
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| IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| 说明 |
|---|
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 125 kDa (predicted molecular weight: 120 kDa). |
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IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
May function in chromosomal DNA replication. -
序列相似性
Belongs to the DNA2/NAM7 helicase family. - Information by UniProt
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数据库链接
- Entrez Gene: 1763 Human
- Entrez Gene: 327762 Mouse
- Entrez Gene: 309762 Rat
- Omim: 601810 Human
- SwissProt: P51530 Human
- SwissProt: Q6ZQJ5 Mouse
- SwissProt: D3ZG52 Rat
- Unigene: 532446 Human
see all -
别名
- DNA replication ATP-dependent helicase-like homolog antibody
- DNA replication helicase 2 homolog antibody
- DNA replication helicase 2, yeast, homolog of antibody
see all
图片
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Western blot - Anti-DNA2 antibody (ab96488)All lanes : Anti-DNA2 antibody (ab96488) at 1 µg/ml
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Nuclear Lysate
Lane 3 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 120 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?
Additional bands at: 16 kDa, 18 kDa, 210 kDa, 37 kDa, 38 kDa, 54 kDa, 60 kDa, 70 kDa, 76 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 minutes
This antibody detects multiple bands in addition to the band of interest, reflecting low levels of expression of DNA2. siRNA knockdown experiments have demonstrated that this antibody detects a band at ~120 kDa corresponding to human DNA2 (please see image courtesy of Julien Duxin). -
Western blot - Anti-DNA2 antibody (ab96488)Image courtesy of Julien Duxin and Sheila StewartAll lanes : Anti-DNA2 antibody (ab96488)
Lane 1 : U2OS cells plus control shRNA
Lane 2 : U2OS cells plus shDNA2-1 shRNA
Lane 3 : U2OS cells plus shDNA2-2 shRNA
Secondary
All lanes : Anti-rabbit at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 120 kDa
Observed band size: 120 kDa
Western blot showing detection of DNA2 by ab42439. A 120 kDa band corresponding to DNA2 was detected in lysates of U2OS cells treated with control shRNA (lane 1) but not in those from knockdown cells treated with two different shRNAs for DNA2 (lanes 2 and 3). Following pre-blocking for 30 mins in 5% milk, PBS-Tween, the membrane was incubated overnight at 4°C with ab42439 at a dilution of 1/1000 in 5% milk, PBS-Tween. The membrane was then incubated with the secondary antibody at 1/3000 dilution in 5% milk, PBS-Tween for 2 hours at RT, and washed 3 x 10 mins with PBS-Tween prior to ECL detection. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA2 antibody (ab96488)IHC image of ab96488 staining in human liver carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab96488, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
数据表及文件
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SDS download
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Datasheet download
文献 (28)
ab96488 被引用在 28 文献中.
- Zhang J et al. Flap endonuclease 1 and DNA-PKcs synergistically participate in stabilizing replication fork to encounter replication stress in glioma cells. J Exp Clin Cancer Res 41:140 (2022). PubMed: 35414100
- Zhang H et al. TDP1-independent pathways in the process and repair of TOP1-induced DNA damage. Nat Commun 13:4240 (2022). PubMed: 35869071
- Wu X & Wang B Abraxas suppresses DNA end resection and limits break-induced replication by controlling SLX4/MUS81 chromatin loading in response to TOP1 inhibitor-induced DNA damage. Nat Commun 12:4373 (2021). PubMed: 34272385
- Datta A et al. WRN helicase safeguards deprotected replication forks in BRCA2-mutated cancer cells. Nat Commun 12:6561 (2021). PubMed: 34772932
- Kim JJ et al. PCAF-Mediated Histone Acetylation Promotes Replication Fork Degradation by MRE11 and EXO1 in BRCA-Deficient Cells. Mol Cell 80:327-344.e8 (2020). PubMed: 32966758