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Anti-DNA Polymerase theta抗体(ab111218)

Reviews (2) Submit a question References (1)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA Polymerase theta antibody (ab111218)

    Key features and details

    • Rabbit polyclonal to DNA Polymerase theta
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

    概述

    • 产品名称

      Anti-DNA Polymerase theta抗体

    • 描述

      兔多克隆抗体to DNA Polymerase theta

    • 宿主

      Rabbit

    • 经测试应用

      适用于: IHC-Pmore details

    • 种属反应性

      与反应: Human

    • 免疫原

      Synthetic peptide: H FSSLDIAV CT IERANGL INR LIEENK MDLL GMVVV DELHM LGDS HRGYLL, corresponding to amino acids 180-230 of Human DNA Polymerase theta

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • 阳性对照

      • Human breast carcinoma tissue.

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab111218于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IHC-P
    1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    说明
    IHC-P
    1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    靶标

    • 功能

      Has a DNA polymerase activity on nicked double-stranded DNA and on a singly primed DNA template. The enzyme activity is resistant to aphidicolin, and inhibited by dideoxynucleotides. Exhibites a single-stranded DNA-dependent ATPase activity. Could be involved in the repair of interstrand cross-links.

    • 组织特异性

      Highly expressed in testis.

    • 序列相似性

      Belongs to the DNA polymerase type-A family.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
    • Information by UniProt

    • 数据库链接

      • 别名

        • DKFZp781A0112 antibody
        • DNA polymerase eta antibody
        • DNA polymerase theta antibody
        • DPOLQ_HUMAN antibody
        • POLQ antibody
        • Polymerase (DNA directed) theta antibody
        • PRO0327 antibody
        see all

      图片

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA Polymerase theta antibody (ab111218)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA Polymerase theta antibody (ab111218)
        ab111218 at 1/50 dilution staining DNA Polymerase theta in paraffin-embedded Human Breast carcinoma tissue by Immunohistochemistry. The image on the right is treated with the synthesized peptide.

      文献 (1)

      发表研究结果有使用 ab111218？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab111218 被引用在 1 文献中.

      • Smolinska A  et al. DNA Polymerase Theta Plays a Critical Role in Pancreatic Cancer Development and Metastasis. Cancers (Basel) 14:N/A (2022). PubMed: 36077614

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      1-2 of 2 Abreviews or Q&A

      Immunocytochemistry/ Immunofluorescence abreview for Anti-DNA Polymerase theta antibody

       Inconclusive
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (Cardiomyocyte)
      Permeabilization
      Yes - 0.25% Triton X-100
      Specification
      Cardiomyocyte
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
      Fixative
      Paraformaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Jan 21 2022

      Western blot abreview for Anti-DNA Polymerase theta antibody

       Inconclusive
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (HeLa, U2OS, HEK293FT cell lines)
      Gel Running Conditions
      Reduced Denaturing (10% SDS-PAGE)
      Loading amount
      40 µg
      Specification
      HeLa, U2OS, HEK293FT cell lines
      Blocking step
      Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 4°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Oct 07 2020

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