Anti-DNA Polymerase Kappa/POLK抗体(ab115625)
Key features and details
- Goat polyclonal to DNA Polymerase Kappa/POLK
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-DNA Polymerase Kappa/POLK抗体
参阅全部 DNA Polymerase Kappa/POLK 一抗 -
描述
山羊多克隆抗体to DNA Polymerase Kappa/POLK -
宿主
Goat -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse
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免疫原
Synthetic peptide:
PVNQPKESSRSTGS
corresponding to amino acids 817-830 of Human DNA Polymerase Kappa/POLK, conjugated to KLH. -
阳性对照
- Human prostate tissue
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab115625于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
|
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. Lacks 3'-5' proofreading exonuclease activity. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity. -
组织特异性
Detected at low levels in testis, spleen, prostate and ovary. Detected at very low levels in kidney, colon, brain, heart, liver, lung, placenta, pancreas and peripheral blood leukocytes. -
序列相似性
Belongs to the DNA polymerase type-Y family.
Contains 2 UBZ-type zinc fingers.
Contains 1 umuC domain. -
结构域
The catalytic core consists of fingers, palm and thumb subdomains, but the fingers and thumb subdomains are much smaller than in high-fidelity polymerases; residues from five sequence motifs of the Y-family cluster around an active site cleft that can accommodate DNA and nucleotide substrates with relaxed geometric constraints, with consequently higher rates of misincorporation and low processivity. -
细胞定位
Nucleus. Detected throughout the nucleus and at replication foci. - Information by UniProt
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数据库链接
- Entrez Gene: 51426 Human
- Entrez Gene: 27015 Mouse
- Omim: 605650 Human
- SwissProt: Q9UBT6 Human
- SwissProt: Q9QUG2 Mouse
- Unigene: 726312 Human
- Unigene: 89926 Mouse
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别名
- DinB homolog 1 (E. coli) antibody
- DINB protein antibody
- DINB1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA Polymerase Kappa/POLK antibody (ab115625)ab115625, at 5 µg/ml, staining DNA Polymerase Kappa/POLK in formalin fixed, paraffin embedded Human prostate tissue by Immunohistochemistry. Following primary incubation slides were incubated with biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab115625 尚未被引用在任何文献中。