AB236450

Anti-DNA polymerase eta 抗体

Name: DNA polymerase eta抗体 (ab236450)| Abcam中文官网
Brand: Abcam Limited
SKU: AB236450
Price: 4864 CNY
Availability: InStock

Anti-DNA polymerase eta antibody

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(3 Publications)

Rabbit Polyclonal DNA polymerase eta antibody. Suitable for IP, Flow Cyt, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human POLH.

查看别名

RAD30, RAD30A, XPV, POLH, DNA polymerase eta, RAD30 homolog A, Xeroderma pigmentosum variant type protein

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-DNA polymerase eta antibody (AB236450)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DNA polymerase eta antibody (AB236450)

U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for DNA polymerase eta (green) using ab236450 at 1/2000 dilution in ICC/IF (green). The nuclear counter stain is DAPI (blue). The right-hand panel shows the merged images.

Flow Cytometry - Anti-DNA polymerase eta antibody (AB236450)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-DNA polymerase eta antibody (AB236450)

Flow cytometric analysis of 1% PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cell line labeling DNA polymerase eta with ab236450 at 1/200 dilution (green) compared with a no primary antibody control (blue).

Supplier Data

Immunoprecipitation - Anti-DNA polymerase eta antibody (AB236450)

DNA polymerase eta was immunoprecipitated from ~400 μg U-2 OS (human bone osteosarcoma epithelial cell line) whole cell extract with 3 μl ab236450. Western blot was performed from the immunoprecipitate using ab236450 at 1/2000 dilution.

Lane 1 : U-2 OS whole cell extract (Input)

Lane 2 : Control IgG IP in U-2 OS whole cell extract.

Lane 3 : ab236450 IP in U-2 OS whole cell extract.

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Immunoprecipitation - Anti-DNA polymerase eta antibody (ab236450)

Predicted band size: 78 kDa

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Supplier Data

Western blot - Anti-DNA polymerase eta antibody (AB236450)

Blocking buffer : 5% milk in TBS-T

Incubated overnight with primary antibody.

All lanes:

Western blot - Anti-DNA polymerase eta antibody (ab236450) at 1/2000 dilution

Lane 1:

U-2 OS (human bone osteosarcoma epithelial cell line) treated with control siRNA, whole cell lysate at 40 µg

Lane 2:

U-2 OS treated with DNA polymerase eta siRNA, whole cell lysate at 40 µg

Lane 3:

MEF (mouse embryonic fibroblast cell line) whole cell lysate at 40 µg

Lane 4:

INS-1 (rat pancreatic beta cell line) whole cell lysate at 40 µg

Predicted band size: 78 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

IP, Flow Cyt, WB, ICC/IF

applications

免疫原

Recombinant Fragment Protein within Human POLH. The exact immunogen used to generate this antibody is proprietary information.

Q9Y253

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "Use 3 μl to ~400 μg of extact.", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/2000", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯度

Whole antiserum

存储溶液

Preservative: 0.05% Sodium azide

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

DNA polymerase specifically involved in the DNA repair by translesion synthesis (TLS) (PubMed : 10385124, PubMed : 11743006, PubMed : 16357261, PubMed : 20388628, PubMed : 24449906, PubMed : 24553286, PubMed : 38212351). Due to low processivity on both damaged and normal DNA, cooperates with the heterotetrameric (REV3L, REV7, POLD2 and POLD3) POLZ complex for complete bypass of DNA lesions. Inserts one or 2 nucleotide(s) opposite the lesion, the primer is further extended by the tetrameric POLZ complex. In the case of 1,2-intrastrand d(GpG)-cisplatin cross-link, inserts dCTP opposite the 3' guanine (PubMed : 24449906). Particularly important for the repair of UV-induced pyrimidine dimers (PubMed : 10385124, PubMed : 11743006). Although inserts the correct base, may cause base transitions and transversions depending upon the context. May play a role in hypermutation at immunoglobulin genes (PubMed : 11376341, PubMed : 14734526). Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have any lyase activity, preventing the release of the 5'-deoxyribose phosphate (5'-dRP) residue. This covalent trapping of the enzyme by the 5'-dRP residue inhibits its DNA synthetic activity during base excision repair, thereby avoiding high incidence of mutagenesis (PubMed : 14630940). Targets POLI to replication foci (PubMed : 12606586).

See full target information POLH

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

British journal of pharmacology 182:3594-3611 PubMed40194519

2025

Inhibition of DNA polymerase eta-mediated translesion DNA synthesis with small molecule sensitises ovarian cancer stem-like cells to chemotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Subhankar Bose,Priyanka Saha,Md Tanjim Alam,Bilash Chatterjee,Mrinmoy Sarkar,Amit Kumar Dixit,Deepak Kumar,Rakesh Kumar Pathak,Prem Prakash Tripathi,Amit Kumar Srivastava

Genomics, proteomics & bioinformatics 20:597-613 PubMed33607295

2021

Phosphoproteomics Reveals the AMPK Substrate Network in Response to DNA Damage and Histone Acetylation.

Applications

Unspecified application

Species

Unspecified reactive species

Yuejing Jiang,Xiaoji Cong,Shangwen Jiang,Ying Dong,Lei Zhao,Yi Zang,Minjia Tan,Jia Li

Cell reports 31:107542 PubMed32375027

2020

Systematic Analysis of Targets of Pumilio-Mediated mRNA Decay Reveals that PUM1 Repression by DNA Damage Activates Translesion Synthesis.

Applications

WB, IF

Species

Unspecified reactive species, Unspecified reactive species

Toshimichi Yamada,Naoto Imamachi,Katsutoshi Imamura,Kenzui Taniue,Takeshi Kawamura,Yutaka Suzuki,Masami Nagahama,Nobuyoshi Akimitsu

View all publications

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Abcam Product Promise

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Anti-DNA polymerase eta 抗体

Anti-DNA polymerase eta antibody

Immunocytochemistry/ Immunofluorescence - Anti-DNA polymerase eta antibody (AB236450)

Flow Cytometry - Anti-DNA polymerase eta antibody (AB236450)

Immunoprecipitation - Anti-DNA polymerase eta antibody (AB236450)

Western blot - Anti-DNA polymerase eta antibody (AB236450)

关键信息

宿主种属

克隆

亚型

不含载体蛋白

反应种属

应用

免疫原

反应性数据

性能和储存信息

形式

纯度

存储溶液

运输条件

推荐的短期储存时间

推荐的短期储存条件

推荐的长期储存条件

分装信息

储存信息

产品实验方案

靶点信息

文献 (3)

Abcam Product Promise

Anti-DNA polymerase eta 抗体

Anti-DNA polymerase eta antibody

Immunocytochemistry/ Immunofluorescence - Anti-DNA polymerase eta antibody (AB236450)

Flow Cytometry - Anti-DNA polymerase eta antibody (AB236450)

Immunoprecipitation - Anti-DNA polymerase eta antibody (AB236450)

Western blot - Anti-DNA polymerase eta antibody (AB236450)

关键信息

宿主种属

克隆

亚型

不含载体蛋白

反应种属

应用

免疫原

替代产品

Primary Antibodies

Primary Antibodies

Primary Antibodies

搭配使用产品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

反应性数据

性能和储存信息

形式

纯度

存储溶液

运输条件

推荐的短期储存时间

推荐的短期储存条件

推荐的长期储存条件

分装信息

储存信息

产品实验方案

靶点信息

文献 (3)

Abcam Product Promise