Anti-DNA polymerase alpha/POLA抗体(ab176734)
Key features and details
- Rabbit polyclonal to DNA polymerase alpha/POLA
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-DNA polymerase alpha/POLA抗体
参阅全部 DNA polymerase alpha/POLA 一抗 -
描述
兔多克隆抗体to DNA polymerase alpha/POLA -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Rabbit, Horse, Chicken, Guinea pig, Dog, Non human primates
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免疫原
Synthetic peptide within Human DNA polymerase alpha/POLA aa 1175-1225. The exact sequence is proprietary. NP_058633.2.
Database link: P09884 -
阳性对照
- HeLa and 293T cell lysates
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, 99% Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab176734 is affinity purified using an epitope specific to DNA polymerase alpha/POLA immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab176734于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 166 kDa.
|
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| IP |
Use at 2-5 µg/mg of lysate.
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| 说明 |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 166 kDa. |
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IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes. -
序列相似性
Belongs to the DNA polymerase type-B family. -
翻译后修饰
A 165 kDa form is probably produced by proteolytic cleavage at Lys-124. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 418593 Chicken
- Entrez Gene: 480869 Dog
- Entrez Gene: 100717633 Guinea pig
- Entrez Gene: 100052225 Horse
- Entrez Gene: 5422 Human
- Entrez Gene: 100354472 Rabbit
- Omim: 312040 Human
- SwissProt: P09884 Human
see all -
别名
- DKFZp686K1672 antibody
- DNA polymerase alpha 1 catalytic subunit antibody
- DNA polymerase alpha catalytic subunit antibody
see all
图片
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Western blot - Anti-DNA polymerase alpha/POLA antibody (ab176734)All lanes : Anti-DNA polymerase alpha/POLA antibody (ab176734) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 166 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-DNA polymerase alpha/POLA antibody (ab176734)ab176734 at 0.4 µg/ml detecting DNA polymerase alpha/POLA in HeLa whole cell lysate by WB following IP. ab176734 was used for IP at 3 µg/mg of lysate, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 10 seconds.
数据表及文件
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Datasheet download
文献 (2)
ab176734 被引用在 2 文献中.
- Coulombe P et al. The ORC ubiquitin ligase OBI1 promotes DNA replication origin firing. Nat Commun 10:2426 (2019). PubMed: 31160578
- Moiseeva T et al. ATR kinase inhibition induces unscheduled origin firing through a Cdc7-dependent association between GINS and And-1. Nat Commun 8:1392 (2017). PubMed: 29123096
