重组Anti-DNA PKcs (phospho S2056)抗体[EPR5670] (ab124918)
![ChIC/CUT&RUN sequencing - Anti-DNA PKcs (phospho S2056) antibody [EPR5670] (ab124918)](https://www.abcam.cn/ps/products/124/ab124918/Images/ab124918-516650-antidnapkcsphosphos2056antibodyepr5670chiccutandrunsequencing.jpg "ChIC/CUT&RUN sequencing - Anti-DNA PKcs (phospho S2056) antibody [EPR5670] (ab124918)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5670] to DNA PKcs (phospho S2056)
- Suitable for: ICC/IF, WB, IHC-P, ChIC/CUT&RUN-seq, ELISA
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-DNA PKcs (phospho S2056)抗体[EPR5670]
参阅全部 DNA PKcs 一抗 -
描述
兔单克隆抗体[EPR5670] to DNA PKcs (phospho S2056) -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-P, ChIC/CUT&RUN-seq, ELISAmore details -
种属反应性
与反应: Human
不与反应: Mouse, Rat -
免疫原
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阳性对照
- WB: Jurkat whole cell lysate (ab7899). IHC-P: Human lung carcinoma and brain tissues. ICC/IF: Jurkat cells. ChIC/CUT&RUN-Seq: U2OS cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
解离常数（KD）
KD = 2.60 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5670 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124918于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/100 - 1/500.
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| WB | (1) |
1/5000 - 1/10000. Predicted molecular weight: 469 kDa.
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| IHC-P |
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| ChIC/CUT&RUN-seq |
Use at an assay dependent concentration.
5 µg |
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| ELISA |
Use a concentration of 0.001 - 1 µg/ml.
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| 说明 |
|---|
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ICC/IF
1/100 - 1/500. |
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WB
1/5000 - 1/10000. Predicted molecular weight: 469 kDa. |
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IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
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ChIC/CUT&RUN-seq
Use at an assay dependent concentration. 5 µg |
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ELISA
Use a concentration of 0.001 - 1 µg/ml. |
靶标
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功能
Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D. -
序列相似性
Belongs to the PI3/PI4-kinase family.
Contains 1 FAT domain.
Contains 1 FATC domain.
Contains 2 HEAT repeats.
Contains 1 PI3K/PI4K domain.
Contains 3 TPR repeats. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. Autophosphorylated on Thr-2609, Thr-2638 and Thr-2647. Thr-2609 is a DNA damage-inducible phosphorylation site (inducible with ionizing radiation, IR). Autophosphorylation induces a conformational change that leads to remodeling of the DNA-PK complex, requisite for efficient end processing and DNA repair.
S-nitrosylated by GAPDH. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 5591 Human
- Omim: 600899 Human
- SwissProt: P78527 Human
- Unigene: 491682 Human
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别名
- DNA dependent protein kinase catalytic subunit antibody
- DNA PK catalytic subunit antibody
- DNA-dependent protein kinase catalytic subunit antibody
see all
图片
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ChIC/CUT&RUN sequencing - Anti-DNA PKcs (phospho S2056) antibody [EPR5670] (ab124918)
ChIC/CUT&RUN was performed using a pAG-MNAse at a final concentration of 700 ng/mL, 2.5 x 10^5 U2OS cells treated with Etoposide (10μM 20h) and 5 µg of ab124918 [EPR5670]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown.
Additional screenshots of mapped reads can be downloaded here.
The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.
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![Western blot - Anti-DNA PKcs (phospho S2056) antibody [EPR5670] (ab124918)](https://www.abcam.cn/ps/products/124/ab124918/Images/ab124918-253939-anti-dna-pkcs-phospho-s2056-antibody-epr5670-western-blot.jpg)
Western blot - Anti-DNA PKcs (phospho S2056) antibody [EPR5670] (ab124918)All lanes : Anti-DNA PKcs (phospho S2056) antibody [EPR5670] (ab124918) at 1/10000 dilution (purified)
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate - untreated
Lane 2 : Jurkat whole cell lysate - treated with alkaline phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Predicted band size: 469 kDa
Observed band size: 469 kDaBlocking and dilution buffer: 5% NFDM/TBST.
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![Immunocytochemistry/ Immunofluorescence - Anti-DNA PKcs (phospho S2056) antibody [EPR5670] (ab124918)](https://www.abcam.cn/ps/products/124/ab124918/Images/ab124918-253869-anti-dna-pkcs-phospho-s2056-antibody-epr5670-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-DNA PKcs (phospho S2056) antibody [EPR5670] (ab124918)Immunocytochemistry/Immunofluorescence analysis of Jurkat cells (untreated and treated with Alkaline Phosphatase) labelling DNA PKcs (phospho S2056) with purified ab124918 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.
Control 1: primary antibody (1/500) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).
Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA PKcs (phospho S2056) antibody [EPR5670] (ab124918)](https://www.abcam.cn/ps/products/124/ab124918/Images/ab124918-253893-anti-dna-pkcs-phospho-s2056-antibody-epr5670-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA PKcs (phospho S2056) antibody [EPR5670] (ab124918)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling DNA PKcs (phospho S2056) with purified ab124918 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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![Western blot - Anti-DNA PKcs (phospho S2056) antibody [EPR5670] (ab124918)](https://www.abcam.cn/ps/products/124/ab124918/Images/ab124918-138920-anti-dna-pkcs-phospho-s2056-antibody-epr5670-western-blot.jpg)
Western blot - Anti-DNA PKcs (phospho S2056) antibody [EPR5670] (ab124918)All lanes : Anti-DNA PKcs (phospho S2056) antibody [EPR5670] (ab124918) at 1/1000 dilution (unpurified)
Lane 1 : Jurkat cell lysate, untreated
Lane 2 : Jurkat cell lysate, treated with alkaline phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 469 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA PKcs (phospho S2056) antibody [EPR5670] (ab124918)](https://www.abcam.cn/ps/products/124/ab124918/Images/ab124918-138919-anti-dna-pkcs-phospho-s2056-antibody-epr5670-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA PKcs (phospho S2056) antibody [EPR5670] (ab124918)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue labelling DNA PKcs (phospho S2056) with unpurified ab124918 at a dilution of 1/100.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![ELISA - Anti-DNA PKcs (phospho S2056) antibody [EPR5670] (ab124918)](https://www.abcam.cn/ps/products/124/ab124918/Images/ab124918-255533-anti-dna-pkcs-phospho-s2056-antibody-epr5670-elisa.jpg)
ELISA - Anti-DNA PKcs (phospho S2056) antibody [EPR5670] (ab124918)Serially diluted ab124918 was bound to immobilised phospho- or control peptides (1 microgram per mL). The antibody was detected by HRP-labelled goat anti-rabbit IgG (ab97080; diluted 50000 times) and signal was developed with TMB substrate.
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![OI-RD Scanning - Anti-DNA PKcs (phospho S2056) antibody [EPR5670] (ab124918)](https://www.abcam.cn/ps/products/124/ab124918/Images/ab124918-138918-anti-dna-pkcs-phospho-s2056-antibody-epr5670.jpg)
OI-RD Scanning - Anti-DNA PKcs (phospho S2056) antibody [EPR5670] (ab124918) -
![Anti-DNA PKcs (phospho S2056) antibody [EPR5670] (ab124918)](https://www.abcam.cn/ps/products/124/ab124918/Images/ab124918-8-benefits-of-recombinant-antibodies.png)
Anti-DNA PKcs (phospho S2056) antibody [EPR5670] (ab124918)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (42)
ab124918 被引用在 42 文献中.
- Mendoza-Munoz PL et al. Ku-DNA binding inhibitors modulate the DNA damage response in response to DNA double-strand breaks. NAR Cancer 5:zcad003 (2023). PubMed: 36755959
- Huang M et al. PARP1 negatively regulates transcription of BLM through its interaction with HSP90AB1 in prostate cancer. J Transl Med 21:445 (2023). PubMed: 37415147
- Zhang Q et al. APE1 promotes non-homologous end joining by initiating DNA double-strand break formation and decreasing ubiquitination of artemis following oxidative genotoxic stress. J Transl Med 21:183 (2023). PubMed: 36894994
- Dharanipragada P et al. Blocking Genomic Instability Prevents Acquired Resistance to MAPK Inhibitor Therapy in Melanoma. Cancer Discov 13:880-909 (2023). PubMed: 36700848
- Taffoni C et al. DNA damage repair kinase DNA-PK and cGAS synergize to induce cancer-related inflammation in glioblastoma. EMBO J 42:e111961 (2023). PubMed: 36574362