重组Anti-DN-7抗体[EPR5266] (ab133239)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5266] to DN-7
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-DN-7抗体[EPR5266]
参阅全部 DN-7 一抗 -
描述
兔单克隆抗体[EPR5266] to DN-7 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human DN-7 aa 200-300.
Database link: Q9HBM6 -
阳性对照
- 293T, MCF7, HeLa and A549 cell lysates. Human cervical carcinoma and tonsil tissues.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5266 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133239于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 28 kDa.
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 28 kDa. |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Essential for cell viability. TAF9 and TAF9B are involved in transcriptional activation as well as repression of distinct but overlapping sets of genes. May have a role in gene regulation associated with apoptosis. TAFs are components of the transcription factor IID (TFIID) complex, the TBP-free TAFII complex (TFTC), the PCAF histone acetylase complex and the STAGA transcription coactivator-HAT complex. TFIID or TFTC are essential for the regulation of RNA polymerase II-mediated transcription. -
序列相似性
Belongs to the TAF9 family. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 51616 Human
- Omim: 300754 Human
- SwissProt: Q9HBM6 Human
- Unigene: 592248 Human
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别名
- DN-7 antibody
- DN7 antibody
- Neuronal cell death related protein 7 antibody
see all
图片
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All lanes : Anti-DN-7 antibody [EPR5266] (ab133239) at 1/1000 dilution
Lane 1 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : A549 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti rabbit at 1/2000 dilution
Predicted band size: 28 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DN-7 antibody [EPR5266] (ab133239)
Immunohistochemical analysis of DN-7 in paraffin embedded Human cervical carcinoma tissue, using ab133239 at a 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DN-7 antibody [EPR5266] (ab133239)
Immunohistochemical analysis of DN-7 in paraffin embedded Human tonsil tissue, using ab133239 at a 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab133239 被引用在 2 文献中.
- Gu W et al. Downregulation of TAF9B by miR-7-5p Inhibits the Progression of Osteosarcoma. Onco Targets Ther 14:2917-2927 (2021). PubMed: 33958878
- Yu F et al. p53 Represses the Oncogenic Sno-MiR-28 Derived from a SnoRNA. PLoS One 10:e0129190 (2015). PubMed: 26061048