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Anti-DMPO Nitrone Adduct抗体[N1664A] (ab104902)

Submit a review Q&A (1)References (3)
Immunocytochemistry/ Immunofluorescence - Anti-DMPO Nitrone Adduct antibody [N1664A] (ab104902)
  • Western blot - Anti-DMPO Nitrone Adduct antibody [N1664A] (ab104902)

Key features and details

  • Mouse monoclonal [N1664A] to DMPO Nitrone Adduct
  • Suitable for: WB, ELISA, ICC/IF, IP
  • Reacts with: Species independent
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

FITC

概述

  • 产品名称

    Anti-DMPO Nitrone Adduct抗体[N1664A]
    参阅全部 DMPO Nitrone Adduct 一抗

  • 描述

    小鼠单克隆抗体[N1664A] to DMPO Nitrone Adduct

  • 宿主

    Mouse

  • 特异性

    Detects ~90kDa. Recognizes DMPO, DMPO-octanoic acid, DMPO-protein adducts and DMPO-DNA adducts. Does not cross-react with non-adducted proteins or DNA.

  • 经测试应用

    适用于: WB, ELISA, ICC/IF, IPmore details

  • 种属反应性

    与反应: Species independent

  • 免疫原

    5,5-dimethyl-2-(8-octanoic acid)-1-pyrrolone-N-oxide conjugated to Ovalbumin.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab104902于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use a concentration of 1 - 10 µg/ml.
ELISA
Use a concentration of 1 - 10 µg/ml.
ICC/IF
Use a concentration of 1 - 10 µg/ml.
IP
Use at an assay dependent dilution. Follow the method described by Chatterjee, S. et. al. PubMed 19049863.
说明
WB
Use a concentration of 1 - 10 µg/ml.
ELISA
Use a concentration of 1 - 10 µg/ml.
ICC/IF
Use a concentration of 1 - 10 µg/ml.
IP
Use at an assay dependent dilution. Follow the method described by Chatterjee, S. et. al. PubMed 19049863.

靶标

  • 相关性

    For measurement of protein radicals; some proteins with endogenous peroxidase activity are especially susceptible to forming DMPO adducts.

  • 别名

    • 55 dimethyl 1 pyrroline N oxide nitrone adduct antibody

图片

  • Immunocytochemistry/ Immunofluorescence - Anti-DMPO Nitrone Adduct antibody [N1664A] (ab104902)
    Immunocytochemistry/ Immunofluorescence - Anti-DMPO Nitrone Adduct antibody [N1664A] (ab104902)

    Immuncytochemistry/Immunofluorescence analysis of Mouse macrophage cells labelling DMPO Nitrone Adduct with ab104902 at 10μg/mL. Left - with DMPO, Right - no DMPO. Top to bottom - DMPO, DAPI, overlay.

  • Western blot - Anti-DMPO Nitrone Adduct antibody [N1664A] (ab104902)
    Western blot - Anti-DMPO Nitrone Adduct antibody [N1664A] (ab104902)
    All lanes : Anti-DMPO Nitrone Adduct antibody [N1664A] (ab104902) at 10 µg/ml

    Lane 1 : 10µM Hb
    Lane 2 : 10µM Hb + 100µM HOCL
    Lane 3 : 10µM Hb + 500µM HOCL
    Lane 4 : 10µM Hb + 1000µM HOCL
    Lane 5 : 10µM Hb with 20mM DMPO
    Lane 6 : 10µM Hb + 100µM HOCL with 20mM DMPO
    Lane 7 : 10µM Hb + 500µM HOCL with 20mM DMPO
    Lane 8 : 10µM Hb + 1000µM HOCL with 20mM DMPO
    Lane 9 : 10µM Hb with 100mM DMPO
    Lane 10 : 10µM Hb + 100µM HOCL with 100mM DMPO
    Lane 11 : 10µM Hb + 500µM HOCL with 100mM DMPO
    Lane 12 : 10µM Hb + 1000µM HOCL with 100mM DMPO

文献 (3)

发表研究结果有使用 ab104902？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab104902 被引用在 3 文献中.

  • Chen T  et al. Edonerpic maleate regulates glutamate receptors through CRMP2- and Arc-mediated mechanisms in response to brain trauma. Cell Death Discov 8:95 (2022). PubMed: 35246523
  • Lozoya OA  et al. Single Nucleotide Resolution Analysis Reveals Pervasive, Long-Lasting DNA Methylation Changes by Developmental Exposure to a Mitochondrial Toxicant. Cell Rep 32:108131 (2020). PubMed: 32937126
  • Sun L  et al. Targeted DNA damage at individual telomeres disrupts their integrity and triggers cell death. Nucleic Acids Res 43:6334-47 (2015). PubMed: 26082495

客户评价及客户问答

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Question


I would like to know if this above antitbody is tagged with a fluorescence molecule so that it can be followed in cells or small animals by a fluorescence measuring device. If not, can you make it in a fluorescence tagged form?

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Answer



Unfortunately we do not design custom made antibodies. This particular one does not have a fluorescent molecule attached to it and requires a secondary for detection.

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