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AB104902

Anti-DMPO Nitrone Adduct抗体[N1664A]

Anti-DMPO Nitrone Adduct antibody [N1664A]

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(4 Publications)

Mouse Monoclonal DMPO Nitrone Adduct antibody. Suitable for WB, IP, ELISA, ICC/IF and reacts with Chemical samples. Cited in 4 publications. Immunogen corresponding to Chemical / Small Molecule corresponding to DMPO Nitrone Adduct.

查看别名

55 dimethyl 1 pyrroline N oxide nitrone adduct

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-DMPO Nitrone Adduct antibody [N1664A] (AB104902)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DMPO Nitrone Adduct antibody [N1664A] (AB104902)

Immuncytochemistry/Immunofluorescence analysis of Mouse macrophage cells labelling DMPO Nitrone Adduct with ab104902 at 10μg/mL. Left - with DMPO, Right - no DMPO. Top to bottom - DMPO, DAPI, overlay.

不同偶联物与剂型 (1)

  • 519 FITC

    FITC Anti-DMPO Nitrone Adduct antibody [N1664A]

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

N1664A

亚型

IgG1

不含载体蛋白

No

应用

ICC/IF, IP, WB, ELISA

applications

特异性

<p>Detects ~90kDa. Recognizes DMPO, DMPO-octanoic acid, DMPO-protein adducts and DMPO-DNA adducts. Does not cross-react with non-adducted proteins or DNA.</p>

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替代产品

Primary Antibodies

AB23702

Anti-DMPO Nitrone Adduct antibody

Western blot - Anti-DMPO Nitrone Adduct antibody (AB23702)

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搭配使用产品

Assay Kits

AB110217

MitoBiogenesis™ In-Cell ELISA Kit (Colorimetric)

In-Cell ELISA - MitoBiogenesis™ In-Cell ELISA Kit (Colorimetric) (AB110217)

  • 5
  • 2
Assay Kits

AB133085

Lipid Hydroperoxide (LPO) Assay Kit

Functional Studies - Lipid Hydroperoxide (LPO) Assay Kit (AB133085)

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Assay Kits

AB233470

Peroxynitrite Assay Kit (Cell-based, Flow cytometry)

Flow Cytometry - Peroxynitrite Assay Kit (Cell-based, Flow cytometry) (AB233470)

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Assay Kits

AB233468

Peroxynitrite Assay Kit (Cell-based)

Functional Studies - Peroxynitrite Assay Kit (Cell-based) (AB233468)

  • 0
  • 0
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-10 µg/mL", "WB-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>Follow the method described by Chatterjee, S. <em>et. al. </em> PubMed 19049863.</p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "1-10 µg/mL", "ELISA-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein G
存储溶液
pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The DMPO nitrone adduct also known as 55-Dimethyl-1-Pyrroline N-Oxide adduct plays an important role in trapping free radicals during biological reactions. As a spin trap DMPO captures highly reactive radicals like hydroxyl and superoxide radicals converting them to more stable radical adducts that can be detected by Electron Spin Resonance (ESR) spectroscopy. The mass of the DMPO nitrone adduct varies depending on the specific radical bound but the core structure of DMPO nitrone has a molecular weight of 113.15 g/mol. DMPO adducts are expressed in conditioned systems and are especially useful in detecting radical species in cellular environments.
Biological function summary

DMPO nitrone adduct formation is critical for investigating oxidative stress within cells. It does not form part of a complex but acts as an independent trap for free radicals. By stabilizing reactive radicals DMPO adducts help to provide a clearer picture of the radical landscape within biological systems. This insight is essential for understanding the balance between reactive oxygen species production and antioxidant defense mechanisms in various cell types and tissues.

Pathways

DMPO nitrone adduct formation links closely to pathways involving oxidative stress and redox signaling. In these pathways DMPO assists in monitoring dynamic changes and interactions between radicals and antioxidant proteins like superoxide dismutase (SOD) and catalase. These pathways are important for maintaining cellular health and preventing oxidative damage.

The use of DMPO nitrone adduct is significant for research on oxidative stress-related conditions. It is connected to diseases like Alzheimer's and cardiovascular disorders where oxidative stress and free radical damage play a well-documented role. The formation of DMPO adducts provides a method for assessing the extent of radical involvement offering insights into disease mechanisms. The interaction between DMPO and proteins such as amyloid-beta in Alzheimer's highlights the importance of free radical monitoring in understanding disease progression.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

See full target information DMPO Nitrone Adduct
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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Redox biology 63:102733 PubMed37172395

2023

Temporary alteration of neuronal network communication is a protective response to redox imbalance that requires GPI-anchored prion protein.

Applications

Unspecified application

Species

Unspecified reactive species

Simote T Foliaki,Aleksandar Wood,Katie Williams,Anna Smith,Ryan O Walters,Chase Baune,Bradley R Groveman,Cathryn L Haigh

Cell death discovery 8:95 PubMed35246523

2022

Edonerpic maleate regulates glutamate receptors through CRMP2- and Arc-mediated mechanisms in response to brain trauma.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Chen,Li-Kun Yang,Pu Ai,Jie Zhu,Chun-Hua Hang,Yu-Hai Wang

Cell reports 32:108131 PubMed32937126

2020

Single Nucleotide Resolution Analysis Reveals Pervasive, Long-Lasting DNA Methylation Changes by Developmental Exposure to a Mitochondrial Toxicant.

Applications

Unspecified application

Species

Unspecified reactive species

Oswaldo A Lozoya,Fuhua Xu,Dagoberto Grenet,Tianyuan Wang,Sara A Grimm,Veronica Godfrey,Suramya Waidyanatha,Richard P Woychik,Janine H Santos

Nucleic acids research 43:6334-47 PubMed26082495

2015

Targeted DNA damage at individual telomeres disrupts their integrity and triggers cell death.

Applications

Unspecified application

Species

Unspecified reactive species

Luxi Sun,Rong Tan,Jianquan Xu,Justin LaFace,Ying Gao,Yanchun Xiao,Myriam Attar,Carola Neumann,Guo-Min Li,Bing Su,Yang Liu,Satoshi Nakajima,Arthur S Levine,Li Lan
View all publications
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