重组Anti-Dlx4/BP1抗体[EPR9263] (ab175218)
![Western blot - Anti-Dlx4/BP1 antibody [EPR9263] (ab175218)](https://www.abcam.cn/ps/products/175/ab175218/Images/ab175218-201557-anti-dlx4-antibody-epr9263-western-blot.jpg "Western blot - Anti-Dlx4/BP1 antibody [EPR9263] (ab175218)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9263] to Dlx4/BP1
- Suitable for: ICC/IF, IP, IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Dlx4/BP1抗体[EPR9263]
参阅全部 Dlx4/BP1 一抗 -
描述
兔单克隆抗体[EPR9263] to Dlx4/BP1 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IP, IHC-P, WBmore details
不适用于: Flow Cyt -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- SW480, K562 and Human placenta lysates; Human placenta and prostatic hyperplasia tissue; SW480 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR9263 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab175218于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/100 - 1/250.
|
|
| IP |
1/10 - 1/100.
|
|
| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 26 kDa.
|
| 说明 |
|---|
|
ICC/IF
1/100 - 1/250. |
|
IP
1/10 - 1/100. |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
1/1000 - 1/5000. Predicted molecular weight: 26 kDa. |
靶标
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功能
May play a role in determining the production of hemoglobin S. May act as a repressor. -
组织特异性
Expressed in leukemia cells and placenta. Also expressed in kidney and fetal liver. -
序列相似性
Belongs to the distal-less homeobox family.
Contains 1 homeobox DNA-binding domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 1748 Human
- Entrez Gene: 13394 Mouse
- Entrez Gene: 303469 Rat
- Omim: 601911 Human
- SwissProt: Q92988 Human
- SwissProt: P70436 Mouse
- Unigene: 591167 Human
- Unigene: 439766 Mouse
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别名
- Beta protein 1 antibody
- BP1 antibody
- Distal less homeo box 7 antibody
see all
图片
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Western blot - Anti-Dlx4/BP1 antibody [EPR9263] (ab175218)All lanes : Anti-Dlx4/BP1 antibody [EPR9263] (ab175218) at 1/1000 dilution
Lane 1 : SW480 cell lysate
Lane 2 : K562 cell lysate
Lane 3 : Human placenta tissue lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 26 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dlx4/BP1 antibody [EPR9263] (ab175218)](https://www.abcam.cn/ps/products/175/ab175218/Images/ab175218-201562-anti-dlx4-antibody-epr9263-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dlx4/BP1 antibody [EPR9263] (ab175218)Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue labeling Dlx4/BP1 with ab175218 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dlx4/BP1 antibody [EPR9263] (ab175218)](https://www.abcam.cn/ps/products/175/ab175218/Images/ab175218-201561-anti-dlx4-antibody-epr9263-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dlx4/BP1 antibody [EPR9263] (ab175218)Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling Dlx4/BP1 with ab175218 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-Dlx4/BP1 antibody [EPR9263] (ab175218)](https://www.abcam.cn/ps/products/175/ab175218/Images/ab175218-201560-anti-dlx4-antibody-epr9263-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Dlx4/BP1 antibody [EPR9263] (ab175218)Immunofluorescence analysis of SW480 cells labeling Dlx4/BP1 with ab175218 at 1/100 dilution.
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![Immunoprecipitation - Anti-Dlx4/BP1 antibody [EPR9263] (ab175218)](https://www.abcam.cn/ps/products/175/ab175218/Images/ab175218-201559-anti-dlx4-antibody-epr9263-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Dlx4/BP1 antibody [EPR9263] (ab175218)Western blot analysis on immunoprecipitation pellet from K562 cell lysate using ab175218 at 1/10 dilution.
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![Anti-Dlx4/BP1 antibody [EPR9263] (ab175218)](https://www.abcam.cn/ps/products/175/ab175218/Images/ab175218-6-benefits-of-recombinant-antibodies.png)
Anti-Dlx4/BP1 antibody [EPR9263] (ab175218)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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