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Anti-DLST抗体[9F4BD5] (ab110306)

Reviews (3) Submit a question References (2)
Immunocytochemistry/ Immunofluorescence - Anti-DLST antibody [9F4BD5] (ab110306)
  • Flow Cytometry - Anti-DLST antibody [9F4BD5] (ab110306)
  • Immunocytochemistry/ Immunofluorescence - Anti-DLST antibody [9F4BD5] (ab110306)
  • Flow Cytometry - Anti-DLST antibody [9F4BD5] (ab110306)

Key features and details

  • Mouse monoclonal [9F4BD5] to DLST
  • Suitable for: ICC/IF, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

概述

  • 产品名称

    Anti-DLST抗体[9F4BD5]
    参阅全部 DLST 一抗

  • 描述

    小鼠单克隆抗体[9F4BD5] to DLST

  • 宿主

    Mouse

  • 经测试应用

    适用于: ICC/IF, Flow Cytmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Full length native protein (purified). This information is considered to be commercially sensitive.

  • 阳性对照

    • Human HDFn and HL-60 cells

  • 常规说明

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Product was previously marketed under the MitoSciences sub-brand.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab110306于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF (1)
Use a concentration of 1 µg/ml.
Flow Cyt (1)
Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
说明
ICC/IF
Use a concentration of 1 µg/ml.
Flow Cyt
Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能

    The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3).

  • 通路

    Amino-acid degradation; L-lysine degradation via saccharopine pathway; glutaryl-CoA from L-lysine: step 6/6.

  • 序列相似性

    Belongs to the 2-oxoacid dehydrogenase family.
    Contains 1 lipoyl-binding domain.

  • 细胞定位

    Mitochondrion.
  • Information by UniProt

  • 数据库链接

    • 别名

      • 2-oxoglutarate dehydrogenase complex component E2 antibody
      • Dihydrolipoamide S succinyltransferase (E2 component of 2 oxo glutarate complex) antibody
      • Dihydrolipoamide S succinyltransferase antibody
      • Dihydrolipoamide succinyltransferase component of 2 oxoglutarate dehydrogenase complex antibody
      • Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex antibody
      • Dihydrolipoyllysine residue succinyltransferase component of 2 oxoglutarate dehydrogenase complex antibody
      • Dihydrolipoyllysine residue succinyltransferase component of 2 oxoglutarate dehydrogenase complex mitochondrial antibody
      • Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex antibody
      • Dlst antibody
      • DLTS antibody
      • E2 antibody
      • E2K antibody
      • mitochondrial antibody
      • ODO2_HUMAN antibody
      • OGDC-E2 antibody
      see all

    图片

    • Immunocytochemistry/ Immunofluorescence - Anti-DLST antibody [9F4BD5] (ab110306)
      Immunocytochemistry/ Immunofluorescence - Anti-DLST antibody [9F4BD5] (ab110306)
      Immunocytochemistry image of ab110306-stained Human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min). Cells were then incubated with ab110306 at 1 µg/ml for 2 h at room temperature or over night at 4°C. The secondary antibody was (green) Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in mitochondria.
    • Flow Cytometry - Anti-DLST antibody [9F4BD5] (ab110306)
      Flow Cytometry - Anti-DLST antibody [9F4BD5] (ab110306)
      HL-60 cells were stained with 1 µg/mL ab110306 (blue) or an equal amount of an isotype control antibody (red) and analyzed by Flow Cytometry.
    • Immunocytochemistry/ Immunofluorescence - Anti-DLST antibody [9F4BD5] (ab110306)
      Immunocytochemistry/ Immunofluorescence - Anti-DLST antibody [9F4BD5] (ab110306)This image is courtesy of an anonymous Abreview
      ab110306 staining DLST in Human monocyte-derived macrophages by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 0.1% and blocked with 5% Goat serum for 60 minutes at 21°C. Samples were incubated with primary antibody (1/1000 in PBS + 1% BSA) for 12 hours at 4°C. A Cy2®-conjugated Goat anti-mouse IgG polyclonal was used as the secondary antibody.

      See Abreview

    • Flow Cytometry - Anti-DLST antibody [9F4BD5] (ab110306)
      Flow Cytometry - Anti-DLST antibody [9F4BD5] (ab110306)This image is courtesy of an anonymous Abreview
      ab110306 staining DLST in Human monocyte-derived macrophages by Flow Cytometry. Cells were prepared by scraoing in PBS and fixation by paraformaldehyde. The sample was incubated with the primary antibody (1/1000 in PBS + 1% BSA) for 60 minutes at 4°C. An Alexa Fluor®488-conjugated Goat anti-mouse IgG polyclonal(1/100) was used as the secondary antibody.
      Gating Strategy: Dead cells excluded.

      See Abreview

    文献 (2)

    发表研究结果有使用 ab110306？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab110306 被引用在 2 文献中.

    • Leandro J  et al. DHTKD1 and OGDH display substrate overlap in cultured cells and form a hybrid 2-oxo acid dehydrogenase complex in vivo. Hum Mol Genet 29:1168-1179 (2020). PubMed: 32160276
    • Malty RH  et al. A Map of Human Mitochondrial Protein Interactions Linked to Neurodegeneration Reveals New Mechanisms of Redox Homeostasis and NF-?B Signaling. Cell Syst 5:564-577.e12 (2017). PubMed: 29128334

    客户评价及客户问答

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    Flow Cytometry abreview for Anti-DLST antibody [9F4BD5 ]

     Good
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (human MDM)
    Specification
    human MDM
    Preparation
    Cell harvesting/tissue preparation method: SCRAPING
    Sample buffer: PBS
    Fixation
    Paraformaldehyde
    Permeabilization
    No
    Gating Strategy
    dead cells were excluded
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Feb 28 2013

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