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Anti-DLL1抗体(ab10554)

  • Datasheet
  • SDS
Reviews (2)Q&A (4)References (24)

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Immunocytochemistry/ Immunofluorescence - Anti-DLL1 antibody (ab10554)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLL1 antibody (ab10554)
  • Immunocytochemistry/ Immunofluorescence - Anti-DLL1 antibody (ab10554)
  • Western blot - Anti-DLL1 antibody (ab10554)

Key features and details

  • Rabbit polyclonal to DLL1
  • Suitable for: IHC-P, WB, ICC/IF, IHC-Fr, IHC-FoFr, ELISA
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-DLL1抗体
    参阅全部 DLL1 一抗
  • 描述

    兔多克隆抗体to DLL1
  • 宿主

    Rabbit
  • 特异性

    Activity against Delta-1 from other species has not been determined but is expected based on sequence homology. Expected to cross-react with rat, human and chicken.

  • 经测试应用

    适用于: IHC-P, WB, ICC/IF, IHC-Fr, IHC-FoFr, ELISAmore details
  • 种属反应性

    与反应: Mouse, Rat, Human
    预测可用于: Chicken
  • 免疫原

    Synthetic peptide:

    CGEEWSQDLHSSGRTDLRYS

    conjugated to KLH, corresponding to amino acids 155-173 of Mouse Delta-1.
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride
  • Concentration information loading...
  • 纯度

    Affinity purified
  • 纯化说明

    Anti-DELTA-1 Antibody was affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Stem Cells
    • Embryonic Stem Cells
    • Surface Molecules
    • Stem Cells
    • Hematopoietic Progenitors
    • Hematopoietic Stem Cells
    • Human Lineage Negative
    • Stem Cells
    • Signaling Pathways
    • Notch
    • Surface Molecules
    • Stem Cells
    • Hematopoietic Progenitors
    • Surface Molecules
    • Developmental Biology
    • Embryogenesis
    • Somatogenesis
    • Developmental Biology
    • Embryogenesis
    • Embryonic stem cells
    • Intracellular

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab10554于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P (1)
Use at an assay dependent concentration.
WB
1/100 - 1/1000. Predicted molecular weight: 78 kDa.
ICC/IF
Use at an assay dependent concentration.
IHC-Fr (1)
Use at an assay dependent concentration.
IHC-FoFr
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
说明
IHC-P
Use at an assay dependent concentration.
WB
1/100 - 1/1000. Predicted molecular weight: 78 kDa.
ICC/IF
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
IHC-FoFr
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.

靶标

  • 功能

    Acts as a ligand for Notch receptors. Blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor.
  • 组织特异性

    Expressed in heart and pancreas, with lower expression in brain and muscle and almost no expression in placenta, lung, liver and kidney.
  • 序列相似性

    Contains 1 DSL domain.
    Contains 8 EGF-like domains.
  • 翻译后修饰

    Ubiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation.
  • 细胞定位

    Membrane.
  • Target information above from: UniProt accession O00548 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 28514 Human
    • Entrez Gene: 13388 Mouse
    • Entrez Gene: 84010 Rat
    • Omim: 606582 Human
    • SwissProt: O00548 Human
    • SwissProt: Q61483 Mouse
    • SwissProt: P97677 Rat
    • Unigene: 379912 Human
    • Unigene: 4875 Mouse
    • Unigene: 10628 Rat
    see all
  • 别名

    • Delta (Drosophila) like 1 antibody
    • Delta antibody
    • Delta like 1 (Drosophila) antibody
    • Delta like 1 antibody
    • Delta like 1 protein antibody
    • Delta like protein 1 antibody
    • Delta like protein 1 precursor antibody
    • Delta, Drosophila, homolog of 1 antibody
    • Delta-like protein 1 antibody
    • Delta1 antibody
    • DL1 antibody
    • DLL 1 antibody
    • DLL1 antibody
    • DLL1_HUMAN antibody
    • Drosophila Delta homolog 1 antibody
    • H-Delta-1 antibody
    • OTTHUMP00000017690 antibody
    see all

图片

  • Immunocytochemistry/ Immunofluorescence - Anti-DLL1 antibody (ab10554)
    Immunocytochemistry/ Immunofluorescence - Anti-DLL1 antibody (ab10554)Greene M et al., PLos One, 2016 Sep 13;11(9):e0161157. Fig 1.; doi: 10.1371/journal.pone.0161157 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    ab10554 staining DLL1 in U2OS cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde, permeabilized with 0.3% Triton X-100 in PBS and blocked with 4% BSA for 1 hour. Samples were incubated with primary antibody for 1 hour at room temperature. DAPI used to stain nuclei

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLL1 antibody (ab10554)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLL1 antibody (ab10554)

    ab10554 staining DLL1 in Mouse embryonic kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Samples were incubated with primary antibody (2ug/ml) for 1.5 hours at room temperature. A peroxidase-conjugated polyclonal was used as the secondary antibody. Nuclei stained purple with hematoxylin.

  • Immunocytochemistry/ Immunofluorescence - Anti-DLL1 antibody (ab10554)
    Immunocytochemistry/ Immunofluorescence - Anti-DLL1 antibody (ab10554)
    ICC/IF image of ab10554 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10554, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Western blot - Anti-DLL1 antibody (ab10554)
    Western blot - Anti-DLL1 antibody (ab10554)

    Western blot using Delta 1 antibody (ab10554) at 1/500 tested on the following tissue lysates:

    Lane 1: Mouse Heart
    Lane 2: Mouse Pancreas
    Lane 3: Mouse Brain
    Lane 4: Rat Heart
    Lane 5: Rat Pancreas
    Lane 6: Rat Brain

    Secondary ab: Goat anti-rabbit IgG ab6721 (1/5000)
    Exposure time: 1 minute
    Expected molecular weight: 77kDa
    Cell lysates at 20µg per lane.

    NB: This image shows that is was not possible to detect Delta 1 immunoreactivity by WB in the lysates tested. However little is known about Delta 1 expression and it is likely to be developmentally regulated with low expression/abundance making it difficult to detect in whole tissue homogenates. In favour of Delta 1 antibody (ab10554), the staining on the WB shows very low background and little cross reactivity in whole homogenates.

    Western blot using Delta 1 antibody (ab10554) at 1/500 tested on the following tissue lysates:

    Lane 1: Mouse Heart

实验方案

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (24)

发表研究结果有使用 ab10554?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab10554 被引用在 24 文献中.

  • Wu C  et al. B Lymphocytes Balance Th1/Th2 to Participate in Fibrotic Hypersensitivity Pneumonia Induced by Pigeon Shedding. Int Arch Allergy Immunol 184:122-131 (2023). PubMed: 36323228
  • Cotellessa L  et al. Defective jagged-1 signaling affects GnRH development and contributes to congenital hypogonadotropic hypogonadism. JCI Insight 8:N/A (2023). PubMed: 36729644
  • Wang Z  et al. Kindlin-2 in myoepithelium controls luminal progenitor commitment to alveoli in mouse mammary gland. Cell Death Dis 14:675 (2023). PubMed: 37833248
  • Liu CF  et al. Whole-Transcriptome Profiling of Human Heart Tissues Reveals the Potential Novel Players and Regulatory Networks in Different Cardiomyopathy Subtypes of Heart Failure. Circ Genom Precis Med 14:e003142 (2021). PubMed: 33517678
  • Zhang Q  et al. Single-cell analysis reveals dynamic changes of neural cells in developing human spinal cord. EMBO Rep 22:e52728 (2021). PubMed: 34605607
View all Publications for this product

客户评价及客户问答

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1-6 of 6 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-DLL1 antibody

Average
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cow Tissue sections (Liver)
Specification
Liver
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization
Yes - Triton
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Daniel Murta

Verified customer

提交于 May 15 2013

Immunohistochemistry (Frozen sections) abreview for Anti-DLL1 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Intestine)
Specification
Intestine
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% Triton 20 min at RT
Blocking step
Protein Block as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Adam Gracz

Verified customer

提交于 Jan 04 2013

Question

I am interested in the product ab10554. it the mouse DL1 Ab. however, the datasheet isn't nice. I am sure that mouse spleen express high levels of DL1. I am wondering if you have done it or not. I would like to know the result before I order it. thank you very much,

Read More

Abcam community

Verified customer

Asked on Nov 17 2005

Answer

Thank you for your email. Ab10554 is a Fast-Track antibody - Fast-Track antibodies are immunogen affinity purified and are shown by ELISA to have high titre values against their immunogen. We are currently testing them for use in Western blotting. At this stage, there can be delays. We sometimes experience problems in searching for / developing the correct lysate or knock-out cell-line. In addition, certain products can be difficult to characterise, due, for instance, to low expression levels or a lack of expression in easily available lysates. The preliminary characterization of this antibody has shown the following: by Western blotting (please see image on the online datasheet) it was not possible to detect Delta 1 immunoreactivity by WB in the rat and mouse lysates tested (brain, pancreas and heart). However little is known about Delta 1 expression and it is likely to be developmentally regulated with low expression/abundance making it difficult to detect in whole tissue homogenates. Mouse spleen has not been tested to our knowledge with this antibody. Please note that Fast-Track antibodies are released early with the following understanding: As Fast-Track antibodies are not yet fully characterised we are unable to offer our normal level of technical support. We cannot guarantee that the antibody will work in any application other than ELISA against the immunizing peptide and therefore we are unable to offer a refund if the antibody does not work in your application. However, Fast-Track antibodies are offered at a reduced price. We would greatly appreciate your feedback on these antibodies, whether positive or negative. We will award 1,000 Abpoints to the first researcher who sends us positive feedback (including an image and details of materials & methods). Conclusive negative feedback that leads us to withdraw the antibody will also be rewarded with 1,000 Abpoints. Other useful positive and negative feedback will be rewarded with 50 Abpoints. All awards are subject to approval by Abcam scientists and provision of suitable data. Please submit feedback to fast-track@abcam.com Please contact us again if you have any additional questions.

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Abcam Scientific Support

回复于 Nov 17 2005

Question

I would like to know if it is possible to get the pre-immune serum of the same rabbit that the one that has been immunized to give antibodies raised against the targeted protein. The antibodies that I'm refering to are: ab7280 Lot. GR17350 ab8926 Lot. GR21934 ab10554 Lot. GR4787 ab23426 Lot. GR15959 ab27526 Lot. GR40329

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Abcam community

Verified customer

Asked on Apr 15 2013

Answer


Unfortunately it is not possible for us to sell the pre-immune serum of our rabbits.
I am sorry that on this occasion I could not be of more help.

Read More

Abcam Scientific Support

回复于 Apr 15 2013

Question

I would like to try this antibody in IHC. It hasn't been tested yet. could you please, send me a free small aliquot, or should I purchase an entire tube? Thanks for your answer.

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Abcam community

Verified customer

Asked on Jul 28 2005

Answer

This Rabbit polyclonal antibody to Delta 1 has been shown to work in ELISA against the peptide immunogen but has not been shown to detect the endogenous protein (WB) in the lysates specified on the datsheet. The anitbody is sold as a special investigate grade called "Fast Track" and is offered at a reduced price (EUR 215 versus EUR 330). We explain the terms and conditions associated with fast-track antibodies on our datasheet. Furthermore we offer a sustantial number of reward points (currently 1000) to researchers who get back to us with positive or negative information about the antibody. So, there is no guarantee that this antibody will work in IHC and we do not offer free trial sized samples, but we believe that it is a sufficiently novel antibody to offer to the research community under the terms described above and in further detail on the datasheet.

Read More

Abcam Scientific Support

回复于 Jul 29 2005

Question

Customer is interested in using ab10554 in confocal microscopy. He's interested in using ab6717 and ab6793 as the secondary antibodies. How long approximately will the fluorescent signal last?

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Abcam community

Verified customer

Asked on Jul 01 2005

Answer

Thank you for your enquiry. We cannot give you an exact time before which the signal will fade with these fluorophore conjugated secondary antibodies. The signal could last a week (the time frame that you mentioned that you would need) if your samples are prepared and stored under the correct conditions (at 4C in the dark). However, we do not know this for sure. Also, some fluorophores will fade faster than others. Please note that ab10554 has not yet been tested for application in confocal microscopy. If you have any additional questions, please contact us again.

Read More

Abcam Scientific Support

回复于 Jul 05 2005

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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