重组Anti-DLK-1抗体[EPR19830] (ab210471)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19830] to DLK-1
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat
Related conjugates and formulations
概述
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产品名称
Anti-DLK-1抗体[EPR19830]
参阅全部 DLK-1 一抗 -
描述
兔单克隆抗体[EPR19830] to DLK-1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Mouse placenta and embryo lysates. IHC-P: Mouse cerebrum and E14.5 embryo tissues; Rat cerebrum tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR19830 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab210471于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (1) |
1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 41 kDa).
|
IHC-P | (2) |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
说明 |
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WB
1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 41 kDa). |
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
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功能
May have a role in neuroendocrine differentiation. -
组织特异性
Found within the stromal cells in close contact to the vascular structure of placental villi, yolk sac, fetal liver, adrenal cortex and pancreas and in the beta cells of the islets of Langerhans in the adult pancreas. Found also in some forms of neuroendocrine lung tumor tissue. -
序列相似性
Contains 6 EGF-like domains. -
翻译后修饰
N- and O-glycosylated. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 13386 Mouse
- Entrez Gene: 114587 Rat
- SwissProt: Q09163 Mouse
- Unigene: 157069 Mouse
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别名
- Adipocyte differentiation inhibitor protein antibody
- Brevideltin antibody
- Delta like 1 homolog antibody
see all
图片
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All lanes : Anti-DLK-1 antibody [EPR19830] (ab210471) at 1/1000 dilution
Lane 1 : Mouse placenta lysate
Lane 2 : DLK-1-knockout mouse placenta lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 41 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 32 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The expression pattern is consistent with the literature (PMID: 21419176 and PMID: 19247431).
The tissue lysates were kindly provided by our collaborator Prof. Anne Ferguson-Smith, Cambridge University.
The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.
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Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling DLK-1 with ab210471 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in the substantia nigra and ventral tegmental area (arrow) of mouse cerebrum (PMID: 19515692) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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All lanes : Anti-DLK-1 antibody [EPR19830] (ab210471) at 1/5000 dilution
Lane 1 : Mouse placenta lysate at 0.005 µg
Lane 2 : Mouse embryo lysate at 5 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 41 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteBlocking/Dilution buffer: 5% NFDM/TBST.
The expression pattern is consistent with the literature (PMID: 21419176 and PMID: 19247431).
The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.
-
Immunohistochemical analysis of paraffin-embedded mouse E14.5 embryo tissue labeling DLK-1 with ab210471 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in mouse E14.5 embryo (PMID: 16456855) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
-
Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling DLK-1 with ab210471 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in the substantia nigra and ventral tegmental area (arrow) of rat cerebrum (PMID: 19515692) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (4)
ab210471 被引用在 4 文献中.
- Tian G et al. CpG site hypomethylation at ETS1-binding region regulates DLK1 expression in Chinese patients with Tetralogy of Fallot. Mol Med Rep 25:N/A (2022). IHC-P ; Human . PubMed: 35059744
- Han X et al. Deletion of Meg8-DMR Enhances Migration and Invasion of MLTC-1 Depending on the CTCF Binding Sites. Int J Mol Sci 23:N/A (2022). PubMed: 35955961
- Tian P et al. MicroRNA-127 Inhibits the Progression of Melanoma by Downregulating Delta-Like Homologue 1. Biomed Res Int 2020:8523465 (2020). WB ; Human . PubMed: 32051829
- Chen Y et al. Functional characterization of DLK1/MEG3 locus on chromosome 14q32.2 reveals the differentiation of pituitary neuroendocrine tumors. Aging (Albany NY) 13:1422-1439 (2020). PubMed: 33472171