Anti-DLK-1抗体[3A10] (ab119930)
Key features and details
- Mouse monoclonal [3A10] to DLK-1
- Suitable for: WB, ELISA, Flow Cyt, ICC/IF
- Reacts with: Mouse, Human, Recombinant fragment
- Isotype: IgG1
概述
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产品名称
Anti-DLK-1抗体[3A10]
参阅全部 DLK-1 一抗 -
描述
小鼠单克隆抗体[3A10] to DLK-1 -
宿主
Mouse -
经测试应用
适用于: WB, ELISA, Flow Cyt, ICC/IFmore details -
种属反应性
与反应: Mouse, Human, Recombinant fragment -
免疫原
Recombinant fragment corresponding to Human DLK-1 aa 174-349.
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阳性对照
- Recombinant DLK-1 protein; U251 cells; NIH3T3 cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
Purified from tissue culture supernatant. -
克隆
单克隆 -
克隆编号
3A10 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab119930于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/2000. Predicted molecular weight: 41 kDa.
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ELISA |
1/10000.
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Flow Cyt |
1/200 - 1/400.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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ICC/IF | (2) |
1/200 - 1/1000.
|
说明 |
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WB
1/500 - 1/2000. Predicted molecular weight: 41 kDa. |
ELISA
1/10000. |
Flow Cyt
1/200 - 1/400. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ICC/IF
1/200 - 1/1000. |
靶标
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功能
May have a role in neuroendocrine differentiation. -
组织特异性
Found within the stromal cells in close contact to the vascular structure of placental villi, yolk sac, fetal liver, adrenal cortex and pancreas and in the beta cells of the islets of Langerhans in the adult pancreas. Found also in some forms of neuroendocrine lung tumor tissue. -
序列相似性
Contains 6 EGF-like domains. -
翻译后修饰
N- and O-glycosylated. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 8788 Human
- Entrez Gene: 13386 Mouse
- Omim: 176290 Human
- SwissProt: P80370 Human
- SwissProt: Q09163 Mouse
- Unigene: 533717 Human
- Unigene: 157069 Mouse
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别名
- Adipocyte differentiation inhibitor protein antibody
- Brevideltin antibody
- Delta like 1 homolog antibody
see all
图片
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Immunofluorescence analysis of U251 cells using ab119930 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Anti-DLK-1 antibody [3A10] (ab119930) at 1/500 dilution + Recombinant DLK-1 protein
Predicted band size: 41 kDa -
Flow cytometric analysis of NIH3T3 cells using ab119930 at 1/200 dilution (green) and negative control (red).
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ELISA using ab119930.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (12)
ab119930 被引用在 12 文献中.
- Bondareva O et al. Single-cell profiling of vascular endothelial cells reveals progressive organ-specific vulnerabilities during obesity. Nat Metab 4:1591-1610 (2022). PubMed: 36400935
- Li Y et al. LncRNA LINC01315 silencing modulates cancer stem cell properties and epithelial-to-mesenchymal transition in colorectal cancer via miR-484/DLK1 axis. Cell Cycle 21:851-873 (2022). PubMed: 35156543
- Eze UC et al. Single-cell atlas of early human brain development highlights heterogeneity of human neuroepithelial cells and early radial glia. Nat Neurosci 24:584-594 (2021). PubMed: 33723434
- Shen AH et al. Prophylactic treatment with transdermal deferoxamine mitigates radiation-induced skin fibrosis. Sci Rep 10:12346 (2020). PubMed: 32704071
- Wise LM et al. Anti-fibrotic Actions of Equine Interleukin-10 on Transforming Growth Factor-Beta1-Stimulated Dermal Fibroblasts Isolated From Limbs of Horses. Front Vet Sci 7:577835 (2020). PubMed: 33195583