AB109416

Anti-DKK1 抗体 [EPR4759]

Name: DKK1抗体[EPR4759] (ab109416)| Abcam中文官网
Brand: Abcam Limited
SKU: AB109416
Price: 2644 CNY
Availability: InStock
Rating: 2 (1 reviews)

Anti-DKK1 antibody [EPR4759]

RabMAb
Recombinant
了解详情

(1 Review)

(57 Publications)

Rabbit Recombinant Monoclonal DKK1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 57 publications.

查看别名

UNQ492/PRO1008, DKK1, Dickkopf-related protein 1, Dickkopf-1, Dkk-1, hDkk-1, SK

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DKK1 antibody [EPR4759] (AB109416)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DKK1 antibody [EPR4759] (AB109416)

Immunohistochemical analysis of paraffin-embedded Human placenta tissue using 1/100 unpurified ab109416.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DKK1 antibody [EPR4759] (AB109416)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling DKK1 with Purified ab109416 at 1 : 800 dilution (1.04 µg/ml). Heat mediated antigen retrieval was performed using using ab93684 (Tris/EDTA buffer, pH 9.0)ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control : PBS instead of the primary antibody.Hematoxylinwas used as a counterstain

Lab

Western blot - Anti-DKK1 antibody [EPR4759] (AB109416)

False colour image of Western blot : Anti-DKK1 antibody [EPR4759] staining at 1/2000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-DKK1 antibody [EPR4759] (ab109416) at 1/2000 dilution

Lane 1:

Caco-2 cell lysate at 20 µg

Lane 2:

PC-3 cell lysate at 20 µg

Lane 3:

U-2 OS cell lysate at 20 µg

Lane 4:

MOLT4 cell lysate at 20 µg

Lane 5:

HEK-293 cell lysate at 20 µg

Predicted band size: 29 kDa

Observed band size: 30-45 kDa

false

Lab

Western blot - Anti-DKK1 antibody [EPR4759] (AB109416)

Blocking/Diluting buffer and concentration 5% NFDM/TBST.

HEK-293 and 293T cell are weak expressed cell lines for DKK1 protein (PMID : 18502762, PMID : 15378020).

All lanes:

Western blot - Anti-DKK1 antibody [EPR4759] (ab109416) at 1/10000 dilution

Lane 1:

Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 29 kDa

Observed band size: 29-40 kDa

false

Exposure time: 180s

Unknown

Western blot - Anti-DKK1 antibody [EPR4759] (AB109416)

Blocking and diluting buffer : 5% NFDM/TBST.

The smear bands detected by ab109416 are similar to those reported by the papers (PMID : 18546262, 28090290, 24008318), which should be due to the glycosylation of DKK1.

All lanes:

Western blot - Anti-DKK1 antibody [EPR4759] (ab109416) at 1/2000 dilution

Lane 1:

A549 (Human lung carcinoma epithelial cell) whole cell lysates at 20 µg

Lane 2:

PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysates at 20 µg

Lane 3:

NCI-H460 (Human Large Cell Lung Cancer epithelial cell) whole cell lysates at 20 µg

Lane 4:

DU145 (Human prostate carcinoma epithelial cell) whole cell lysates at 20 µg

Lane 5:

SK-OV-3 (Human ovarian cancer epithelial cell) whole cell lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 29 kDa

false

Unknown

Western blot - Anti-DKK1 antibody [EPR4759] (AB109416)

Blocking/Diluting buffer and concentration 5% NFDM /TBST

Exposure time : 5.5 seconds

All lanes:

Western blot - Anti-DKK1 antibody [EPR4759] (ab109416) at 1/1000 dilution

All lanes:

His-Tagged human DKK1 (aa 32 to 266) recombinant protein, 100ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 29 kDa

false

不同偶联物与剂型 (2)

Carrier free

Anti-DKK1 antibody [EPR4759] - BSA and Azide free
Carrier free

Anti-DKK1 antibody [EPR4759] - Low endotoxin, Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR4759

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

Although our internal data shows that this antibody is not suitable to detect DKK1 in rats, this antibody has been referenced in PMID 29299122 and 25469240 to work with rat derived cells. Please contact technical@abcam.com for further information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/800", "IHCP-species-notes": "1/250 - 1/500 Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The DKK1 protein also known as Dickkopf-1 is a secreted protein with a mass of approximately 28-30 kDa. It plays a mechanical role in inhibiting the Wnt signaling pathway by binding to LRP5/6 co-receptors preventing Wnt ligands from activating the pathway. DKK1 is expressed in various tissues including the bone brain and placenta reflecting its involvement in several biological processes. Its capacity to bind and neutralize the Wnt pathway components cements DKK1's role as a significant modulator of cellular functions.

Biological function summary

The inhibition of the Wnt signaling by DKK1 alters cell proliferation differentiation and migration. DKK1 does not function as part of a large multi-protein complex but interacts directly with specific co-receptors and modulates the activity of key signaling molecules. This regulatory mechanism controls processes like bone formation and neurogenesis indicating DKK1's broad impact in development and tissue homeostasis. Its expression pattern and biological role position it as an important player in maintaining proper cellular signaling environments.

Pathways

DKK1 is a critical player in the Wnt signaling and bone remodeling pathways. Through its interaction with Wnt pathway components like LRP5/6 DKK1 modulates these pathways affecting downstream proteins such as β-catenin and influencing gene transcription. In the bone remodeling context DKK1's ability to regulate osteoblast activity connects it to other related proteins like sclerostin contributing to bone density and health.

Elevated levels of DKK1 are observed in conditions like osteoporosis and cancer. In osteoporosis DKK1's inhibition of Wnt signaling leads to reduced bone formation implicating it alongside proteins like sclerostin in deteriorating bone health. In cancer DKK1's dysregulation can promote tumorigenesis by altering cell signaling and microenvironment interactions. These roles underline the potential of targeting DKK1 for therapeutic purposes in treating these diseases.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6 (PubMed : 22000856). DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (PubMed : 17143291). Inhibits the pro-apoptotic function of KREMEN1 in a Wnt-independent manner, and has anti-apoptotic activity (By similarity).

See full target information DKK1

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文献 (57)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 30:208 PubMed40927640

2025

Tetramethylpyrazine alleviates acute kidney injury by activating the Wnt/β-catenin pathway independent of DKK1.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohui Wang,Xiaoxia Chang,Donglin Yang,Lixia Zhang,Zijie Guo,Xuhong Sun,Aiqun Li,Yanbo Ni,Pengchao Du

Frontiers in bioengineering and biotechnology 13:1521134 PubMed39995594

2025

Gingival fibroblast suppress the osteogenesis process mediated by bone substitute materials via WNT/β-catenin signaling pathway and .

Applications

Unspecified application

Species

Unspecified reactive species

Guanqi Liu,Jiahui Lin,Xiaoyan Chen,Runheng Liu

Cell death & disease 15:797 PubMed39505867

2024

DKK1+ tumor cells inhibited the infiltration of CCL19+ fibroblasts and plasma cells contributing to worse immunotherapy response in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Guangyu Fan,Ruyun Gao,Tongji Xie,Lin Li,Le Tang,Xiaohong Han,Yuankai Shi

MedComm 5:e518 PubMed38525111

2024

Dickkopf-1 drives perineural invasion via PI3K-AKT signaling pathway in head and neck squamous cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jingyi Wang,Qianying Li,Faya Liang,Xin Du,Pan Song,Taowei Wu,Renhui Chen,Xiaorong Lin,Qinglian Liu,Hai Hu,Ping Han,Xiaoming Huang

Cancer medicine 13:e6992 PubMed38334454

2024

Dickkopf 1 is expressed in normal fibroblasts during early stages of colorectal tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yushi Inomata,Masatake Kuroha,Yusuke Shimoyama,Takeo Naito,Rintaro Moroi,Hisashi Shiga,Yoichi Kakuta,Hideaki Karasawa,Shinobu Onuma,Yoshitaka Kinouchi,Atsushi Masamune

Biology direct 18:34 PubMed37365579

2023

Dynamic expression of IGFBP3 modulate dual actions of mineralization micro-environment during tooth development via Wnt/beta-catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

MengDan Zhang,Junming Zheng,Siyuan Wu,Hailing Chen,Lusai Xiang

Journal of bone and mineral metabolism 41:481-491 PubMed37247112

2023

Mir-4699 promotes the osteogenic differentiation of mesenchymal stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Vahedeh Hosseini,Mahdi Paryan,Ameneh Koochaki,Henry Manuel Cesaire,Samira Mohammadi-Yeganeh

Clinical Medicine Insights. Oncology 17:11795549231168073 PubMed37114075

2023

Correlation of NNMT and DKK1 Protein Expression With Clinicopathological Characteristics and Prognosis of Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Tairong Liang,Xiuqian Wu,Lan Wang,Tiantian Song,Peishan Wu,Yongdong Niu,Haihua Huang

International journal of molecular sciences 24: PubMed37108433

2023

Mild Chronic Kidney Disease Associated with Low Bone Formation and Decrease in Phosphate Transporters and Signaling Pathways Gene Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Evdokia Bogdanova,Airat Sadykov,Galina Ivanova,Irina Zubina,Olga Beresneva,Natalia Semenova,Olga Galkina,Marina Parastaeva,Vladimir Sharoyko,Vladimir Dobronravov

BMC musculoskeletal disorders 24:246 PubMed36997896

2023

Increased synovial immunohistochemistry reactivity of TGF-β1 in erosive peripheral psoriatic arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Jose A Pinto Tasende,M Fernandez-Moreno,M E Vazquez-Mosquera,J C Fernandez-Lopez,N Oreiro-Villar,F J De Toro Santos,F J Blanco-García

View all publications

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