Anti-DKK1抗体(ab61034)
Key features and details
- Rabbit polyclonal to DKK1
- Suitable for: IHC-Fr, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-DKK1抗体
参阅全部 DKK1 一抗 -
描述
兔多克隆抗体to DKK1 -
宿主
Rabbit -
经测试应用
适用于: IHC-Fr, IHC-Pmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide (Human) from the internal domain of DKK1 conjugated to KLH
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.70
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab61034于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-Fr | (2) |
Use at an assay dependent concentration.
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| IHC-P | (3) |
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C for 20 minutes. Remove from heat and let stand at room temperature in buffer for 20 minutes. Rinse in 1X TBS with Tween (TBST) for 1 minute at RT. |
| 说明 |
|---|
|
IHC-Fr
Use at an assay dependent concentration. |
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IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C for 20 minutes. Remove from heat and let stand at room temperature in buffer for 20 minutes. Rinse in 1X TBS with Tween (TBST) for 1 minute at RT. |
靶标
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功能
Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. -
组织特异性
Placenta. -
序列相似性
Belongs to the dickkopf family. -
结构域
The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 22943 Human
- Entrez Gene: 13380 Mouse
- Omim: 605189 Human
- SwissProt: O94907 Human
- SwissProt: O54908 Mouse
- Unigene: 40499 Human
- Unigene: 214717 Mouse
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别名
- Dickkopf 1 antibody
- Dickkopf 1 homolog antibody
- Dickkopf 1 like antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DKK1 antibody (ab61034)
ab61034 at 10µg/ml staining formalin fixed paraffin embedded Human Uterus (endometrium).
数据表及文件
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SDS download
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Datasheet download
文献 (22)
ab61034 被引用在 22 文献中.
- Suda T et al. Dickkopf-1 Promotes Angiogenesis and is a Biomarker for Hepatic Stem Cell-like Hepatocellular Carcinoma. Int J Mol Sci 23:N/A (2022). PubMed: 35269944
- Kerschan-Schindl K et al. Differential Expression of Dickkopf 1 and Periostin in Mouse Strains with High and Low Bone Mass. Biology (Basel) 11:N/A (2022). PubMed: 36552348
- Bargagna-Mohan P et al. Corneal nonmyelinating Schwann cells illuminated by single-cell transcriptomics and visualized by protein biomarkers. J Neurosci Res 99:731-749 (2021). PubMed: 33197966
- Sugali CK et al. The Canonical Wnt Signaling Pathway Inhibits the Glucocorticoid Receptor Signaling Pathway in the Trabecular Meshwork. Am J Pathol 191:1020-1035 (2021). PubMed: 33705750
- Pal A et al. EHMT2 epigenetically suppresses Wnt signaling and is a potential target in embryonal rhabdomyosarcoma. Elife 9:N/A (2020). PubMed: 33252038