Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
重组RabMAb

重组Anti-Dishevelled 3抗体[EP1991Y] (ab76081)

Reviews (2)Q&A (2)References (8)
Western blot - Anti-Dishevelled 3 antibody [EP1991Y] (ab76081)
  • Immunocytochemistry/ Immunofluorescence - Anti-Dishevelled 3 antibody [EP1991Y] (ab76081)
  • Flow Cytometry (Intracellular) - Anti-Dishevelled 3 antibody [EP1991Y] (ab76081)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1991Y] to Dishevelled 3
  • Suitable for: WB, ICC/IF, Flow Cyt (Intra)
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-Dishevelled 3抗体[EP1991Y]
    参阅全部 Dishevelled 3 一抗

  • 描述

    兔单克隆抗体[EP1991Y] to Dishevelled 3

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, ICC/IF, Flow Cyt (Intra)more details
    不适用于: IP

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: K-562, RAW 264.7, and C6 whole cell lysates. ICC/IF: PC-12 cells. Flow Cyt (Intra): HeLa cells.

  • 常规说明

    This product has switched from a hybridoma to recombinant production method on 13th September 2024.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab76081于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (1)
1/1000. Predicted molecular weight: 78 kDa.
ICC/IF
1/50.
Flow Cyt (Intra)
1/200.
说明
WB
1/1000. Predicted molecular weight: 78 kDa.
ICC/IF
1/50.
Flow Cyt (Intra)
1/200.
应用说明
Is unsuitable for IP.

靶标

  • 功能

    May play a role in the signal transduction pathway mediated by multiple Wnt genes.

  • 序列相似性

    Belongs to the DSH family.
    Contains 1 DEP domain.
    Contains 1 DIX domain.
    Contains 1 PDZ (DHR) domain.

  • 翻译后修饰

    Ubiquitinated. Deubiquitinated by CYLD, which acts on 'Lys-63'-linked ubiquitin chains.

  • 细胞定位

    Cytoplasm.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • Dishevelled 3 (homologous to Drosophila dsh) antibody
    • Dishevelled 3 antibody
    • Dishevelled 3, dsh homolog (Drosophila) antibody
    • Dishevelled, dsh homolog 3 (Drosophila) antibody
    • Dishevelled-3 antibody
    • DSH homolog 3 antibody
    • dvl3 antibody
    • DVL3_HUMAN antibody
    • KIAA0208 antibody
    • OTTHUMP00000210777 antibody
    • OTTHUMP00000210778 antibody
    • Segment polarity protein dishevelled homolog DVL 3 antibody
    • Segment polarity protein dishevelled homolog DVL-3 antibody
    see all

图片

  • Western blot - Anti-Dishevelled 3 antibody [EP1991Y] (ab76081)
    Western blot - Anti-Dishevelled 3 antibody [EP1991Y] (ab76081)
    All lanes : Anti-Dishevelled 3 antibody [EP1991Y] (ab76081) at 1/1000 dilution

    Lane 1 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate
    Lane 2 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
    Lane 3 : C6 (Rat glial tumor glial cell) whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 78 kDa
    Observed band size: 88-93 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: Lane 1, 20 seconds; Lane 2 and Lane 3, 40 seconds.

    Double bands of DVL3 could be observed which was supposed to be caused by phosphorylation supported by literatures (PMID: 31870452, 22647544).

  • Immunocytochemistry/ Immunofluorescence - Anti-Dishevelled 3 antibody [EP1991Y] (ab76081)
    Immunocytochemistry/ Immunofluorescence - Anti-Dishevelled 3 antibody [EP1991Y] (ab76081)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized PC-12 cells labelling Dishevelled 3 with ab76081 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) at 1/1000 dilution at RT for 45 min. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889) was used to counterstain tubulin at 1/200 dilution. Nucleus were visualized using DAPI.

  • Flow Cytometry (Intracellular) - Anti-Dishevelled 3 antibody [EP1991Y] (ab76081)
    Flow Cytometry (Intracellular) - Anti-Dishevelled 3 antibody [EP1991Y] (ab76081)

    Flow cytometry analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) labelling Dishevelled 3 with ab76081 at 1/200 dilution, followed by blocking with 10% goat serum for 1h at room temperature. ab76081 was incubated for 30 min at room temperature (RT) and then Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150081) was incubated for 30 min at room temperature.

文献 (8)

发表研究结果有使用 ab76081？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab76081 被引用在 8 文献中.

  • Asif M  et al. De novo variants of CSNK2B cause a new intellectual disability-craniodigital syndrome by disrupting the canonical Wnt signaling pathway. HGG Adv 3:100111 (2022). PubMed: 35571680
  • Pruller J  et al. DVL1 and DVL3 require nuclear localisation to regulate proliferation in human myoblasts. Sci Rep 12:8388 (2022). PubMed: 35589804
  • Zeng X  et al. Secreted Frizzled Related Protein 2 Modulates Epithelial-Mesenchymal Transition and Stemness via Wnt/ß-Catenin Signaling in Choriocarcinoma. Cell Physiol Biochem 50:1815-1831 (2018). PubMed: 30396168
  • Hua Y  et al. Oligomerization of Frizzled and LRP5/6 protein initiates intracellular signaling for the canonical WNT/ß-catenin pathway. J Biol Chem 293:19710-19724 (2018). PubMed: 30361437
  • Wu Y  et al. Low expression of secreted frizzled-related protein 2 and nuclear accumulation of ß-catenin in aggressive nonfunctioning pituitary adenoma. Oncol Lett 12:199-206 (2016). WB ; Human . PubMed: 27347125
  • He H  et al. miR-204-5p promotes the adipogenic differentiation of human adipose-derived mesenchymal stem cells by modulating DVL3 expression and suppressing Wnt/ß-catenin signaling. Int J Mol Med 35:1587-95 (2015). WB . PubMed: 25847080
  • Lin X  et al. Differential Expression of Wnt Pathway Genes in Sporadic Hepatocellular Carcinomas Infected With Hepatitis B Virus Identified With OligoGE Arrays. Hepat Mon 13:e6192 (2013). PubMed: 23483081
  • Burcklé C  et al. Control of the Wnt pathways by nephrocystin-4 is required for morphogenesis of the zebrafish pronephros. Hum Mol Genet 20:2611-27 (2011). IHC (PFA fixed) . PubMed: 21498478

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

Western blot

 Good
Abreviews
abreview image
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (12 % SDS PAGE gel)
Sample
Human Cell lysate - whole cell (HEK 293T cells)
Specification
HEK 293T cells
Treatment
transfected with Dvl-3 plasmid
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 May 23 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com