AB76081

Anti-Dishevelled 3抗体[EP1991Y]

Name: Dishevelled 3抗体[EP1991Y] (ab76081)| Abcam中文官网
Brand: Abcam Limited
SKU: AB76081
Price: 3097 CNY
Availability: InStock
Rating: 4 (2 reviews)

Anti-Dishevelled 3 antibody [EP1991Y]

RabMAb
了解详情

(2 Reviews)

(8 Publications)

Rabbit Monoclonal Dishevelled 3 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 8 publications.

查看别名

KIAA0208, DVL3, Segment polarity protein dishevelled homolog DVL-3, Dishevelled-3, DSH homolog 3

3 Images

Flow Cytometry (Intracellular) - Anti-Dishevelled 3 antibody [EP1991Y] (AB76081)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Dishevelled 3 antibody [EP1991Y] (AB76081)

Flow cytometry analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) labelling Dishevelled 3 with ab76081 at 1/200 dilution, followed by blocking with 10% goat serum for 1h at room temperature. ab76081 was incubated for 30 min at room temperature (RT) and then Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150081) was incubated for 30 min at room temperature.

Immunocytochemistry/ Immunofluorescence - Anti-Dishevelled 3 antibody [EP1991Y] (AB76081)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Dishevelled 3 antibody [EP1991Y] (AB76081)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized PC-12 cells labelling Dishevelled 3 with ab76081 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) at 1/1000 dilution at RT for 45 min. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889) was used to counterstain tubulin at 1/200 dilution. Nucleus were visualized using DAPI.

Lab

Western blot - Anti-Dishevelled 3 antibody [EP1991Y] (AB76081)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : Lane 1, 20 seconds; Lane 2 and Lane 3, 40 seconds.

Double bands of DVL3 could be observed which was supposed to be caused by phosphorylation supported by literatures (PMID : 31870452, 22647544).

All lanes:

Western blot - Anti-Dishevelled 3 antibody [EP1991Y] (ab76081) at 1/1000 dilution

Lane 1:

K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate at 15 µg

Lane 2:

RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 15 µg

Lane 3:

C6 (Rat glial tumor glial cell) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

false

不同偶联物与剂型 (1)

Carrier free

Anti-Dishevelled 3 antibody [EP1991Y] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EP1991Y

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

ICC/IF, Flow Cyt (Intra), WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/30", "FlowCytIntra-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Involved in the signal transduction pathway mediated by multiple Wnt genes.

See full target information DVL3

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文献 (8)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 12:8388 PubMed35589804

2022

DVL1 and DVL3 require nuclear localisation to regulate proliferation in human myoblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Johanna Pruller,Nicolas Figeac,Peter S Zammit

HGG advances 3:100111 PubMed35571680

2022

variants of cause a new intellectual disability-craniodigital syndrome by disrupting the canonical Wnt signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Asif,Emrah Kaygusuz,Marwan Shinawi,Anna Nickelsen,Tzung-Chien Hsieh,Prerana Wagle,Birgit S Budde,Jennifer Hochscherf,Uzma Abdullah,Stefan Höning,Christian Nienberg,Dirk Lindenblatt,Angelika A Noegel,Janine Altmüller,Holger Thiele,Susanne Motameny,Nicole Fleischer,Idan Segal,Lynn Pais,Sigrid Tinschert,Nadra Nasser Samra,Juliann M Savatt,Natasha L Rudy,Chiara De Luca, Paola Fortugno,Susan M White,Peter Krawitz,Anna C E Hurst,Karsten Niefind,Joachim Jose,Francesco Brancati,Peter Nürnberg,Muhammad Sajid Hussain

Cellular physiology and biochemistry : internation 50:1815-1831 PubMed30396168

2018

Secreted Frizzled Related Protein 2 Modulates Epithelial-Mesenchymal Transition and Stemness via Wnt/β-Catenin Signaling in Choriocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xianling Zeng,Yafei Zhang,Huiqiu Xu,Taohong Zhang,Yan Xue,Ruifang An

The Journal of biological chemistry 293:19710-19724 PubMed30361437

2018

Oligomerization of Frizzled and LRP5/6 protein initiates intracellular signaling for the canonical WNT/β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Hua,Yilin Yang,Qian Li,Xinyu He,Wei Zhu,Jiyong Wang,Xiaoqing Gan

Oncology letters 12:199-206 PubMed27347125

2016

Low expression of secreted frizzled-related protein 2 and nuclear accumulation of β-catenin in aggressive nonfunctioning pituitary adenoma.

Applications

Species

Human

Youtu Wu,Jiwei Bai,Linchuan Hong,Chunhui Liu,Shengyuan Yu,Guoqiang Yu,Yazhuo Zhang

International journal of molecular medicine 35:1587-95 PubMed25847080

2015

miR-204-5p promotes the adipogenic differentiation of human adipose-derived mesenchymal stem cells by modulating DVL3 expression and suppressing Wnt/β-catenin signaling.

Applications

Species

Unspecified reactive species

Honghui He,Ke Chen,Fang Wang,Liling Zhao,Xinxing Wan,Linghao Wang,Zhaohui Mo

Hepatitis monthly 13:e6192 PubMed23483081

2013

Differential Expression of Wnt Pathway Genes in Sporadic Hepatocellular Carcinomas Infected With Hepatitis B Virus Identified With OligoGE Arrays.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyan Lin,Qiangxiu Wang,Zhixin Cao,Ming Geng,Yongcheng Cao,Xiaohong Liu

Human molecular genetics 20:2611-27 PubMed21498478

2011

Control of the Wnt pathways by nephrocystin-4 is required for morphogenesis of the zebrafish pronephros.

Applications

IHC (PFA fixed)

Species

Unspecified reactive species

Céline Burcklé,Helori-Mael Gaudé,Christine Vesque,Flora Silbermann,Rémi Salomon,Cécile Jeanpierre,Corinne Antignac,Sophie Saunier,Sylvie Schneider-Maunoury

View all publications

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Anti-Dishevelled 3抗体[EP1991Y]